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- W2997157466 abstract "Abstract Light scattering detection in microfluidic chips provides an important tool to identify cancer cells without any label processes. However, forward small‐angle scattering signals of cells, which are related to their sizes and morphologies, are hard to be detected accurately when scattering angle is less than 11° in microfluidic chips by traditional lighting design due to the influence of incident beam. Therefore, cell's size and morphology being the golden standard for clinical detection may lose their efficacy in recognizing cancer cells from healthy ones. In this article, a novel lighting design in microfluidic chips is put forward in which traditional incident Gaussian beam can be modulated into quasi‐Bessel beam by a microprism and waveguide. The quasi‐Bessel beam's advantages of nondiffraction theoretically make forward scattering (FS) detection less than 11° possibly. Our experimental results for peripheral blood lymphocytes of human beings and cultured HeLa cells show that the detection rates increase by 47.87% and 46.79%, respectively, by the novel designed microfluidic chip compared to traditional Gaussian lighting method in microfluidic chips. © 2019 International Society for Advancement of Cytometry" @default.
- W2997157466 created "2020-01-10" @default.
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- W2997157466 date "2019-12-25" @default.
- W2997157466 modified "2023-09-26" @default.
- W2997157466 title "A Design of Microfluidic Chip with Quasi‐Bessel Beam Waveguide for Scattering Detection of Label‐Free Cancer Cells" @default.
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- W2997157466 doi "https://doi.org/10.1002/cyto.a.23954" @default.
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