Matches in SemOpenAlex for { <https://semopenalex.org/work/W2997162332> ?p ?o ?g. }
- W2997162332 endingPage "1999" @default.
- W2997162332 startingPage "1985" @default.
- W2997162332 abstract "Abstract A number of regulatory nascent peptides have been shown to regulate gene expression by causing programmed ribosome stalling during translation. Nascent peptide emerges from the ribosome through the exit tunnel, and one-third of the way along which β-loop structures of ribosomal proteins uL4 and uL22 protrude into the tunnel to form the constriction region. Structural studies have shown interactions between nascent peptides and the exit tunnel components including the constriction region. In eukaryotes, however, there is a lack of genetic studies for the involvement of the constriction region in ribosome stalling. Here, we established transgenic Arabidopsis lines that carry mutations in the β-loop structure of uL4. Translation analyses using a cell-free translation system derived from the transgenic Arabidopsis carrying the mutant ribosome showed that the uL4 mutations reduced the ribosome stalling of four eukaryotic stalling systems, including those for which stalled structures have been solved. Our data, which showed differential effects of the uL4 mutations depending on the stalling systems, explained the spatial allocations of the nascent peptides at the constriction that were deduced by structural studies. Conversely, our data may predict allocation of the nascent peptide at the constriction of stalling systems for which structural studies are not done." @default.
- W2997162332 created "2020-01-10" @default.
- W2997162332 creator A5011430788 @default.
- W2997162332 creator A5018364617 @default.
- W2997162332 creator A5030876154 @default.
- W2997162332 creator A5037748179 @default.
- W2997162332 creator A5039938027 @default.
- W2997162332 creator A5047611446 @default.
- W2997162332 creator A5052311523 @default.
- W2997162332 creator A5058609018 @default.
- W2997162332 creator A5063207088 @default.
- W2997162332 creator A5088083152 @default.
- W2997162332 date "2019-12-25" @default.
- W2997162332 modified "2023-10-17" @default.
- W2997162332 title "Reverse genetics-based biochemical studies of the ribosomal exit tunnel constriction region in eukaryotic ribosome stalling: spatial allocation of the regulatory nascent peptide at the constriction" @default.
- W2997162332 cites W1489656307 @default.
- W2997162332 cites W1491077584 @default.
- W2997162332 cites W1528915146 @default.
- W2997162332 cites W1849730822 @default.
- W2997162332 cites W1899841999 @default.
- W2997162332 cites W1975616739 @default.
- W2997162332 cites W1978621360 @default.
- W2997162332 cites W1983332099 @default.
- W2997162332 cites W1992282744 @default.
- W2997162332 cites W1996868085 @default.
- W2997162332 cites W1998053175 @default.
- W2997162332 cites W2001118852 @default.
- W2997162332 cites W2004901127 @default.
- W2997162332 cites W2016978669 @default.
- W2997162332 cites W2026033633 @default.
- W2997162332 cites W2038027259 @default.
- W2997162332 cites W2050576371 @default.
- W2997162332 cites W2060913542 @default.
- W2997162332 cites W2063381787 @default.
- W2997162332 cites W2063742123 @default.
- W2997162332 cites W2078290510 @default.
- W2997162332 cites W2085803345 @default.
- W2997162332 cites W2086084094 @default.
- W2997162332 cites W2086225599 @default.
- W2997162332 cites W2092985726 @default.
- W2997162332 cites W2095848833 @default.
- W2997162332 cites W2097654784 @default.
- W2997162332 cites W2098115340 @default.
- W2997162332 cites W2100654918 @default.
- W2997162332 cites W2103666721 @default.
- W2997162332 cites W2116122104 @default.
- W2997162332 cites W2122964499 @default.
- W2997162332 cites W2126437265 @default.
- W2997162332 cites W2127841826 @default.
- W2997162332 cites W2127994165 @default.
- W2997162332 cites W2135360731 @default.
- W2997162332 cites W2136484632 @default.
- W2997162332 cites W2137622016 @default.
- W2997162332 cites W2142111672 @default.
- W2997162332 cites W2142482793 @default.
- W2997162332 cites W2149918090 @default.
- W2997162332 cites W2150598386 @default.
- W2997162332 cites W2155167836 @default.
- W2997162332 cites W2157406319 @default.
- W2997162332 cites W2163046455 @default.
- W2997162332 cites W2163448850 @default.
- W2997162332 cites W2163834574 @default.
- W2997162332 cites W2164775480 @default.
- W2997162332 cites W2168458051 @default.
- W2997162332 cites W2220104844 @default.
- W2997162332 cites W2254067232 @default.
- W2997162332 cites W2269683575 @default.
- W2997162332 cites W2271498058 @default.
- W2997162332 cites W2333995371 @default.
- W2997162332 cites W2414624004 @default.
- W2997162332 cites W2427010553 @default.
- W2997162332 cites W2511774689 @default.
- W2997162332 cites W2534128196 @default.
- W2997162332 cites W2534583417 @default.
- W2997162332 cites W2603871277 @default.
- W2997162332 cites W2626494417 @default.
- W2997162332 cites W370978 @default.
- W2997162332 cites W998025526 @default.
- W2997162332 doi "https://doi.org/10.1093/nar/gkz1190" @default.
- W2997162332 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/7038982" @default.
- W2997162332 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/31875230" @default.
- W2997162332 hasPublicationYear "2019" @default.
- W2997162332 type Work @default.
- W2997162332 sameAs 2997162332 @default.
- W2997162332 citedByCount "3" @default.
- W2997162332 countsByYear W29971623322020 @default.
- W2997162332 countsByYear W29971623322021 @default.
- W2997162332 crossrefType "journal-article" @default.
- W2997162332 hasAuthorship W2997162332A5011430788 @default.
- W2997162332 hasAuthorship W2997162332A5018364617 @default.
- W2997162332 hasAuthorship W2997162332A5030876154 @default.
- W2997162332 hasAuthorship W2997162332A5037748179 @default.
- W2997162332 hasAuthorship W2997162332A5039938027 @default.
- W2997162332 hasAuthorship W2997162332A5047611446 @default.
- W2997162332 hasAuthorship W2997162332A5052311523 @default.
- W2997162332 hasAuthorship W2997162332A5058609018 @default.
- W2997162332 hasAuthorship W2997162332A5063207088 @default.
- W2997162332 hasAuthorship W2997162332A5088083152 @default.