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• W2997378426 abstract "The xenograft of human cancer cells in model animals is a powerful tool for understanding tumor progression and metastatic potential. Mice represent a validated host, but their use is limited by the elevated experimental costs and low throughput. To overcome these restrictions, zebrafish larvae might represent a valuable alternative. Their small size and transparency allow the tracking of transplanted cells. Therefore, tumor growth and early steps of metastasis, which are difficult to evaluate in mice, can be addressed. In spite of its advantages, the use of this model has been hindered by lack of experimental homogeneity and validation. Considering these facts, the aim of our work was to standardize, automate, and validate a zebrafish larvae xenograft assay with increased translatability and higher drug screening throughput. The ZeOncoTest reliability is based on the optimization of different experimental parameters, such as cell labeling, injection site, automated individual sample image acquisition, and analysis. This workflow implementation finally allows a higher precision and experimental throughput increase, when compared to previous reports. The approach was validated with the breast cancer cell line MDA-MB-231, the colorectal cancer cells HCT116, and the prostate cancer cells PC3; and known drugs, respectively RKI-1447, Docetaxel, and Mitoxantrone. The results recapitulate growth and invasion for all tested tumor cells, along with expected efficacy of the compounds. Finally, the methodology has proven useful for understanding specific drugs mode of action. The insights gained bring a step further for zebrafish larvae xenografts to enter the regulated preclinical drug discovery path." @default.
• W2997378426 created "2020-01-10" @default.
• W2997378426 creator A5024730948 @default.
• W2997378426 creator A5063044833 @default.
• W2997378426 creator A5065020120 @default.
• W2997378426 creator A5073036317 @default.
• W2997378426 date "2019-12-24" @default.
• W2997378426 modified "2023-10-06" @default.
• W2997378426 title "ZeOncoTest: Refining and Automating the Zebrafish Xenograft Model for Drug Discovery in Cancer" @default.
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• W2997378426 doi "https://doi.org/10.3390/ph13010001" @default.
• W2997378426 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/7169390" @default.
• W2997378426 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/31878274" @default.
• W2997378426 hasPublicationYear "2019" @default.
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