FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2997392911> ?p ?o ?g. }

• W2997392911 endingPage "3644" @default.
• W2997392911 startingPage "3638" @default.
• W2997392911 abstract "Objective: To investigate the role of transcription factor peroxisome proliferator-activated receptor-gamma coactivator-1 beta (PGC-1β) on osteoclastogenesis and related regulatory mechanism in the mouse monocyte-macrophage cell line (RAW264.7). Methods: PGC-1β expression and location in RAW264.7 cells was detected by immunofluorescence, flow cytometry and western blot analysis with nuclear protein extraction. RAW264.7 cells were transfected with lentivirus for gene silencing or over-expression of PGC-1β and cultured with macrophage colony-stimulating factor and receptor activator for nuclear factor-κB ligand. Cell viability was detected by cell counting kit-8. Cell apoptosis and cell cycle were detected by flow cytometry. Mature osteoclasts and their bone resorption activity were determined by tartrate-resistant acid phosphatase (TRAP) expression and toluidine blue staining. Western blot analysis was performed for detecting dendritic cell-specific transmembrane protein (DC-STAMP), cathepsin K, TRAP and matrix metalloproteinase (MMP)-9 expression, as well as cytoplasmic NF-κB-inducing kinase (NIK) and nuclear RelB. Results: PGC-1β expression was observed in the nuclei of RAW264.7 cells. Down-regulation or overexpression of PGC-1β in RAW264.7 cells did not affect cell viability, apoptosis or cell cycle. Down-regulation of PGC-1β decreased the count of mature osteoclasts (49±21 cells vs. 147±42 cells, P=0.004) and the pit area of bone resorption lacunae (42.11μm(2)±11.30 μm(2) vs. 204.80μm(2)±31.09 μm(2), P<0.001), as well as the expression of cathepsin K, TRAP and MMP-9, but not DC-STAMP. Overexpression of PGC-1β promoted osteoclast differentiation and bone resorption activity, as well as the expression of cathepsin K, TRAP and MMP-9. Down-regulation of PGC-1β suppressed the protein expression of cytoplasmic NIK and nuclear RelB in RAW264.7 cells. Conclusion: PGC-1β can promote the differentiation of RAW264.7 into osteoclasts and improve the bone resorption ability of the cells via activation of NIK/RelB pathway, which might be a promising therapeutic target for osteoporosis.目的： 探讨过氧化物酶体增殖物激活受体γ辅激活因子（PGC）-1β在诱导小鼠单核巨噬细胞（RAW264.7）向破骨细胞分化及活化的调控作用及机制。 方法： 体外培养RAW264.7细胞，慢病毒转染下调或过表达PGC-1β后采用巨噬细胞集落刺激因子和重组细胞核因子κB受体活化因子配体体外诱导RAW264.7细胞向破骨细胞分化。细胞增殖-毒性检测试剂盒法检测细胞活性；流式细胞术检测细胞凋亡、周期，并联合细胞免疫荧光检测PGC-1β表达；抗酒石酸酸性磷酸酶（TRAP）染色鉴定成熟破骨细胞；甲苯胺蓝染色评估骨吸收陷窝面积。核浆分离联合蛋白质免疫印迹法检测PGC-1β表达及定位、破骨细胞相关蛋白[树突状细胞特异性跨膜蛋白（DC-STAMP）、组织蛋白酶K（cathepsin K）、TRAP和基质金属蛋白酶-9（MMP-9）表达及非经典核因子（NF）-κB通路关键分子NF-κB诱导激酶（NIK）和转录因子RelB的水平。 结果： PGC-1β主要表达在RAW264.7细胞胞核中。下调或过表达PGC-1β不影响细胞活性、凋亡及周期。下调PGC-1β后成熟破骨细胞数（49个±21个比147个±42个，P=0.004）及骨吸收陷凹面积（42.11μm(2)±11.30 μm(2)比204.80μm(2)±31.09 μm(2)，P<0.001）较其对照组显著减少，cathepsin K、TRAP及MMP-9表达下降，而DC-STAMP无明显变化。过表达PGC-1β促进破骨细胞分化活化及cathepsin K、TRAP、MMP-9表达，下调PGC-1β抑制RAW264.7细胞胞浆NIK和胞核RelB表达。 结论： PGC-1β可激活非经典NF-κB通路NIK/RelB促进RAW264.7细胞向破骨细胞分化及促进骨吸收功能，有望成为治疗骨质疏松症的新靶点。." @default.
• W2997392911 created "2020-01-10" @default.
• W2997392911 creator A5002842847 @default.
• W2997392911 creator A5005506987 @default.
• W2997392911 creator A5006750063 @default.
• W2997392911 creator A5019095151 @default.
• W2997392911 creator A5050600410 @default.
• W2997392911 creator A5052524433 @default.
• W2997392911 creator A5065698665 @default.
• W2997392911 creator A5087895434 @default.
• W2997392911 date "2019-12-10" @default.
• W2997392911 modified "2023-09-26" @default.
• W2997392911 title "[Potential mechanism of transcription factor peroxisome proliferator-activated receptor-gamma coactivator-1 beta on promoting osteoclastogenesis]." @default.
• W2997392911 doi "https://doi.org/10.3760/cma.j.issn.0376-2491.2019.46.009" @default.
• W2997392911 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/31826586" @default.
• W2997392911 hasPublicationYear "2019" @default.
• W2997392911 type Work @default.
• W2997392911 sameAs 2997392911 @default.
• W2997392911 citedByCount "0" @default.
• W2997392911 crossrefType "journal-article" @default.
• W2997392911 hasAuthorship W2997392911A5002842847 @default.
• W2997392911 hasAuthorship W2997392911A5005506987 @default.
• W2997392911 hasAuthorship W2997392911A5006750063 @default.
• W2997392911 hasAuthorship W2997392911A5019095151 @default.
• W2997392911 hasAuthorship W2997392911A5050600410 @default.
• W2997392911 hasAuthorship W2997392911A5052524433 @default.
• W2997392911 hasAuthorship W2997392911A5065698665 @default.
• W2997392911 hasAuthorship W2997392911A5087895434 @default.
• W2997392911 hasConcept C101990758 @default.
• W2997392911 hasConcept C1491633281 @default.
• W2997392911 hasConcept C153911025 @default.
• W2997392911 hasConcept C170493617 @default.
• W2997392911 hasConcept C185592680 @default.
• W2997392911 hasConcept C2776033226 @default.
• W2997392911 hasConcept C2779428903 @default.
• W2997392911 hasConcept C29537977 @default.
• W2997392911 hasConcept C53227056 @default.
• W2997392911 hasConcept C55493867 @default.
• W2997392911 hasConcept C86803240 @default.
• W2997392911 hasConcept C88045685 @default.
• W2997392911 hasConcept C95444343 @default.
• W2997392911 hasConceptScore W2997392911C101990758 @default.
• W2997392911 hasConceptScore W2997392911C1491633281 @default.
• W2997392911 hasConceptScore W2997392911C153911025 @default.
• W2997392911 hasConceptScore W2997392911C170493617 @default.
• W2997392911 hasConceptScore W2997392911C185592680 @default.
• W2997392911 hasConceptScore W2997392911C2776033226 @default.
• W2997392911 hasConceptScore W2997392911C2779428903 @default.
• W2997392911 hasConceptScore W2997392911C29537977 @default.
• W2997392911 hasConceptScore W2997392911C53227056 @default.
• W2997392911 hasConceptScore W2997392911C55493867 @default.
• W2997392911 hasConceptScore W2997392911C86803240 @default.
• W2997392911 hasConceptScore W2997392911C88045685 @default.
• W2997392911 hasConceptScore W2997392911C95444343 @default.
• W2997392911 hasIssue "46" @default.
• W2997392911 hasLocation W29973929111 @default.
• W2997392911 hasOpenAccess W2997392911 @default.
• W2997392911 hasPrimaryLocation W29973929111 @default.
• W2997392911 hasRelatedWork W2028593879 @default.
• W2997392911 hasRelatedWork W2030906523 @default.
• W2997392911 hasRelatedWork W2038060585 @default.
• W2997392911 hasRelatedWork W2121823387 @default.
• W2997392911 hasRelatedWork W2142016853 @default.
• W2997392911 hasRelatedWork W2174832515 @default.
• W2997392911 hasRelatedWork W2398143245 @default.
• W2997392911 hasRelatedWork W2618691574 @default.
• W2997392911 hasRelatedWork W2990955505 @default.
• W2997392911 hasRelatedWork W3045290096 @default.
• W2997392911 hasVolume "99" @default.
• W2997392911 isParatext "false" @default.
• W2997392911 isRetracted "false" @default.
• W2997392911 magId "2997392911" @default.
• W2997392911 workType "article" @default.