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- W2997514326 endingPage "33" @default.
- W2997514326 startingPage "33" @default.
- W2997514326 abstract "Streptococcus (S.) suis is a major cause of economic losses in the pig industry worldwide and is an emerging zoonotic pathogen. One important virulence-associated factor is suilysin (SLY), a toxin that belongs to the family of cholesterol-dependent pore-forming cytolysins (CDC). However, the precise role of SLY in host–pathogen interactions is still unclear. Here, we investigated the susceptibility of different respiratory epithelial cells to SLY, including immortalized cell lines (HEp-2 and NPTr cells), which are frequently used in in vitro studies on S. suis virulence mechanisms, as well as primary porcine respiratory cells, which represent the first line of barrier during S. suis infections. SLY-induced cell damage was determined by measuring the release of lactate dehydrogenase after infection with a virulent S. suis serotype 2 strain, its isogenic SLY-deficient mutant strain, or treatment with the recombinant protein. HEp-2 cells were most susceptible, whereas primary epithelial cells were hardly affected by the toxin. This prompted us to study possible explanations for these differences. We first investigated the binding capacity of SLY using flow cytometry analysis. Since binding and pore-formation of CDC is dependent on the membrane composition, we also determined the cellular cholesterol content of the different cell types using TLC and HPLC. Finally, we examined the ability of those cells to reseal SLY-induced pores using flow cytometry analysis. Our results indicated that the amount of membrane-bound SLY, the cholesterol content of the cells, as well as their resealing capacity all affect the susceptibility of the different cells regarding the effects of SLY. These findings underline the differences of in vitro pathogenicity models and may further help to dissect the biological role of SLY during S. suis infections." @default.
- W2997514326 created "2020-01-10" @default.
- W2997514326 creator A5002038477 @default.
- W2997514326 creator A5010629480 @default.
- W2997514326 creator A5033122295 @default.
- W2997514326 creator A5046715879 @default.
- W2997514326 creator A5060528819 @default.
- W2997514326 date "2019-12-30" @default.
- W2997514326 modified "2023-10-18" @default.
- W2997514326 title "Membrane Binding, Cellular Cholesterol Content and Resealing Capacity Contribute to Epithelial Cell Damage Induced by Suilysin of Streptococcus suis" @default.
- W2997514326 cites W1564370956 @default.
- W2997514326 cites W1567281461 @default.
- W2997514326 cites W1585727595 @default.
- W2997514326 cites W1682958912 @default.
- W2997514326 cites W1815452743 @default.
- W2997514326 cites W1882240799 @default.
- W2997514326 cites W1964185962 @default.
- W2997514326 cites W1966294953 @default.
- W2997514326 cites W1968155437 @default.
- W2997514326 cites W1970973269 @default.
- W2997514326 cites W1971260722 @default.
- W2997514326 cites W1974164851 @default.
- W2997514326 cites W1975611355 @default.
- W2997514326 cites W1993747952 @default.
- W2997514326 cites W2001599041 @default.
- W2997514326 cites W2002009881 @default.
- W2997514326 cites W2002608721 @default.
- W2997514326 cites W2007395394 @default.
- W2997514326 cites W2008703399 @default.
- W2997514326 cites W2013192847 @default.
- W2997514326 cites W2014054827 @default.
- W2997514326 cites W2014079110 @default.
- W2997514326 cites W2024790457 @default.
- W2997514326 cites W2024791084 @default.
- W2997514326 cites W2025104380 @default.
- W2997514326 cites W2026216653 @default.
- W2997514326 cites W2027053173 @default.
- W2997514326 cites W2029248129 @default.
- W2997514326 cites W2033271933 @default.
- W2997514326 cites W2038666785 @default.
- W2997514326 cites W2039151590 @default.
- W2997514326 cites W2039782193 @default.
- W2997514326 cites W2040687198 @default.
- W2997514326 cites W2041629274 @default.
- W2997514326 cites W2049827243 @default.
- W2997514326 cites W2050129379 @default.
- W2997514326 cites W2051826275 @default.
- W2997514326 cites W2055272492 @default.
- W2997514326 cites W2056297389 @default.
- W2997514326 cites W2056923102 @default.
- W2997514326 cites W2057417107 @default.
- W2997514326 cites W2058482910 @default.
- W2997514326 cites W2062699400 @default.
- W2997514326 cites W2063438040 @default.
- W2997514326 cites W2065542491 @default.
- W2997514326 cites W2065968830 @default.
- W2997514326 cites W2079379896 @default.
- W2997514326 cites W2081359143 @default.
- W2997514326 cites W2082887439 @default.
- W2997514326 cites W2087514908 @default.
- W2997514326 cites W2087901582 @default.
- W2997514326 cites W2089579511 @default.
- W2997514326 cites W2091809942 @default.
- W2997514326 cites W2095097063 @default.
- W2997514326 cites W2096515501 @default.
- W2997514326 cites W2098433114 @default.
- W2997514326 cites W2105094451 @default.
- W2997514326 cites W2105430590 @default.
- W2997514326 cites W2112248316 @default.
- W2997514326 cites W2116486895 @default.
- W2997514326 cites W2119866943 @default.
- W2997514326 cites W2121877228 @default.
- W2997514326 cites W2122430143 @default.
- W2997514326 cites W2123109660 @default.
- W2997514326 cites W2129463316 @default.
- W2997514326 cites W2134183923 @default.
- W2997514326 cites W2135958599 @default.
- W2997514326 cites W2136996256 @default.
- W2997514326 cites W2140542375 @default.
- W2997514326 cites W2140699188 @default.
- W2997514326 cites W2142427836 @default.
- W2997514326 cites W2142847489 @default.
- W2997514326 cites W2144754434 @default.
- W2997514326 cites W2148150786 @default.
- W2997514326 cites W2149070070 @default.
- W2997514326 cites W2149696244 @default.
- W2997514326 cites W2152311269 @default.
- W2997514326 cites W2153704724 @default.
- W2997514326 cites W2154167687 @default.
- W2997514326 cites W2156737579 @default.
- W2997514326 cites W2160293301 @default.
- W2997514326 cites W2163246095 @default.
- W2997514326 cites W2165019431 @default.
- W2997514326 cites W2300552860 @default.
- W2997514326 cites W2397218655 @default.
- W2997514326 cites W2460292404 @default.
- W2997514326 cites W2479159856 @default.
- W2997514326 cites W2587303037 @default.