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• W2997545102 abstract "Cardiovascular dysfunction often occurs after high-level spinal cord injury. Disrupting supraspinal vasomotor pathways affects basal hemodynamics and contributes to the development of autonomic dysreflexia (AD). Transplantation of early-stage neurons to the injured cord may reconstruct the descending projections to enhance cardiovascular performance. To determine the specific role of reestablishing serotonergic regulation of hemodynamics, we implanted serotonergic (5-HT + ) neuron-enriched embryonic raphe nucleus-derived neural stem cells/progenitors (RN-NSCs) into a complete spinal cord transection lesion site in adult female rats. Grafting embryonic spinal cord-derived NSCs or injury alone served as 2 controls. Ten weeks after injury/grafting, histological analysis revealed well-survived grafts and partial integration with host tissues in the lesion site. Numerous graft-derived serotonergic axons topographically projected to the caudal autonomic regions. Neuronal tracing showed that host supraspinal vasomotor pathways regenerated into the graft, and 5-HT + neurons within graft and host brainstem neurons were transsynaptically labeled by injecting pseudorabies virus (PRV-614) into the kidney, indicating reconnected serotonergic circuits regulating autonomic activity. Using an implanted telemeter to record cardiovascular parameters, grafting RN-NSCs restored resting mean arterial pressure to normal levels and remarkably alleviated naturally occurring and colorectal distension-induced AD. Subsequent pharmacological blockade of 5-HT 2A receptors with ketanserin in RN-NSC-grafted rats reduced resting mean arterial pressure and increased heart rate in all but 2 controls. Furthermore, spinal cord retransection below RN-NSC grafts partially eliminated the recovery in AD. Collectively, these data indicate that RN-NSCs grafted into a spinal cord injury site relay supraspinal control of serotonergic regulation for sympathetic activity to improve cardiovascular function. SIGNIFICANCE STATEMENT Disruption of supraspinal vasomotor pathways results in cardiovascular dysfunction following high-level spinal cord injury. To reestablish the descending regulation of autonomic function, we transplanted serotonergic neuron enriched embryonic raphe nucleus-derived neural stem cells/progenitors into the lesion site of completely transected rat spinal cord. Consequently, grafted raphe nucleus-derived neural stem cells/progenitors acted as a neuronal relay to reconnect supraspinal center and spinal sympathetic neurons below the injury. The reconstituted serotonergic regulation of sympathetic activity led to the improvement of hemodynamic parameters and mitigated autonomic dysreflexia. Based on morphological and physiological results, this study validates the effectiveness of transplanting early-stage serotonergic neurons into the spinal cord for cardiovascular functional recovery after spinal cord injury." @default.
• W2997545102 created "2020-01-10" @default.
• W2997545102 creator A5008690586 @default.
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• W2997545102 date "2020-01-02" @default.
• W2997545102 modified "2023-10-17" @default.
• W2997545102 title "Grafting Embryonic Raphe Neurons Reestablishes Serotonergic Regulation of Sympathetic Activity to Improve Cardiovascular Function after Spinal Cord Injury" @default.
• W2997545102 cites W1533494462 @default.
• W2997545102 cites W1537549309 @default.
• W2997545102 cites W1829036625 @default.
• W2997545102 cites W1877949834 @default.
• W2997545102 cites W1944007413 @default.
• W2997545102 cites W1964583098 @default.
• W2997545102 cites W1969444386 @default.
• W2997545102 cites W1970976264 @default.
• W2997545102 cites W1972078681 @default.
• W2997545102 cites W1974432822 @default.
• W2997545102 cites W1977664716 @default.
• W2997545102 cites W1979725545 @default.
• W2997545102 cites W1987184086 @default.
• W2997545102 cites W1990277219 @default.
• W2997545102 cites W1997768217 @default.
• W2997545102 cites W1998753032 @default.
• W2997545102 cites W2000380515 @default.
• W2997545102 cites W2004017939 @default.
• W2997545102 cites W2008810977 @default.
• W2997545102 cites W2012253856 @default.
• W2997545102 cites W2013033561 @default.
• W2997545102 cites W2016181711 @default.
• W2997545102 cites W2020410426 @default.
• W2997545102 cites W2023310418 @default.
• W2997545102 cites W2024927923 @default.
• W2997545102 cites W2026673645 @default.
• W2997545102 cites W2027030301 @default.
• W2997545102 cites W2031837182 @default.
• W2997545102 cites W2037982276 @default.
• W2997545102 cites W2038313975 @default.
• W2997545102 cites W2051592757 @default.
• W2997545102 cites W2056880939 @default.
• W2997545102 cites W2060844965 @default.
• W2997545102 cites W2061102206 @default.
• W2997545102 cites W2061189955 @default.
• W2997545102 cites W2064996151 @default.
• W2997545102 cites W2065190958 @default.
• W2997545102 cites W2067253901 @default.
• W2997545102 cites W207311083 @default.
• W2997545102 cites W2081502532 @default.
• W2997545102 cites W2083608759 @default.
• W2997545102 cites W2086524344 @default.
• W2997545102 cites W2090758376 @default.
• W2997545102 cites W2092667479 @default.
• W2997545102 cites W2095326468 @default.
• W2997545102 cites W2107352769 @default.
• W2997545102 cites W2111953197 @default.
• W2997545102 cites W2115992630 @default.
• W2997545102 cites W2118406111 @default.
• W2997545102 cites W2130153447 @default.
• W2997545102 cites W2136723454 @default.
• W2997545102 cites W2137590454 @default.
• W2997545102 cites W2153286899 @default.
• W2997545102 cites W2154545174 @default.
• W2997545102 cites W2156757115 @default.
• W2997545102 cites W2157602413 @default.
• W2997545102 cites W2171416399 @default.
• W2997545102 cites W2181057792 @default.
• W2997545102 cites W2271908519 @default.
• W2997545102 cites W2275984207 @default.
• W2997545102 cites W2329818534 @default.
• W2997545102 cites W2405365757 @default.
• W2997545102 cites W2608611322 @default.
• W2997545102 cites W2611992885 @default.
• W2997545102 cites W2748049166 @default.
• W2997545102 cites W2750359605 @default.
• W2997545102 cites W2757871024 @default.
• W2997545102 cites W2773600389 @default.
• W2997545102 cites W2796285104 @default.
• W2997545102 cites W2835108475 @default.
• W2997545102 cites W2898442268 @default.
• W2997545102 cites W2990424700 @default.
• W2997545102 cites W4242740922 @default.
• W2997545102 cites W4243913623 @default.
• W2997545102 doi "https://doi.org/10.1523/jneurosci.1654-19.2019" @default.
• W2997545102 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/7002146" @default.
• W2997545102 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/31896670" @default.
• W2997545102 hasPublicationYear "2020" @default.
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