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• W2997771325 endingPage "465" @default.
• W2997771325 startingPage "456" @default.
• W2997771325 abstract "Lipase is an enzyme that catalyzes the hydrolysis of lipids in watery and organic interactions they can catalyze synthetic reactions, involving inter esterification among triglycerides and alcohols to produce glycerin and fatty acids. The recovery of soluble lipase is not possible which makes this assay method expensive. The current study efforts on the development of the stability of a lipase B Candida antarctica as Cross-Linked Enzyme Aggregates (CLEAs). Then the graphene oxide nanoparticles (GO-MNPs) were prepared, different conditions such as temperature, pH, storage stability, reusability, and organic solvents were used to compare the free lipase and lipase-GO-MNPs-CLEAs activities. Different techniques were used to analyze the preparation compounds. The saturated ammonium sulfate (55%) and cross-linked with 125 mM glutaraldehyde for 10 h at 4 °C were found to optimization the synthesis of lipase-GO-MNPs-CLEAs with the maximum activity recovery of 96.5%. Storage stability significantly developed for the lipase-GO-MNPs-CLEAs comparing to the free lipase. The current study for the first time modified the MNPs by using hyaluronic acid (HA) which improved the stability and nano-biocatalytic performance in conditions into the solvent and temperature which will have a significant influence on the improvement of bio catalytic systems in the industrial applications." @default.
• W2997771325 created "2020-01-10" @default.
• W2997771325 creator A5071268036 @default.
• W2997771325 date "2020-02-01" @default.
• W2997771325 modified "2023-10-16" @default.
• W2997771325 title "Lipase immobilization on synthesized hyaluronic acid-coated magnetic nanoparticle-functionalized graphene oxide composites as new biocatalysts: Improved reusability, stability, and activity" @default.
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• W2997771325 doi "https://doi.org/10.1016/j.ijbiomac.2019.12.233" @default.
• W2997771325 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/31883900" @default.
• W2997771325 hasPublicationYear "2020" @default.
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