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• W2997925976 endingPage "2489" @default.
• W2997925976 startingPage "2471" @default.
• W2997925976 abstract "Intrinsically disordered proteins (IDPs) or regions of intrinsic disorder in otherwise folded proteins (IDRs) play important roles in many different biological processes, including formation of biological condensates via liquid-liquid phase separation. NMR spectroscopy is a powerful tool for obtaining site-specific structural and dynamical information on IDPs/IDRs, and recent efforts have focused on the development of experiments for atomic-resolution studies of these molecules. These include triple-resonance experiments that are based on 13CO-direct detection of magnetization, exploiting increased sensitivity of cryogenically cooled probes. In order to evaluate the different classes of experiment for studies of IDRs or IDPs in both dilute and phase-separated environments, in particular at neutral and higher pHs where many of these proteins phase separate, we compared 13CO-detect versus 1Hα-detect experiments, showing that significant sensitivity gains are achieved via proton detection under the conditions of our experiments. A suite of 1Hα-detect experiments was subsequently developed for studies of IDPs/IDRs and applied to the dilute phase of a 103-residue disordered region of CAPRIN1 that phase separates at neutral pH. Residue-specific chemical shifts derived from our study enable the accurate prediction of the importance of the N-terminal Arg-containing region of this construct for promoting phase separation relative to other Arg-rich stretches of sequence, subsequently confirmed by mutagenesis. Our study emphasizes that the sequence positions of key residues can be a critical factor in controlling phase separation and highlights the unique role of NMR in establishing the relations between amino acid sequence and phase-separation propensity." @default.
• W2997925976 created "2020-01-10" @default.
• W2997925976 creator A5000058915 @default.
• W2997925976 creator A5020050875 @default.
• W2997925976 creator A5027862452 @default.
• W2997925976 creator A5057664407 @default.
• W2997925976 creator A5062938069 @default.
• W2997925976 date "2020-01-03" @default.
• W2997925976 modified "2023-09-29" @default.
• W2997925976 title "NMR Experiments for Studies of Dilute and Condensed Protein Phases: Application to the Phase-Separating Protein CAPRIN1" @default.
• W2997925976 cites W1854281301 @default.
• W2997925976 cites W1938908576 @default.
• W2997925976 cites W1964951230 @default.
• W2997925976 cites W1983282283 @default.
• W2997925976 cites W1983770488 @default.
• W2997925976 cites W1984484995 @default.
• W2997925976 cites W1986427666 @default.
• W2997925976 cites W1987352448 @default.
• W2997925976 cites W1989350467 @default.
• W2997925976 cites W1993461145 @default.
• W2997925976 cites W1996562045 @default.
• W2997925976 cites W1997775758 @default.
• W2997925976 cites W1998213446 @default.
• W2997925976 cites W1998776274 @default.
• W2997925976 cites W2003589347 @default.
• W2997925976 cites W2004164815 @default.
• W2997925976 cites W2010969835 @default.
• W2997925976 cites W2012613971 @default.
• W2997925976 cites W2012851941 @default.
• W2997925976 cites W2014349449 @default.
• W2997925976 cites W2023652169 @default.
• W2997925976 cites W2025217587 @default.
• W2997925976 cites W2036352592 @default.
• W2997925976 cites W2036556016 @default.
• W2997925976 cites W2042670295 @default.
• W2997925976 cites W2046825058 @default.
• W2997925976 cites W2052224749 @default.
• W2997925976 cites W2052699134 @default.
• W2997925976 cites W2060770036 @default.
• W2997925976 cites W2060905914 @default.
• W2997925976 cites W2065720885 @default.
• W2997925976 cites W2068227670 @default.
• W2997925976 cites W2073444730 @default.
• W2997925976 cites W2075980942 @default.
• W2997925976 cites W2078591248 @default.
• W2997925976 cites W2078684251 @default.
• W2997925976 cites W2085978470 @default.
• W2997925976 cites W2091797182 @default.
• W2997925976 cites W2092828563 @default.
• W2997925976 cites W2103161618 @default.
• W2997925976 cites W2112103709 @default.
• W2997925976 cites W2113670799 @default.
• W2997925976 cites W2115850718 @default.
• W2997925976 cites W2123727062 @default.
• W2997925976 cites W2125159941 @default.
• W2997925976 cites W2127463703 @default.
• W2997925976 cites W2135071373 @default.
• W2997925976 cites W2136219546 @default.
• W2997925976 cites W2139440265 @default.
• W2997925976 cites W2147281292 @default.
• W2997925976 cites W2150860983 @default.
• W2997925976 cites W2154977374 @default.
• W2997925976 cites W2156771958 @default.
• W2997925976 cites W2168639701 @default.
• W2997925976 cites W2169821755 @default.
• W2997925976 cites W2169988510 @default.
• W2997925976 cites W2267482727 @default.
• W2997925976 cites W2289501793 @default.
• W2997925976 cites W2311204218 @default.
• W2997925976 cites W2335345046 @default.
• W2997925976 cites W2554176835 @default.
• W2997925976 cites W2590568317 @default.
• W2997925976 cites W2735988105 @default.
• W2997925976 cites W2755105920 @default.
• W2997925976 cites W2755486970 @default.
• W2997925976 cites W2756821926 @default.
• W2997925976 cites W2767613320 @default.
• W2997925976 cites W2767821809 @default.
• W2997925976 cites W2771032809 @default.
• W2997925976 cites W2782841102 @default.
• W2997925976 cites W2784370070 @default.
• W2997925976 cites W2786469974 @default.
• W2997925976 cites W2790466401 @default.
• W2997925976 cites W2793078357 @default.
• W2997925976 cites W2808121690 @default.
• W2997925976 cites W2810866092 @default.
• W2997925976 cites W2900375353 @default.
• W2997925976 cites W2907119067 @default.
• W2997925976 cites W2914590607 @default.
• W2997925976 cites W2937989193 @default.
• W2997925976 cites W2946724232 @default.
• W2997925976 cites W2949078846 @default.
• W2997925976 cites W2969743565 @default.
• W2997925976 cites W30334872 @default.
• W2997925976 cites W4236258478 @default.
• W2997925976 cites W4236396377 @default.
• W2997925976 cites W4253049361 @default.
• W2997925976 doi "https://doi.org/10.1021/jacs.9b12208" @default.

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