Matches in SemOpenAlex for { <https://semopenalex.org/work/W2998223589> ?p ?o ?g. }
- W2998223589 endingPage "117244" @default.
- W2998223589 startingPage "117244" @default.
- W2998223589 abstract "Endplate chondrocyte apoptosis is an important contributor to the pathogenesis of cartilaginous endplate (CEP) degeneration that leads to the initiation and development of intervertebral disc degeneration (IDD). In this study, we hypothesized that Parkin-mediated mitophagy and nuclear factor E2-related factor 2 (Nrf2)-mediated antioxidant system played an important role in endplate chondrocyte survival under pathological conditions. Human endplate chondrocytes were stimulated with H2O2 to mimic pathological conditions. Western blotting, immunofluorescence staining, and flow cytometry were applied to detect the indicators related to mitochondrial dynamics, mitophagy, Nrf2 signaling, and apoptosis. The puncture-induced rat models were established to evaluate the changes in vivo. Our results showed that H2O2 induced oxidative stress, mitochondrial dysfunction, and apoptosis in endplate chondrocytes. These H2O2-induced detrimental effects were inhibited by pretreatment with the mitochondria-targeted antioxidant Mito-TEMPO. In addition, mitochondrial dynamics, Parkin-mediated elimination of dysfunctional mitochondria, and Nrf2-mediated antioxidant system were promoted by H2O2. Knockdown of Parkin or Nrf2 increased H2O2-induced detrimental effects. Moreover, upregulation of Parkin and Nrf2 by polydatin protected endplate chondrocytes against H2O2-induced mitochondrial dysfunction, oxidative stress, and apoptosis. Finally, puncture-induced rat models showed that polydatin exerted a protective effect on CEP and disc degeneration. Targeting Parkin and Nrf2 to improve mitochondrial homeostasis, redox balance and endplate chondrocyte survival may represent a potential therapeutic strategy for preventing IDD." @default.
- W2998223589 created "2020-01-10" @default.
- W2998223589 creator A5007241601 @default.
- W2998223589 creator A5027251492 @default.
- W2998223589 creator A5046499103 @default.
- W2998223589 creator A5060978357 @default.
- W2998223589 creator A5068982268 @default.
- W2998223589 creator A5074896161 @default.
- W2998223589 date "2020-02-01" @default.
- W2998223589 modified "2023-10-17" @default.
- W2998223589 title "Parkin and Nrf2 prevent oxidative stress-induced apoptosis in intervertebral endplate chondrocytes via inducing mitophagy and anti-oxidant defenses" @default.
- W2998223589 cites W1926228770 @default.
- W2998223589 cites W1966188170 @default.
- W2998223589 cites W1970323059 @default.
- W2998223589 cites W1989077880 @default.
- W2998223589 cites W2017099154 @default.
- W2998223589 cites W2032083413 @default.
- W2998223589 cites W2058667888 @default.
- W2998223589 cites W2069759916 @default.
- W2998223589 cites W2093009907 @default.
- W2998223589 cites W2106674569 @default.
- W2998223589 cites W2109773179 @default.
- W2998223589 cites W2117800935 @default.
- W2998223589 cites W2122506511 @default.
- W2998223589 cites W2288482594 @default.
- W2998223589 cites W2318077830 @default.
- W2998223589 cites W2562825495 @default.
- W2998223589 cites W2572344066 @default.
- W2998223589 cites W2588981667 @default.
- W2998223589 cites W2589016670 @default.
- W2998223589 cites W2589358108 @default.
- W2998223589 cites W2600292433 @default.
- W2998223589 cites W2617115371 @default.
- W2998223589 cites W2742147437 @default.
- W2998223589 cites W2755978353 @default.
- W2998223589 cites W2771705633 @default.
- W2998223589 cites W2788644497 @default.
- W2998223589 cites W2790410554 @default.
- W2998223589 cites W2791191589 @default.
- W2998223589 cites W2801988573 @default.
- W2998223589 cites W2873284260 @default.
- W2998223589 cites W2885631116 @default.
- W2998223589 cites W2888792815 @default.
- W2998223589 cites W2890376302 @default.
- W2998223589 cites W2890698731 @default.
- W2998223589 cites W2890986406 @default.
- W2998223589 cites W2898195830 @default.
- W2998223589 cites W2899699222 @default.
- W2998223589 cites W2899967263 @default.
- W2998223589 cites W2900765423 @default.
- W2998223589 cites W2911950918 @default.
- W2998223589 cites W2915796707 @default.
- W2998223589 cites W2954937777 @default.
- W2998223589 cites W2961283112 @default.
- W2998223589 cites W2964793689 @default.
- W2998223589 cites W2974938421 @default.
- W2998223589 cites W2981246009 @default.
- W2998223589 cites W344068791 @default.
- W2998223589 doi "https://doi.org/10.1016/j.lfs.2019.117244" @default.
- W2998223589 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/31891721" @default.
- W2998223589 hasPublicationYear "2020" @default.
- W2998223589 type Work @default.
- W2998223589 sameAs 2998223589 @default.
- W2998223589 citedByCount "59" @default.
- W2998223589 countsByYear W29982235892020 @default.
- W2998223589 countsByYear W29982235892021 @default.
- W2998223589 countsByYear W29982235892022 @default.
- W2998223589 countsByYear W29982235892023 @default.
- W2998223589 crossrefType "journal-article" @default.
- W2998223589 hasAuthorship W2998223589A5007241601 @default.
- W2998223589 hasAuthorship W2998223589A5027251492 @default.
- W2998223589 hasAuthorship W2998223589A5046499103 @default.
- W2998223589 hasAuthorship W2998223589A5060978357 @default.
- W2998223589 hasAuthorship W2998223589A5068982268 @default.
- W2998223589 hasAuthorship W2998223589A5074896161 @default.
- W2998223589 hasConcept C142724271 @default.
- W2998223589 hasConcept C185592680 @default.
- W2998223589 hasConcept C190283241 @default.
- W2998223589 hasConcept C202751555 @default.
- W2998223589 hasConcept C203522944 @default.
- W2998223589 hasConcept C2776151105 @default.
- W2998223589 hasConcept C2777668072 @default.
- W2998223589 hasConcept C2779134260 @default.
- W2998223589 hasConcept C2779324830 @default.
- W2998223589 hasConcept C2779734285 @default.
- W2998223589 hasConcept C2781403057 @default.
- W2998223589 hasConcept C28859421 @default.
- W2998223589 hasConcept C48349386 @default.
- W2998223589 hasConcept C55493867 @default.
- W2998223589 hasConcept C71924100 @default.
- W2998223589 hasConcept C86803240 @default.
- W2998223589 hasConcept C95444343 @default.
- W2998223589 hasConceptScore W2998223589C142724271 @default.
- W2998223589 hasConceptScore W2998223589C185592680 @default.
- W2998223589 hasConceptScore W2998223589C190283241 @default.
- W2998223589 hasConceptScore W2998223589C202751555 @default.
- W2998223589 hasConceptScore W2998223589C203522944 @default.
- W2998223589 hasConceptScore W2998223589C2776151105 @default.