FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2998599004> ?p ?o ?g. }

• W2998599004 endingPage "104654" @default.
• W2998599004 startingPage "104654" @default.
• W2998599004 abstract "Akt is one of the most important downstream effectors of phosphatidylinositol 3-kinase/mTOR pathway. Hyperactivation and expression of this pathway are seen in a variety of neurological disorders including human temporal lobe epilepsy with hippocampal sclerosis (TLE-HS). Nevertheless, the expression and activation profiles of the Akt isoforms, Akt1, Akt2, and Akt3 and their functional roles in human TLE-HS have not been studied. We examined the protein expression and activation (phosphorylation) patterns of Akt and its isoforms in human hippocampal tissue from TLE and non-TLE patients. A phosphoproteomic approach followed by interactome analysis of each Akt isoform was used to understand protein-protein interactions and their role in TLE-HS pathology. Our results demonstrated activation of the Akt/mTOR pathway as well as activation of Akt downstream substrates like GSK3β, mTOR, and S6 in TLE-HS samples. Akt1 isoform levels were significantly increased in the TLE-HS samples as compared to the non-TLE samples. Most importantly, different isoforms were activated in different TLE-HS samples, Akt2 was activated in three samples, Akt2 and Akt1 were simultaneously activated in one sample and Akt3 was activated in two samples. Our phosphoproteomic screen across six TLE-HS samples identified 183 proteins phosphorylated by Akt isoforms, 29 of these proteins belong to cytoskeletal modification. Also, we were able to identify proteins of several other classes involved in glycolysis, neuronal development, protein folding and excitatory amino acid transport functions as Akt substrates. Taken together, our data offer clues to understand the role of Akt and its isoforms in underlying the pathology of TLE-HS and further, modulation of Akt/mTOR pathway using Akt isoforms specific inhibitors may offer a new therapeutic window for treatment of human TLE-HS." @default.
• W2998599004 created "2020-01-10" @default.
• W2998599004 creator A5015186426 @default.
• W2998599004 creator A5021632908 @default.
• W2998599004 creator A5026207754 @default.
• W2998599004 creator A5032105516 @default.
• W2998599004 creator A5043405770 @default.
• W2998599004 creator A5043778713 @default.
• W2998599004 creator A5048005961 @default.
• W2998599004 creator A5058047028 @default.
• W2998599004 creator A5058346037 @default.
• W2998599004 creator A5083178646 @default.
• W2998599004 creator A5087505937 @default.
• W2998599004 date "2020-03-01" @default.
• W2998599004 modified "2023-09-25" @default.
• W2998599004 title "Phosphoproteomic analysis reveals Akt isoform-specific regulation of cytoskeleton proteins in human temporal lobe epilepsy with hippocampal sclerosis" @default.
• W2998599004 cites W1518505396 @default.
• W2998599004 cites W1550524407 @default.
• W2998599004 cites W1558013239 @default.
• W2998599004 cites W1562551442 @default.
• W2998599004 cites W1571461566 @default.
• W2998599004 cites W1776249833 @default.
• W2998599004 cites W1875732734 @default.
• W2998599004 cites W1963770237 @default.
• W2998599004 cites W1967024359 @default.
• W2998599004 cites W1968151197 @default.
• W2998599004 cites W1969713114 @default.
• W2998599004 cites W1970519691 @default.
• W2998599004 cites W1976857838 @default.
• W2998599004 cites W1984951395 @default.
• W2998599004 cites W1985013906 @default.
• W2998599004 cites W1985213868 @default.
• W2998599004 cites W1987520241 @default.
• W2998599004 cites W1993130330 @default.
• W2998599004 cites W1995540460 @default.
• W2998599004 cites W1996299534 @default.
• W2998599004 cites W1998879017 @default.
• W2998599004 cites W1999504198 @default.
• W2998599004 cites W2002738962 @default.
• W2998599004 cites W2003907026 @default.
• W2998599004 cites W2006323609 @default.
• W2998599004 cites W2007114641 @default.
• W2998599004 cites W2012493999 @default.
• W2998599004 cites W2014331589 @default.
• W2998599004 cites W2015513895 @default.
• W2998599004 cites W2021175703 @default.
• W2998599004 cites W2027601065 @default.
• W2998599004 cites W2028268047 @default.
• W2998599004 cites W2029499143 @default.
• W2998599004 cites W2031330093 @default.
• W2998599004 cites W2032665292 @default.
• W2998599004 cites W2033804029 @default.
• W2998599004 cites W2035277224 @default.
• W2998599004 cites W2039849406 @default.
• W2998599004 cites W2040143421 @default.
• W2998599004 cites W2048121649 @default.
• W2998599004 cites W2048848497 @default.
• W2998599004 cites W2051561353 @default.
• W2998599004 cites W2052086914 @default.
• W2998599004 cites W2053073281 @default.
• W2998599004 cites W2055394920 @default.
• W2998599004 cites W2057308155 @default.
• W2998599004 cites W2057370954 @default.
• W2998599004 cites W2061006930 @default.
• W2998599004 cites W2062497077 @default.
• W2998599004 cites W2063120457 @default.
• W2998599004 cites W2065279599 @default.
• W2998599004 cites W2065609541 @default.
• W2998599004 cites W2070194921 @default.
• W2998599004 cites W2070960352 @default.
• W2998599004 cites W2073442661 @default.
• W2998599004 cites W2074029332 @default.
• W2998599004 cites W2074539520 @default.
• W2998599004 cites W2077754511 @default.
• W2998599004 cites W2078821200 @default.
• W2998599004 cites W2078895783 @default.
• W2998599004 cites W2079697909 @default.
• W2998599004 cites W2080744747 @default.
• W2998599004 cites W2085001027 @default.
• W2998599004 cites W2085886216 @default.
• W2998599004 cites W2089348773 @default.
• W2998599004 cites W2093155405 @default.
• W2998599004 cites W2098762137 @default.
• W2998599004 cites W2110095330 @default.
• W2998599004 cites W2110204413 @default.
• W2998599004 cites W2110211257 @default.
• W2998599004 cites W2115796979 @default.
• W2998599004 cites W2116394679 @default.
• W2998599004 cites W2119774379 @default.
• W2998599004 cites W2120723414 @default.
• W2998599004 cites W2123467807 @default.
• W2998599004 cites W2123500593 @default.
• W2998599004 cites W2127615831 @default.
• W2998599004 cites W2131840335 @default.
• W2998599004 cites W2137103231 @default.
• W2998599004 cites W2142495092 @default.
• W2998599004 cites W2143027879 @default.
• W2998599004 cites W2145995195 @default.

• next