Matches in SemOpenAlex for { <https://semopenalex.org/work/W2998780345> ?p ?o ?g. }
- W2998780345 endingPage "217" @default.
- W2998780345 startingPage "209" @default.
- W2998780345 abstract "Metabolic engineering aims to achieve high yields of desired products. The most common strategies focus on optimization of metabolic flux distributions. The dynamic activation or inhibition of gene expression through quorum sensing (QS) has been applied to metabolic engineering. In this study, we designed and constructed a series of QS-based bifunctional dynamic switches (QS switches) capable of synchronizing the up-regulation and down-regulation of genes at different times and intervals. The bifunctional QS switches were based on the Esa QS system, because EsaR regulatory proteins can act as transcriptional activator and repressor. The QS switches’ effectiveness and feasibility were verified through fluorescence characterization. Finally, the QS switches were applied to the production of 5-aminolevulinic acid (ALA) and poly-β-hydroxybutyrate (PHB) to solve two key metabolic engineering problems: necessary gene knockout and redirection of metabolic flux. The production of PHB and ALA was increased 6- and 12-fold in Escherichia coli, respectively." @default.
- W2998780345 created "2020-01-23" @default.
- W2998780345 creator A5028881300 @default.
- W2998780345 creator A5054110892 @default.
- W2998780345 creator A5060727622 @default.
- W2998780345 creator A5067975624 @default.
- W2998780345 creator A5077246875 @default.
- W2998780345 creator A5081189623 @default.
- W2998780345 creator A5085470436 @default.
- W2998780345 creator A5090887276 @default.
- W2998780345 date "2020-01-16" @default.
- W2998780345 modified "2023-10-01" @default.
- W2998780345 title "Quorum Sensing-Based Dual-Function Switch and Its Application in Solving Two Key Metabolic Engineering Problems" @default.
- W2998780345 cites W1502461125 @default.
- W2998780345 cites W1514458902 @default.
- W2998780345 cites W1547106545 @default.
- W2998780345 cites W1878915947 @default.
- W2998780345 cites W1943112463 @default.
- W2998780345 cites W1971439989 @default.
- W2998780345 cites W1972473627 @default.
- W2998780345 cites W1996227484 @default.
- W2998780345 cites W1997494984 @default.
- W2998780345 cites W2002990807 @default.
- W2998780345 cites W2019962216 @default.
- W2998780345 cites W2047630852 @default.
- W2998780345 cites W2051065279 @default.
- W2998780345 cites W2061468525 @default.
- W2998780345 cites W2065338303 @default.
- W2998780345 cites W2073265884 @default.
- W2998780345 cites W2077066088 @default.
- W2998780345 cites W2080040554 @default.
- W2998780345 cites W2083878238 @default.
- W2998780345 cites W2088914103 @default.
- W2998780345 cites W2091340251 @default.
- W2998780345 cites W2092122738 @default.
- W2998780345 cites W2094686752 @default.
- W2998780345 cites W2106673630 @default.
- W2998780345 cites W2107277218 @default.
- W2998780345 cites W2125108766 @default.
- W2998780345 cites W2127869220 @default.
- W2998780345 cites W2144720195 @default.
- W2998780345 cites W2161782059 @default.
- W2998780345 cites W2318810578 @default.
- W2998780345 cites W2325907107 @default.
- W2998780345 cites W2560423727 @default.
- W2998780345 cites W2588456700 @default.
- W2998780345 cites W2604214871 @default.
- W2998780345 cites W2767217582 @default.
- W2998780345 cites W2906878682 @default.
- W2998780345 cites W2947685201 @default.
- W2998780345 cites W3006685530 @default.
- W2998780345 doi "https://doi.org/10.1021/acssynbio.9b00290" @default.
- W2998780345 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/31944663" @default.
- W2998780345 hasPublicationYear "2020" @default.
- W2998780345 type Work @default.
- W2998780345 sameAs 2998780345 @default.
- W2998780345 citedByCount "32" @default.
- W2998780345 countsByYear W29987803452020 @default.
- W2998780345 countsByYear W29987803452021 @default.
- W2998780345 countsByYear W29987803452022 @default.
- W2998780345 countsByYear W29987803452023 @default.
- W2998780345 crossrefType "journal-article" @default.
- W2998780345 hasAuthorship W2998780345A5028881300 @default.
- W2998780345 hasAuthorship W2998780345A5054110892 @default.
- W2998780345 hasAuthorship W2998780345A5060727622 @default.
- W2998780345 hasAuthorship W2998780345A5067975624 @default.
- W2998780345 hasAuthorship W2998780345A5077246875 @default.
- W2998780345 hasAuthorship W2998780345A5081189623 @default.
- W2998780345 hasAuthorship W2998780345A5085470436 @default.
- W2998780345 hasAuthorship W2998780345A5090887276 @default.
- W2998780345 hasConcept C104317684 @default.
- W2998780345 hasConcept C118687296 @default.
- W2998780345 hasConcept C14036430 @default.
- W2998780345 hasConcept C150194340 @default.
- W2998780345 hasConcept C152662350 @default.
- W2998780345 hasConcept C158448853 @default.
- W2998780345 hasConcept C161790260 @default.
- W2998780345 hasConcept C162932704 @default.
- W2998780345 hasConcept C178790620 @default.
- W2998780345 hasConcept C185592680 @default.
- W2998780345 hasConcept C191908910 @default.
- W2998780345 hasConcept C2780443040 @default.
- W2998780345 hasConcept C41008148 @default.
- W2998780345 hasConcept C547475151 @default.
- W2998780345 hasConcept C55493867 @default.
- W2998780345 hasConcept C60987743 @default.
- W2998780345 hasConcept C6350086 @default.
- W2998780345 hasConcept C68709404 @default.
- W2998780345 hasConcept C70721500 @default.
- W2998780345 hasConcept C761482 @default.
- W2998780345 hasConcept C76155785 @default.
- W2998780345 hasConcept C86803240 @default.
- W2998780345 hasConcept C95444343 @default.
- W2998780345 hasConceptScore W2998780345C104317684 @default.
- W2998780345 hasConceptScore W2998780345C118687296 @default.
- W2998780345 hasConceptScore W2998780345C14036430 @default.
- W2998780345 hasConceptScore W2998780345C150194340 @default.
- W2998780345 hasConceptScore W2998780345C152662350 @default.