FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2998795330> ?p ?o ?g. }

• W2998795330 endingPage "1633" @default.
• W2998795330 startingPage "1625" @default.
• W2998795330 abstract "Multidrug membrane transporters can extrude a wide range of substrates, which cause multidrug resistance and ineffective treatment of diseases. In this study, we used three different sized antibiotic drug nanocarriers to study their size-dependent inhibitory effects against Bacillus subtilis. We functionalized 2.4 ± 0.7, 13.0 ± 3.1, and 92.6 ± 4.4 nm silver nanoparticles (Ag NPs) with a monolayer of 11-amino-1-undecanethiol and covalently linked them with antibiotics (ofloxacin, Oflx). The labeling ratios of antibiotics with NPs are 8.6 × 102, 9.4 × 103, and 6.5 × 105 Oflx molecules per NP, respectively. We designed cell culture medium in which both BmrA and ΔBmrA cells grew and functioned normally while ensuring the stabilities of nanocarriers (nonaggregation). These approaches allow us to quantitatively study the dependence of their inhibitory effect against two isogenic strains of B. subtilis, WT (normal expression of BmrA) and ΔBmrA (deletion of bmrA), upon the NP size, antibiotic dose, and BmrA expression. Our results show that the inhibitory effects of nanocarriers highly depend on NP size and antibiotic dose. The same amount of Oflx on 2.4 ± 0.7, 13.0 ± 3.1, and 92.6 ± 4.4 nm nanocarriers shows the 3× lower, nearly the same, and 10× higher inhibitory effects than that of free Oflx, against both WT and ΔBmrA, respectively. Control experiments of the respective sized AgMUNH2 NPs (absence of Oflx) show insignificant inhibitory effects toward both strains. Taken together, the results show multiple factors, such as labeling ratios, multivalent effects, and pharmacodynamics (Oflx localization and distribution), which might play the roles in the size-dependent inhibitory effects on the growth of both WT and ΔBmrA strains. Interestingly, the inhibitory effects of nanocarriers are independent of the expression of BmrA, which could be attributed to the higher efflux of nanocarriers by other membrane transporters in both strains." @default.
• W2998795330 created "2020-01-23" @default.
• W2998795330 creator A5003713629 @default.
• W2998795330 creator A5013737207 @default.
• W2998795330 creator A5032789956 @default.
• W2998795330 creator A5057563515 @default.
• W2998795330 creator A5082696857 @default.
• W2998795330 date "2020-01-13" @default.
• W2998795330 modified "2023-10-10" @default.
• W2998795330 title "Antibiotic Drug Nanocarriers for Probing of Multidrug ABC Membrane Transporter of <i>Bacillus subtilis</i>" @default.
• W2998795330 cites W1570418732 @default.
• W2998795330 cites W1922793792 @default.
• W2998795330 cites W1927723646 @default.
• W2998795330 cites W1965799368 @default.
• W2998795330 cites W1970687129 @default.
• W2998795330 cites W1978360271 @default.
• W2998795330 cites W1978612710 @default.
• W2998795330 cites W1978870288 @default.
• W2998795330 cites W1980179932 @default.
• W2998795330 cites W1981736757 @default.
• W2998795330 cites W1981871034 @default.
• W2998795330 cites W1982033708 @default.
• W2998795330 cites W1986401262 @default.
• W2998795330 cites W1987641460 @default.
• W2998795330 cites W1993852680 @default.
• W2998795330 cites W1994021560 @default.
• W2998795330 cites W1994348363 @default.
• W2998795330 cites W2002531859 @default.
• W2998795330 cites W2003562401 @default.
• W2998795330 cites W2016473957 @default.
• W2998795330 cites W2020260512 @default.
• W2998795330 cites W2020496125 @default.
• W2998795330 cites W2020638819 @default.
• W2998795330 cites W2023218348 @default.
• W2998795330 cites W2030224465 @default.
• W2998795330 cites W2031061254 @default.
• W2998795330 cites W2048100822 @default.
• W2998795330 cites W2064488456 @default.
• W2998795330 cites W2068445631 @default.
• W2998795330 cites W2076189768 @default.
• W2998795330 cites W2076261029 @default.
• W2998795330 cites W2076664843 @default.
• W2998795330 cites W2091525709 @default.
• W2998795330 cites W2094733233 @default.
• W2998795330 cites W2109717868 @default.
• W2998795330 cites W2123474521 @default.
• W2998795330 cites W2135228046 @default.
• W2998795330 cites W2142785321 @default.
• W2998795330 cites W2147018428 @default.
• W2998795330 cites W2160935755 @default.
• W2998795330 cites W2161983628 @default.
• W2998795330 cites W2162097287 @default.
• W2998795330 cites W2168070336 @default.
• W2998795330 cites W2171050584 @default.
• W2998795330 cites W2308408790 @default.
• W2998795330 cites W2317414454 @default.
• W2998795330 cites W2342691654 @default.
• W2998795330 cites W2407380740 @default.
• W2998795330 cites W2414497324 @default.
• W2998795330 cites W258657151 @default.
• W2998795330 cites W2597231659 @default.
• W2998795330 cites W2605748055 @default.
• W2998795330 cites W2605774152 @default.
• W2998795330 cites W2785597087 @default.
• W2998795330 cites W2786179034 @default.
• W2998795330 cites W2796552195 @default.
• W2998795330 cites W2797246261 @default.
• W2998795330 cites W2808711858 @default.
• W2998795330 cites W2887030288 @default.
• W2998795330 cites W2921749090 @default.
• W2998795330 cites W2972830711 @default.
• W2998795330 doi "https://doi.org/10.1021/acsomega.9b03698" @default.
• W2998795330 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/6990642" @default.
• W2998795330 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/32010837" @default.
• W2998795330 hasPublicationYear "2020" @default.
• W2998795330 type Work @default.
• W2998795330 sameAs 2998795330 @default.
• W2998795330 citedByCount "10" @default.
• W2998795330 countsByYear W29987953302020 @default.
• W2998795330 countsByYear W29987953302021 @default.
• W2998795330 countsByYear W29987953302022 @default.
• W2998795330 countsByYear W29987953302023 @default.
• W2998795330 crossrefType "journal-article" @default.
• W2998795330 hasAuthorship W2998795330A5003713629 @default.
• W2998795330 hasAuthorship W2998795330A5013737207 @default.
• W2998795330 hasAuthorship W2998795330A5032789956 @default.
• W2998795330 hasAuthorship W2998795330A5057563515 @default.
• W2998795330 hasAuthorship W2998795330A5082696857 @default.
• W2998795330 hasBestOaLocation W29987953301 @default.
• W2998795330 hasConcept C133936738 @default.
• W2998795330 hasConcept C169760540 @default.
• W2998795330 hasConcept C17077164 @default.
• W2998795330 hasConcept C176947019 @default.
• W2998795330 hasConcept C18150654 @default.
• W2998795330 hasConcept C185592680 @default.
• W2998795330 hasConcept C2777272437 @default.
• W2998795330 hasConcept C2778049240 @default.
• W2998795330 hasConcept C2778512257 @default.

• next