Matches in SemOpenAlex for { <https://semopenalex.org/work/W2998991168> ?p ?o ?g. }
- W2998991168 abstract "Abstract Synaptic adhesion molecules play an important role in the formation, maintenance and refinement of neuronal connectivity. Recently, several leucine rich repeat (LRR) domain containing neuronal adhesion molecules have been characterized including netrin G-ligands, SLITRKs and the synaptic adhesion-like molecules (SALMs). Dysregulation of these adhesion molecules have been genetically and functionally linked to various neurological disorders. Here we investigated the molecular structure and mechanism of ligand interactions for the postsynaptic SALM3 adhesion protein with its presynaptic ligand, receptor protein tyrosine phosphatase σ (PTPσ). We solved the crystal structure of the dimerized leucine rich repeat (LRR) domain of SALM3, revealing the conserved structural features and mechanism of dimerization. Furthermore, we determined the complex structure of SALM3 with PTPσ using small angle X-ray scattering, revealing a 2:2 complex similar to that observed for SALM5. Solution studies unraveled additional flexibility for the complex structure, but validated the uniform mode of action for SALM3 and SALM5 to promote synapse formation. The relevance of the key interface residues was further confirmed by mutational analysis with cellular binding assays and artificial synapse formation assays. Collectively, our results suggest that SALM3 dimerization is a pre-requisite for the SALM3-PTPσ complex to exert synaptogenic activity." @default.
- W2998991168 created "2020-01-23" @default.
- W2998991168 creator A5032900127 @default.
- W2998991168 creator A5033759392 @default.
- W2998991168 creator A5040738906 @default.
- W2998991168 creator A5056663780 @default.
- W2998991168 creator A5063359584 @default.
- W2998991168 date "2020-01-09" @default.
- W2998991168 modified "2023-10-16" @default.
- W2998991168 title "Structural basis of SALM3 dimerization and synaptic adhesion complex formation with PTPσ" @default.
- W2998991168 cites W1591227812 @default.
- W2998991168 cites W1693745137 @default.
- W2998991168 cites W1978695468 @default.
- W2998991168 cites W2003954725 @default.
- W2998991168 cites W2022802536 @default.
- W2998991168 cites W2036931150 @default.
- W2998991168 cites W2037966393 @default.
- W2998991168 cites W2046541204 @default.
- W2998991168 cites W2067561211 @default.
- W2998991168 cites W2068648670 @default.
- W2998991168 cites W2069261633 @default.
- W2998991168 cites W2069299387 @default.
- W2998991168 cites W2072674961 @default.
- W2998991168 cites W2073578218 @default.
- W2998991168 cites W2076977967 @default.
- W2998991168 cites W2077255131 @default.
- W2998991168 cites W2080324921 @default.
- W2998991168 cites W2127774996 @default.
- W2998991168 cites W2128681952 @default.
- W2998991168 cites W2134984452 @default.
- W2998991168 cites W2144081223 @default.
- W2998991168 cites W2146526204 @default.
- W2998991168 cites W2155682217 @default.
- W2998991168 cites W2159211495 @default.
- W2998991168 cites W2159614853 @default.
- W2998991168 cites W2161176515 @default.
- W2998991168 cites W2163341755 @default.
- W2998991168 cites W2164361934 @default.
- W2998991168 cites W2244257221 @default.
- W2998991168 cites W2344383442 @default.
- W2998991168 cites W2376573086 @default.
- W2998991168 cites W2395296394 @default.
- W2998991168 cites W2414311781 @default.
- W2998991168 cites W2502560377 @default.
- W2998991168 cites W2626165253 @default.
- W2998991168 cites W2783071777 @default.
- W2998991168 cites W2783190507 @default.
- W2998991168 cites W2786390877 @default.
- W2998991168 cites W2890270224 @default.
- W2998991168 cites W2893019408 @default.
- W2998991168 cites W2895997008 @default.
- W2998991168 cites W2951581560 @default.
- W2998991168 cites W2975556747 @default.
- W2998991168 cites W2999109014 @default.
- W2998991168 cites W650283456 @default.
- W2998991168 doi "https://doi.org/10.1101/2020.01.09.893701" @default.
- W2998991168 hasPublicationYear "2020" @default.
- W2998991168 type Work @default.
- W2998991168 sameAs 2998991168 @default.
- W2998991168 citedByCount "0" @default.
- W2998991168 crossrefType "posted-content" @default.
- W2998991168 hasAuthorship W2998991168A5032900127 @default.
- W2998991168 hasAuthorship W2998991168A5033759392 @default.
- W2998991168 hasAuthorship W2998991168A5040738906 @default.
- W2998991168 hasAuthorship W2998991168A5056663780 @default.
- W2998991168 hasAuthorship W2998991168A5063359584 @default.
- W2998991168 hasBestOaLocation W29989911681 @default.
- W2998991168 hasConcept C116569031 @default.
- W2998991168 hasConcept C12554922 @default.
- W2998991168 hasConcept C127445978 @default.
- W2998991168 hasConcept C16224149 @default.
- W2998991168 hasConcept C169760540 @default.
- W2998991168 hasConcept C170493617 @default.
- W2998991168 hasConcept C178790620 @default.
- W2998991168 hasConcept C185592680 @default.
- W2998991168 hasConcept C197341189 @default.
- W2998991168 hasConcept C55493867 @default.
- W2998991168 hasConcept C84416704 @default.
- W2998991168 hasConcept C85528070 @default.
- W2998991168 hasConcept C85789140 @default.
- W2998991168 hasConcept C86803240 @default.
- W2998991168 hasConcept C95444343 @default.
- W2998991168 hasConceptScore W2998991168C116569031 @default.
- W2998991168 hasConceptScore W2998991168C12554922 @default.
- W2998991168 hasConceptScore W2998991168C127445978 @default.
- W2998991168 hasConceptScore W2998991168C16224149 @default.
- W2998991168 hasConceptScore W2998991168C169760540 @default.
- W2998991168 hasConceptScore W2998991168C170493617 @default.
- W2998991168 hasConceptScore W2998991168C178790620 @default.
- W2998991168 hasConceptScore W2998991168C185592680 @default.
- W2998991168 hasConceptScore W2998991168C197341189 @default.
- W2998991168 hasConceptScore W2998991168C55493867 @default.
- W2998991168 hasConceptScore W2998991168C84416704 @default.
- W2998991168 hasConceptScore W2998991168C85528070 @default.
- W2998991168 hasConceptScore W2998991168C85789140 @default.
- W2998991168 hasConceptScore W2998991168C86803240 @default.
- W2998991168 hasConceptScore W2998991168C95444343 @default.
- W2998991168 hasLocation W29989911681 @default.
- W2998991168 hasLocation W29989911682 @default.
- W2998991168 hasLocation W29989911683 @default.