FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2999039827> ?p ?o ?g. }

• W2999039827 endingPage "137" @default.
• W2999039827 startingPage "131" @default.
• W2999039827 abstract "The genome sequence of the hyperthermophilic archaeon Thermococcus kodakarensis contains two putative genes, TK1656 and TK2246, annotated as l-asparaginases. TK1656 has been reported previously. The current report is focused on TK2246, a plant-type l-asparaginase, which consists of 918 nucleotides corresponding to a polypeptide of 306 amino acids. The gene was cloned, expressed in Escherichia coli and the purified gene product was used to determine the properties of the recombinant enzyme. TK2246 was optimally active at 85 °C and pH 7.0 with a specific activity of 767 μmol min-1 mg-1 towards l-asparagine. The enzyme exhibited a 10% activity towards d-asparagine as compared to 100% against l-asparagine. No detectable activity was observed towards l- or d-glutamine. Half-life of the enzyme was nearly 18 h at 85 °C. TK2246 exhibited apparent Km and Vmax values of 3.1 mM and 833 μmol min-1 mg-1, respectively. Activation energy of the reaction, determined from the Arrhenius plot, was 28.3 kJ mol-1. To the best of our knowledge, this is the first characterization of a plant-type l-asparaginase from class Thermococci of phylum Euryarchaeota." @default.
• W2999039827 created "2020-01-23" @default.
• W2999039827 creator A5033613643 @default.
• W2999039827 creator A5063036915 @default.
• W2999039827 creator A5064322239 @default.
• W2999039827 creator A5069478119 @default.
• W2999039827 date "2020-03-01" @default.
• W2999039827 modified "2023-09-25" @default.
• W2999039827 title "Heterologous gene expression and characterization of TK2246, a highly active and thermostable plant type l-asparaginase from Thermococcus kodakarensis" @default.
• W2999039827 cites W1409219335 @default.
• W2999039827 cites W1606775337 @default.
• W2999039827 cites W1964852034 @default.
• W2999039827 cites W1979989054 @default.
• W2999039827 cites W1990481506 @default.
• W2999039827 cites W1995351897 @default.
• W2999039827 cites W2001992796 @default.
• W2999039827 cites W2006291398 @default.
• W2999039827 cites W2024541724 @default.
• W2999039827 cites W2033318279 @default.
• W2999039827 cites W2049393571 @default.
• W2999039827 cites W2050306454 @default.
• W2999039827 cites W2070587052 @default.
• W2999039827 cites W2080051364 @default.
• W2999039827 cites W2080565301 @default.
• W2999039827 cites W2083566665 @default.
• W2999039827 cites W2087870582 @default.
• W2999039827 cites W2096083683 @default.
• W2999039827 cites W2103187103 @default.
• W2999039827 cites W2103347449 @default.
• W2999039827 cites W2150823208 @default.
• W2999039827 cites W2183240495 @default.
• W2999039827 cites W2209354992 @default.
• W2999039827 cites W2313078574 @default.
• W2999039827 cites W2790224627 @default.
• W2999039827 cites W286229235 @default.
• W2999039827 cites W2892579277 @default.
• W2999039827 cites W2898536049 @default.
• W2999039827 doi "https://doi.org/10.1016/j.ijbiomac.2020.01.012" @default.
• W2999039827 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/31923515" @default.
• W2999039827 hasPublicationYear "2020" @default.
• W2999039827 type Work @default.
• W2999039827 sameAs 2999039827 @default.
• W2999039827 citedByCount "16" @default.
• W2999039827 countsByYear W29990398272020 @default.
• W2999039827 countsByYear W29990398272021 @default.
• W2999039827 countsByYear W29990398272022 @default.
• W2999039827 countsByYear W29990398272023 @default.
• W2999039827 crossrefType "journal-article" @default.
• W2999039827 hasAuthorship W2999039827A5033613643 @default.
• W2999039827 hasAuthorship W2999039827A5063036915 @default.
• W2999039827 hasAuthorship W2999039827A5064322239 @default.
• W2999039827 hasAuthorship W2999039827A5069478119 @default.
• W2999039827 hasConcept C104317684 @default.
• W2999039827 hasConcept C108305142 @default.
• W2999039827 hasConcept C153911025 @default.
• W2999039827 hasConcept C181199279 @default.
• W2999039827 hasConcept C185592680 @default.
• W2999039827 hasConcept C2775895851 @default.
• W2999039827 hasConcept C2778461978 @default.
• W2999039827 hasConcept C2778899470 @default.
• W2999039827 hasConcept C2778904437 @default.
• W2999039827 hasConcept C2909962599 @default.
• W2999039827 hasConcept C40767141 @default.
• W2999039827 hasConcept C41183919 @default.
• W2999039827 hasConcept C54355233 @default.
• W2999039827 hasConcept C547475151 @default.
• W2999039827 hasConcept C550995028 @default.
• W2999039827 hasConcept C55493867 @default.
• W2999039827 hasConcept C56856141 @default.
• W2999039827 hasConcept C86803240 @default.
• W2999039827 hasConceptScore W2999039827C104317684 @default.
• W2999039827 hasConceptScore W2999039827C108305142 @default.
• W2999039827 hasConceptScore W2999039827C153911025 @default.
• W2999039827 hasConceptScore W2999039827C181199279 @default.
• W2999039827 hasConceptScore W2999039827C185592680 @default.
• W2999039827 hasConceptScore W2999039827C2775895851 @default.
• W2999039827 hasConceptScore W2999039827C2778461978 @default.
• W2999039827 hasConceptScore W2999039827C2778899470 @default.
• W2999039827 hasConceptScore W2999039827C2778904437 @default.
• W2999039827 hasConceptScore W2999039827C2909962599 @default.
• W2999039827 hasConceptScore W2999039827C40767141 @default.
• W2999039827 hasConceptScore W2999039827C41183919 @default.
• W2999039827 hasConceptScore W2999039827C54355233 @default.
• W2999039827 hasConceptScore W2999039827C547475151 @default.
• W2999039827 hasConceptScore W2999039827C550995028 @default.
• W2999039827 hasConceptScore W2999039827C55493867 @default.
• W2999039827 hasConceptScore W2999039827C56856141 @default.
• W2999039827 hasConceptScore W2999039827C86803240 @default.
• W2999039827 hasLocation W29990398271 @default.
• W2999039827 hasLocation W29990398272 @default.
• W2999039827 hasOpenAccess W2999039827 @default.
• W2999039827 hasPrimaryLocation W29990398271 @default.
• W2999039827 hasRelatedWork W1539688758 @default.
• W2999039827 hasRelatedWork W183374419 @default.
• W2999039827 hasRelatedWork W1838561033 @default.
• W2999039827 hasRelatedWork W2001583806 @default.
• W2999039827 hasRelatedWork W2016628627 @default.
• W2999039827 hasRelatedWork W2025398173 @default.

• next