Matches in SemOpenAlex for { <https://semopenalex.org/work/W2999043712> ?p ?o ?g. }
Showing items 1 to 97 of
97
with 100 items per page.
- W2999043712 endingPage "101897" @default.
- W2999043712 startingPage "101897" @default.
- W2999043712 abstract "The β-adrenergic receptor (β-AR) plays an important role in regulating a variety of cell and organ functions in different animal species and is an important target in asthma pathogenesis and therapy. The β-AR expression and function in equine bronchial epithelial cells (EBEC) were not known but innervation and significant decrease in receptor level were reported in the equine bronchial tissues from asthmatic horses. 125I-iodocyanopindolol (ICYP) binding studies were undertaken in primary freshly isolated and cultured EBEC to identify the presence of the β-ARs. The receptor distribution was assessed using subtype-selective β-AR antagonists (ICI 118 551 (β2) and CGP 20712A (β1). The β-AR function was confirmed by measuring the agonist-induced intracellular cAMP accumulation in freshly isolated and cultured EBEC. In both freshly isolated and cultured EBEC, the specific ICYP binding was saturable and of high affinity. The maximal receptor density (Bmax) was 9763 ± 140 binding sites/cell (mean ± SEM, n = 7) and 10575 ± 194 binding sites/cell (mean ± SEM, n = 5) in freshly isolated and cultured EBEC, respectively. The receptor affinity to the ligand (KD) was also not different between the two cell conditions. ICI 118.551 displaced ICYP with 25 000-fold higher affinity than CGP 20712A. Moreover, in both fresh isolated and cultured EBEC, cAMP-accumulation was stimulated with a rank-order of potency of isoproterenol > adrenaline > noradrenaline. These results highlight the β2-AR to be a key subtype in both freshly isolated and cultured primary EBEC." @default.
- W2999043712 created "2020-01-23" @default.
- W2999043712 creator A5000676734 @default.
- W2999043712 creator A5006552298 @default.
- W2999043712 creator A5019320580 @default.
- W2999043712 creator A5027441350 @default.
- W2999043712 creator A5063881463 @default.
- W2999043712 date "2020-04-01" @default.
- W2999043712 modified "2023-09-27" @default.
- W2999043712 title "Presence and function of β-adrenergic receptors in primary equine bronchial epithelia cells" @default.
- W2999043712 cites W1514600474 @default.
- W2999043712 cites W1538224608 @default.
- W2999043712 cites W1600427338 @default.
- W2999043712 cites W1969732804 @default.
- W2999043712 cites W1983649348 @default.
- W2999043712 cites W1996457811 @default.
- W2999043712 cites W1996972993 @default.
- W2999043712 cites W1997586209 @default.
- W2999043712 cites W2003060940 @default.
- W2999043712 cites W2017795174 @default.
- W2999043712 cites W2023675599 @default.
- W2999043712 cites W2039099150 @default.
- W2999043712 cites W2061711523 @default.
- W2999043712 cites W2062511576 @default.
- W2999043712 cites W2069832564 @default.
- W2999043712 cites W2071696733 @default.
- W2999043712 cites W2074631079 @default.
- W2999043712 cites W2079749197 @default.
- W2999043712 cites W2092790181 @default.
- W2999043712 cites W2108030975 @default.
- W2999043712 cites W2401169647 @default.
- W2999043712 cites W43270296 @default.
- W2999043712 cites W2091961648 @default.
- W2999043712 doi "https://doi.org/10.1016/j.pupt.2020.101897" @default.
- W2999043712 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/31962137" @default.
- W2999043712 hasPublicationYear "2020" @default.
- W2999043712 type Work @default.
- W2999043712 sameAs 2999043712 @default.
- W2999043712 citedByCount "1" @default.
- W2999043712 countsByYear W29990437122021 @default.
- W2999043712 crossrefType "journal-article" @default.
- W2999043712 hasAuthorship W2999043712A5000676734 @default.
- W2999043712 hasAuthorship W2999043712A5006552298 @default.
- W2999043712 hasAuthorship W2999043712A5019320580 @default.
- W2999043712 hasAuthorship W2999043712A5027441350 @default.
- W2999043712 hasAuthorship W2999043712A5063881463 @default.
- W2999043712 hasConcept C126322002 @default.
- W2999043712 hasConcept C134018914 @default.
- W2999043712 hasConcept C153911025 @default.
- W2999043712 hasConcept C170493617 @default.
- W2999043712 hasConcept C2776180500 @default.
- W2999043712 hasConcept C2778938600 @default.
- W2999043712 hasConcept C38533456 @default.
- W2999043712 hasConcept C40465926 @default.
- W2999043712 hasConcept C54355233 @default.
- W2999043712 hasConcept C55493867 @default.
- W2999043712 hasConcept C71924100 @default.
- W2999043712 hasConcept C79879829 @default.
- W2999043712 hasConcept C81885089 @default.
- W2999043712 hasConcept C86803240 @default.
- W2999043712 hasConcept C95444343 @default.
- W2999043712 hasConceptScore W2999043712C126322002 @default.
- W2999043712 hasConceptScore W2999043712C134018914 @default.
- W2999043712 hasConceptScore W2999043712C153911025 @default.
- W2999043712 hasConceptScore W2999043712C170493617 @default.
- W2999043712 hasConceptScore W2999043712C2776180500 @default.
- W2999043712 hasConceptScore W2999043712C2778938600 @default.
- W2999043712 hasConceptScore W2999043712C38533456 @default.
- W2999043712 hasConceptScore W2999043712C40465926 @default.
- W2999043712 hasConceptScore W2999043712C54355233 @default.
- W2999043712 hasConceptScore W2999043712C55493867 @default.
- W2999043712 hasConceptScore W2999043712C71924100 @default.
- W2999043712 hasConceptScore W2999043712C79879829 @default.
- W2999043712 hasConceptScore W2999043712C81885089 @default.
- W2999043712 hasConceptScore W2999043712C86803240 @default.
- W2999043712 hasConceptScore W2999043712C95444343 @default.
- W2999043712 hasFunder F4320323803 @default.
- W2999043712 hasLocation W29990437121 @default.
- W2999043712 hasOpenAccess W2999043712 @default.
- W2999043712 hasPrimaryLocation W29990437121 @default.
- W2999043712 hasRelatedWork W2027913950 @default.
- W2999043712 hasRelatedWork W2033472167 @default.
- W2999043712 hasRelatedWork W2034583712 @default.
- W2999043712 hasRelatedWork W2050423574 @default.
- W2999043712 hasRelatedWork W2052453588 @default.
- W2999043712 hasRelatedWork W2058205365 @default.
- W2999043712 hasRelatedWork W2063619316 @default.
- W2999043712 hasRelatedWork W2070611629 @default.
- W2999043712 hasRelatedWork W2169225653 @default.
- W2999043712 hasRelatedWork W2315166476 @default.
- W2999043712 hasVolume "61" @default.
- W2999043712 isParatext "false" @default.
- W2999043712 isRetracted "false" @default.
- W2999043712 magId "2999043712" @default.
- W2999043712 workType "article" @default.