FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2999051614> ?p ?o ?g. }

• W2999051614 endingPage "5" @default.
• W2999051614 startingPage "1" @default.
• W2999051614 abstract "We present on-chip implementation of an isothermal nucleic acid amplification assay with optomagnetic detection. Isothermal rolling circle amplification (RCA) was designed for the highly specific detection of a single point mutation in the subsequence of the <italic xmlns:mml=http://www.w3.org/1998/Math/MathML xmlns:xlink=http://www.w3.org/1999/xlink>katG</i> catalase peroxidase gene responsible for resistance against the antibiotic isoniazid in <italic xmlns:mml=http://www.w3.org/1998/Math/MathML xmlns:xlink=http://www.w3.org/1999/xlink>Mycobacterium tuberculosis</i> . The assay was integrated using streptavidin-coated magnetic microbeads (MMBs) as a movable substrate to transport the synthetic <italic xmlns:mml=http://www.w3.org/1998/Math/MathML xmlns:xlink=http://www.w3.org/1999/xlink>katG</i> target and products between the sequential processing steps. RCA products were detected via optomagnetic measurements of the binding of functionalized magnetic nanoparticles (MNPs). Binding of MNPs to RCA products leads to an increase of their hydrodynamic size and consequently a shift of the optomagnetic signal to lower frequencies. We first analyzed the affinity of DNA probes used for target capture on MMBs and binding of MNPs to RCA products, respectively. This enabled us to choose conditions where amplification products were released from the MMBs and where their binding to MNPs was optimal. The optimized assay was transferred to a polymer chip with three connected chambers filled with reaction buffers for capture, RCA, and detection. The chip was operated in a setup integrating temperature control, transport of the DNA on magnetic carriers, and dose-dependent detection of MNP clustering. For a total assay time of about 2 h, we demonstrate specific detection of the antibiotic resistance in a synthetic tuberculosis DNA target with a detection limit of 4 pM." @default.
• W2999051614 created "2020-01-23" @default.
• W2999051614 creator A5010084974 @default.
• W2999051614 creator A5014065312 @default.
• W2999051614 creator A5041382343 @default.
• W2999051614 creator A5083425800 @default.
• W2999051614 creator A5087485739 @default.
• W2999051614 date "2020-01-01" @default.
• W2999051614 modified "2023-10-15" @default.
• W2999051614 title "On-Chip DNA Analysis of Tuberculosis Based on Magnetic Nanoparticle Clustering Induced by Rolling Circle Amplification Products" @default.
• W2999051614 cites W2010974451 @default.
• W2999051614 cites W2017647887 @default.
• W2999051614 cites W2027001273 @default.
• W2999051614 cites W2030551376 @default.
• W2999051614 cites W2046854543 @default.
• W2999051614 cites W2066789848 @default.
• W2999051614 cites W2077250713 @default.
• W2999051614 cites W2094961994 @default.
• W2999051614 cites W2206007518 @default.
• W2999051614 cites W2220709107 @default.
• W2999051614 cites W2300703173 @default.
• W2999051614 cites W2590790205 @default.
• W2999051614 cites W2746232001 @default.
• W2999051614 cites W2770461981 @default.
• W2999051614 cites W2787305095 @default.
• W2999051614 cites W2807180755 @default.
• W2999051614 cites W2891283853 @default.
• W2999051614 cites W2955505923 @default.
• W2999051614 cites W2959993388 @default.
• W2999051614 doi "https://doi.org/10.1109/lmag.2019.2959545" @default.
• W2999051614 hasPublicationYear "2020" @default.
• W2999051614 type Work @default.
• W2999051614 sameAs 2999051614 @default.
• W2999051614 citedByCount "6" @default.
• W2999051614 countsByYear W29990516142021 @default.
• W2999051614 countsByYear W29990516142022 @default.
• W2999051614 countsByYear W29990516142023 @default.
• W2999051614 crossrefType "journal-article" @default.
• W2999051614 hasAuthorship W2999051614A5010084974 @default.
• W2999051614 hasAuthorship W2999051614A5014065312 @default.
• W2999051614 hasAuthorship W2999051614A5041382343 @default.
• W2999051614 hasAuthorship W2999051614A5083425800 @default.
• W2999051614 hasAuthorship W2999051614A5087485739 @default.
• W2999051614 hasBestOaLocation W29990516142 @default.
• W2999051614 hasConcept C142724271 @default.
• W2999051614 hasConcept C153911025 @default.
• W2999051614 hasConcept C155672457 @default.
• W2999051614 hasConcept C171250308 @default.
• W2999051614 hasConcept C185592680 @default.
• W2999051614 hasConcept C192562407 @default.
• W2999051614 hasConcept C21951064 @default.
• W2999051614 hasConcept C2756471 @default.
• W2999051614 hasConcept C2777975735 @default.
• W2999051614 hasConcept C2781069245 @default.
• W2999051614 hasConcept C552990157 @default.
• W2999051614 hasConcept C55493867 @default.
• W2999051614 hasConcept C60635243 @default.
• W2999051614 hasConcept C71924100 @default.
• W2999051614 hasConcept C7452308 @default.
• W2999051614 hasConcept C86803240 @default.
• W2999051614 hasConcept C87023908 @default.
• W2999051614 hasConceptScore W2999051614C142724271 @default.
• W2999051614 hasConceptScore W2999051614C153911025 @default.
• W2999051614 hasConceptScore W2999051614C155672457 @default.
• W2999051614 hasConceptScore W2999051614C171250308 @default.
• W2999051614 hasConceptScore W2999051614C185592680 @default.
• W2999051614 hasConceptScore W2999051614C192562407 @default.
• W2999051614 hasConceptScore W2999051614C21951064 @default.
• W2999051614 hasConceptScore W2999051614C2756471 @default.
• W2999051614 hasConceptScore W2999051614C2777975735 @default.
• W2999051614 hasConceptScore W2999051614C2781069245 @default.
• W2999051614 hasConceptScore W2999051614C552990157 @default.
• W2999051614 hasConceptScore W2999051614C55493867 @default.
• W2999051614 hasConceptScore W2999051614C60635243 @default.
• W2999051614 hasConceptScore W2999051614C71924100 @default.
• W2999051614 hasConceptScore W2999051614C7452308 @default.
• W2999051614 hasConceptScore W2999051614C86803240 @default.
• W2999051614 hasConceptScore W2999051614C87023908 @default.
• W2999051614 hasFunder F4320322928 @default.
• W2999051614 hasLocation W29990516141 @default.
• W2999051614 hasLocation W29990516142 @default.
• W2999051614 hasLocation W29990516143 @default.
• W2999051614 hasOpenAccess W2999051614 @default.
• W2999051614 hasPrimaryLocation W29990516141 @default.
• W2999051614 hasRelatedWork W1975061774 @default.
• W2999051614 hasRelatedWork W2003087030 @default.
• W2999051614 hasRelatedWork W2087844283 @default.
• W2999051614 hasRelatedWork W2349599514 @default.
• W2999051614 hasRelatedWork W2378351399 @default.
• W2999051614 hasRelatedWork W2385043201 @default.
• W2999051614 hasRelatedWork W2390156094 @default.
• W2999051614 hasRelatedWork W2945075252 @default.
• W2999051614 hasRelatedWork W3187545916 @default.
• W2999051614 hasRelatedWork W3203345307 @default.
• W2999051614 hasVolume "11" @default.
• W2999051614 isParatext "false" @default.
• W2999051614 isRetracted "false" @default.
• W2999051614 magId "2999051614" @default.

• next