FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2999224854> ?p ?o ?g. }

• W2999224854 abstract "The increasing number of immunocompromised people has made invasive fungal infections more common. The antifungal armamentarium, in contrast, is limited to a few classes of drugs, with frequent toxicity and low efficacy pointing to the need for new agents. Antibodies are great candidates for novel antifungals, as their specificity can result in lower toxicity. Additionally, the immunomodulatory activity of antibodies could treat the underlying cause of many invasive mycoses, immune disfunction. In a previous comparative genomics study, we identified several potential targets for novel antifungals. Here we validate one of these targets, thioredoxin reductase (TRR1), to produce antibodies that could be useful therapeutic tools. Recombinant TRR1 proteins were produced by heterologous expression in Escherichia coli of genes encoding the proteins from Candida albicans, Cryptococcus neoformans, and Paracoccidioides lutzii. These proteins were then used to immunize mice, followed by detection of serum antibodies against them by ELISA and western blot. A first set of experiments in which individual mice were immunized repeatedly with TRR1 from a single species showed that all three were highly immunogenic, inducing mostly IgG1 antibodies, and that antibodies produced against one species cross-reacted with the others. In a second experiment, individual mice were immunized three times, each with the protein from a different species. The high titers of antibodies confirmed the presence of antigenic epitopes that were conserved in fungi but absent in humans. Immunofluorescence with sera from these immunized mice detected the protein in the cytoplasm and on the cell surface of fungi from all three species. These results validate TRR1 as a good target for potentially broad-spectrum antifungal antibodies." @default.
• W2999224854 created "2020-01-23" @default.
• W2999224854 creator A5009963511 @default.
• W2999224854 creator A5022852269 @default.
• W2999224854 creator A5048739701 @default.
• W2999224854 creator A5054239796 @default.
• W2999224854 creator A5056557475 @default.
• W2999224854 creator A5061287481 @default.
• W2999224854 creator A5077902719 @default.
• W2999224854 creator A5089556605 @default.
• W2999224854 date "2020-01-09" @default.
• W2999224854 modified "2023-10-18" @default.
• W2999224854 title "Thioredoxin Reductase 1 Is a Highly Immunogenic Cell Surface Antigen in Paracoccidioides spp., Candida albicans, and Cryptococcus neoformans" @default.
• W2999224854 cites W1928258841 @default.
• W2999224854 cites W1965606695 @default.
• W2999224854 cites W1998437697 @default.
• W2999224854 cites W2004087055 @default.
• W2999224854 cites W2046697881 @default.
• W2999224854 cites W2049036392 @default.
• W2999224854 cites W2053512152 @default.
• W2999224854 cites W2055850006 @default.
• W2999224854 cites W2069395166 @default.
• W2999224854 cites W2110298304 @default.
• W2999224854 cites W2114886688 @default.
• W2999224854 cites W2118686778 @default.
• W2999224854 cites W2119235066 @default.
• W2999224854 cites W2125636530 @default.
• W2999224854 cites W2126076750 @default.
• W2999224854 cites W2134941291 @default.
• W2999224854 cites W2149149952 @default.
• W2999224854 cites W2151417485 @default.
• W2999224854 cites W2169656611 @default.
• W2999224854 cites W2171146043 @default.
• W2999224854 cites W2173186713 @default.
• W2999224854 cites W2187008277 @default.
• W2999224854 cites W2258912774 @default.
• W2999224854 cites W2261930170 @default.
• W2999224854 cites W2273817799 @default.
• W2999224854 cites W2297075820 @default.
• W2999224854 cites W2337229254 @default.
• W2999224854 cites W2409377496 @default.
• W2999224854 cites W2508618039 @default.
• W2999224854 cites W2553189343 @default.
• W2999224854 cites W2575466306 @default.
• W2999224854 cites W2594453394 @default.
• W2999224854 cites W2753844820 @default.
• W2999224854 cites W2767031614 @default.
• W2999224854 cites W2767349847 @default.
• W2999224854 cites W2775529910 @default.
• W2999224854 cites W2783102765 @default.
• W2999224854 cites W2790546589 @default.
• W2999224854 cites W2896191321 @default.
• W2999224854 cites W2921155926 @default.
• W2999224854 cites W2938574745 @default.
• W2999224854 cites W70375962 @default.
• W2999224854 doi "https://doi.org/10.3389/fmicb.2019.02930" @default.
• W2999224854 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/6964600" @default.
• W2999224854 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/31993026" @default.
• W2999224854 hasPublicationYear "2020" @default.
• W2999224854 type Work @default.
• W2999224854 sameAs 2999224854 @default.
• W2999224854 citedByCount "2" @default.
• W2999224854 countsByYear W29992248542021 @default.
• W2999224854 countsByYear W29992248542023 @default.
• W2999224854 crossrefType "journal-article" @default.
• W2999224854 hasAuthorship W2999224854A5009963511 @default.
• W2999224854 hasAuthorship W2999224854A5022852269 @default.
• W2999224854 hasAuthorship W2999224854A5048739701 @default.
• W2999224854 hasAuthorship W2999224854A5054239796 @default.
• W2999224854 hasAuthorship W2999224854A5056557475 @default.
• W2999224854 hasAuthorship W2999224854A5061287481 @default.
• W2999224854 hasAuthorship W2999224854A5077902719 @default.
• W2999224854 hasAuthorship W2999224854A5089556605 @default.
• W2999224854 hasBestOaLocation W29992248541 @default.
• W2999224854 hasConcept C145081991 @default.
• W2999224854 hasConcept C147483822 @default.
• W2999224854 hasConcept C159654299 @default.
• W2999224854 hasConcept C195616568 @default.
• W2999224854 hasConcept C203014093 @default.
• W2999224854 hasConcept C2777681368 @default.
• W2999224854 hasConcept C2777893358 @default.
• W2999224854 hasConcept C2780917455 @default.
• W2999224854 hasConcept C86803240 @default.
• W2999224854 hasConcept C89423630 @default.
• W2999224854 hasConceptScore W2999224854C145081991 @default.
• W2999224854 hasConceptScore W2999224854C147483822 @default.
• W2999224854 hasConceptScore W2999224854C159654299 @default.
• W2999224854 hasConceptScore W2999224854C195616568 @default.
• W2999224854 hasConceptScore W2999224854C203014093 @default.
• W2999224854 hasConceptScore W2999224854C2777681368 @default.
• W2999224854 hasConceptScore W2999224854C2777893358 @default.
• W2999224854 hasConceptScore W2999224854C2780917455 @default.
• W2999224854 hasConceptScore W2999224854C86803240 @default.
• W2999224854 hasConceptScore W2999224854C89423630 @default.
• W2999224854 hasLocation W29992248541 @default.
• W2999224854 hasLocation W29992248542 @default.
• W2999224854 hasLocation W29992248543 @default.
• W2999224854 hasLocation W29992248544 @default.
• W2999224854 hasOpenAccess W2999224854 @default.
• W2999224854 hasPrimaryLocation W29992248541 @default.

• next