Matches in SemOpenAlex for { <https://semopenalex.org/work/W2999342835> ?p ?o ?g. }
Showing items 1 to 81 of
81
with 100 items per page.
- W2999342835 abstract "Abstract Personalized medicine focused at individual patient's treatment by matching between the genetic information hidden at the DNA and medication. Single nucleotide mutations (SNPs) play a major role in genetic variability and the ability to react to medications.BRAF gene is involved in solid tumors like malignant melanoma and thyroid. BRAF is a serine/threonine protein kinase, activates the MAPK/ERK signaling pathway.The development of specific BRAF inhibitors that work only on the SNP V600E1 (T > A at position 1799) such as vemurafenib (Zelboraf) gave push to personalized medicine as 50% of metastatic melanoma patients carry this mutation. Those who carry the mutation demonstrate better response to vemurafenib than others. By comparison between 2 methods to detect SNPs in the BRAF gene, we show here that INFINITI™ Analyzer is the most accurate and reliable method. Methods BRAF mutation analysis by Length Analysis. We used allele-specific DNA with two specific forward primers for the mutant and the wild-type alleles and one common reverse primer. Mutated allele gives fragment of 160 bp and the wild type allele gives fragment of 157 bp. PCR was carried out and products were separated on sequencer and analyzed using peak scanner. BRAF mutation detected by AutoGenomics INFINITI-BRAF Assay. Following PCR amplification a removal of the non-incorporated dNTPs was performed.The products were loaded on BioFilmChip microarrays together with analyte specific primer extension fluorescently label. Results and Discussion We tested 101 samples and compare the results from the AutoGenomics INFINITI Assay with those of the DNA Fragment Analysis. The result reveals that the INFINITI BRAF Assay is sensitive and most easy to work with. That assay is almost fully automated and need shorter time for processing and lower concentration of DNA. The last issue is more important in cases of thyroid cancer were samples are taken by fine needle aspiration. We achieved 97% of concordance between both methods. All 3 discrepant cases were of V600E1 mutation detection that were found only by the INFINITI-BRAF. The ability of the INFINITI to detect mutation is 1 mutant cell I population of 100 cells. Both methods are suitable for SNP detection." @default.
- W2999342835 created "2020-01-23" @default.
- W2999342835 creator A5007665681 @default.
- W2999342835 creator A5032925798 @default.
- W2999342835 creator A5062199973 @default.
- W2999342835 creator A5072041544 @default.
- W2999342835 date "2012-06-01" @default.
- W2999342835 modified "2023-09-26" @default.
- W2999342835 title "P1.03 SNPS Detection in the Braf Gene" @default.
- W2999342835 doi "https://doi.org/10.1016/s0923-7534(20)31294-1" @default.
- W2999342835 hasPublicationYear "2012" @default.
- W2999342835 type Work @default.
- W2999342835 sameAs 2999342835 @default.
- W2999342835 citedByCount "0" @default.
- W2999342835 crossrefType "journal-article" @default.
- W2999342835 hasAuthorship W2999342835A5007665681 @default.
- W2999342835 hasAuthorship W2999342835A5032925798 @default.
- W2999342835 hasAuthorship W2999342835A5062199973 @default.
- W2999342835 hasAuthorship W2999342835A5072041544 @default.
- W2999342835 hasBestOaLocation W29993428351 @default.
- W2999342835 hasConcept C104317684 @default.
- W2999342835 hasConcept C135763542 @default.
- W2999342835 hasConcept C153209595 @default.
- W2999342835 hasConcept C153911025 @default.
- W2999342835 hasConcept C17757408 @default.
- W2999342835 hasConcept C178790620 @default.
- W2999342835 hasConcept C180754005 @default.
- W2999342835 hasConcept C185592680 @default.
- W2999342835 hasConcept C2776131300 @default.
- W2999342835 hasConcept C2777563447 @default.
- W2999342835 hasConcept C2777658100 @default.
- W2999342835 hasConcept C2994587330 @default.
- W2999342835 hasConcept C501734568 @default.
- W2999342835 hasConcept C502942594 @default.
- W2999342835 hasConcept C54355233 @default.
- W2999342835 hasConcept C71924100 @default.
- W2999342835 hasConcept C86803240 @default.
- W2999342835 hasConceptScore W2999342835C104317684 @default.
- W2999342835 hasConceptScore W2999342835C135763542 @default.
- W2999342835 hasConceptScore W2999342835C153209595 @default.
- W2999342835 hasConceptScore W2999342835C153911025 @default.
- W2999342835 hasConceptScore W2999342835C17757408 @default.
- W2999342835 hasConceptScore W2999342835C178790620 @default.
- W2999342835 hasConceptScore W2999342835C180754005 @default.
- W2999342835 hasConceptScore W2999342835C185592680 @default.
- W2999342835 hasConceptScore W2999342835C2776131300 @default.
- W2999342835 hasConceptScore W2999342835C2777563447 @default.
- W2999342835 hasConceptScore W2999342835C2777658100 @default.
- W2999342835 hasConceptScore W2999342835C2994587330 @default.
- W2999342835 hasConceptScore W2999342835C501734568 @default.
- W2999342835 hasConceptScore W2999342835C502942594 @default.
- W2999342835 hasConceptScore W2999342835C54355233 @default.
- W2999342835 hasConceptScore W2999342835C71924100 @default.
- W2999342835 hasConceptScore W2999342835C86803240 @default.
- W2999342835 hasLocation W29993428351 @default.
- W2999342835 hasOpenAccess W2999342835 @default.
- W2999342835 hasPrimaryLocation W29993428351 @default.
- W2999342835 hasRelatedWork W1523968588 @default.
- W2999342835 hasRelatedWork W1985697914 @default.
- W2999342835 hasRelatedWork W2006412460 @default.
- W2999342835 hasRelatedWork W2041082881 @default.
- W2999342835 hasRelatedWork W2046871652 @default.
- W2999342835 hasRelatedWork W2051050264 @default.
- W2999342835 hasRelatedWork W2057800132 @default.
- W2999342835 hasRelatedWork W2223150849 @default.
- W2999342835 hasRelatedWork W2806074576 @default.
- W2999342835 hasRelatedWork W2809347889 @default.
- W2999342835 hasRelatedWork W2999183162 @default.
- W2999342835 hasRelatedWork W3030153370 @default.
- W2999342835 hasRelatedWork W3194719825 @default.
- W2999342835 hasRelatedWork W940172838 @default.
- W2999342835 hasRelatedWork W2245272616 @default.
- W2999342835 hasRelatedWork W2461620165 @default.
- W2999342835 hasRelatedWork W2819542344 @default.
- W2999342835 hasRelatedWork W2925991305 @default.
- W2999342835 hasRelatedWork W3073765059 @default.
- W2999342835 hasRelatedWork W3178923709 @default.
- W2999342835 isParatext "false" @default.
- W2999342835 isRetracted "false" @default.
- W2999342835 magId "2999342835" @default.
- W2999342835 workType "article" @default.