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• W2999462721 abstract "Abstract STUDY QUESTION What is the effect of antioxidants acetyl-L-carnitine, N-acetyl-L-cysteine and α-lipoic acid (A3) in vitrification and warming solutions on mouse blastocyst development and viability? SUMMARY ANSWER The combination of three antioxidants in vitrification solutions resulted in mouse blastocysts with higher developmental potential in vitro and increased viability as assessed by both an outgrowth model in vitro and fetal development following uterine transfer. WHAT IS KNOWN ALREADY The antioxidant combination of acetyl-L-carnitine, N-acetyl-L-cysteine and α-lipoic acid present in IVF handling and embryo culture media has significant beneficial effects on mouse embryo and fetal development, especially under oxidative stress. STUDY DESIGN, SIZE, DURATION The study was a laboratory-based analysis of an animal model. Rapid cooling through vitrification was conducted on F1 mouse blastocysts, with antioxidants (A3) supplemented in vitrification and/or warming solutions, followed by culture and embryo transfer. PARTICIPANTS/MATERIALS, SETTINGS, METHODS Pronucleate oocytes were collected and cultured in groups to Day 4 blastocysts. Expanded blastocysts were vitrified and warmed in solutions with and without the A3 antioxidants and cultured for a further 24 h. Blastocyst cell number and allocation, apoptosis and histone acetylation levels were all quantified, and viability through outgrowths and transfers assessed. MAIN RESULTS AND THE ROLE OF CHANCE Mouse blastocysts vitrified with no antioxidants had significantly lower cell numbers (P &lt; 0.001) and higher apoptotic cells (P &lt; 0.05) compared to non-vitrified embryos. Addition of combined A3 antioxidants to the vitrification and warming solutions resulted in a significant increase in inner cell mass cell (ICM) number (P &lt; 0.001) and total cell number (P &lt; 0.01), and an increase in outgrowth area (P &lt; 0.05), which correlated with the increased fetal weight (P &lt; 0.05), crown rump length (P &lt; 0.05) and limb development (P &lt; 0.05) determined following transfer compared to embryos with no antioxidants. Furthermore, while blastocyst vitrification significantly reduced acetylation levels (P &lt; 0.05) compared to non-vitrified embryos, the inclusion of A3 antioxidants helped to ameliorate this. LIMITATIONS, REASONS FOR CAUTION Embryo development was only examined in the mouse. WIDER IMPLICATIONS OF THE FINDINGS Results in this study demonstrate that vitrification and warming of blastocysts have significant detrimental effects on embryo histone acetylation and subsequent viability. The presence of antioxidants in the vitrification solutions helps to alleviate the negative effects of cryopreservation. Our data indicate that antioxidants need to be present in the medium at the time of exposure to increased oxidative stress associated with vitrification and that prior exposure (i.e. during culture or IVF alone) is insufficient to protect cells against cryo-induced injury. Hence, A3 antioxidants may assist in maintaining the viability of vitrified human embryos in ART through the reduction of oxidative stress. STUDY FUNDING/COMPETING INTEREST(S) This work was funded by a research grant from Vitrolife AB (Sweden). The authors have no conflict of interest to declare." @default.
• W2999462721 created "2020-01-23" @default.
• W2999462721 creator A5023601676 @default.
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• W2999462721 date "2020-01-01" @default.
• W2999462721 modified "2023-10-16" @default.
• W2999462721 title "Antioxidants increase blastocyst cryosurvival and viability post-vitrification" @default.
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• W2999462721 cites W1599464745 @default.
• W2999462721 cites W1677653338 @default.
• W2999462721 cites W1746091885 @default.
• W2999462721 cites W1934389460 @default.
• W2999462721 cites W1966496209 @default.
• W2999462721 cites W1968553683 @default.
• W2999462721 cites W1971448178 @default.
• W2999462721 cites W1974978813 @default.
• W2999462721 cites W1980813479 @default.
• W2999462721 cites W1985193199 @default.
• W2999462721 cites W1996395636 @default.
• W2999462721 cites W1997964174 @default.
• W2999462721 cites W1998243633 @default.
• W2999462721 cites W2004563349 @default.
• W2999462721 cites W2009455793 @default.
• W2999462721 cites W2009609926 @default.
• W2999462721 cites W2019086157 @default.
• W2999462721 cites W2020944325 @default.
• W2999462721 cites W2021395614 @default.
• W2999462721 cites W2027181956 @default.
• W2999462721 cites W2031323095 @default.
• W2999462721 cites W2031626816 @default.
• W2999462721 cites W2036422494 @default.
• W2999462721 cites W2038052356 @default.
• W2999462721 cites W2038117472 @default.
• W2999462721 cites W2041088640 @default.
• W2999462721 cites W2048479598 @default.
• W2999462721 cites W2051699328 @default.
• W2999462721 cites W2052205195 @default.
• W2999462721 cites W2065494919 @default.
• W2999462721 cites W2073843783 @default.
• W2999462721 cites W2076692610 @default.
• W2999462721 cites W2076977009 @default.
• W2999462721 cites W2077952338 @default.
• W2999462721 cites W2083681165 @default.
• W2999462721 cites W2084529284 @default.
• W2999462721 cites W2085982661 @default.
• W2999462721 cites W2088935692 @default.
• W2999462721 cites W2095574453 @default.
• W2999462721 cites W2108068540 @default.
• W2999462721 cites W2109963570 @default.
• W2999462721 cites W2124128749 @default.
• W2999462721 cites W2124740130 @default.
• W2999462721 cites W2125114137 @default.
• W2999462721 cites W2125822731 @default.
• W2999462721 cites W2126460503 @default.
• W2999462721 cites W2132795811 @default.
• W2999462721 cites W2136268403 @default.
• W2999462721 cites W2144062165 @default.
• W2999462721 cites W2145780187 @default.
• W2999462721 cites W2147038326 @default.
• W2999462721 cites W2151583121 @default.
• W2999462721 cites W2151721239 @default.
• W2999462721 cites W2152696378 @default.
• W2999462721 cites W2156861769 @default.
• W2999462721 cites W2164645631 @default.
• W2999462721 cites W2186307437 @default.
• W2999462721 cites W2292991999 @default.
• W2999462721 cites W2350251354 @default.
• W2999462721 cites W2404986598 @default.
• W2999462721 cites W2410663019 @default.
• W2999462721 cites W2419604885 @default.
• W2999462721 cites W2499215178 @default.
• W2999462721 cites W2513338903 @default.
• W2999462721 cites W2554189630 @default.
• W2999462721 cites W2613623665 @default.
• W2999462721 cites W2767467045 @default.
• W2999462721 cites W2772242391 @default.
• W2999462721 cites W2799600507 @default.
• W2999462721 cites W2909079157 @default.
• W2999462721 cites W2916530067 @default.
• W2999462721 cites W2918514499 @default.
• W2999462721 cites W2923104520 @default.
• W2999462721 cites W2952044912 @default.
• W2999462721 cites W2972916572 @default.
• W2999462721 doi "https://doi.org/10.1093/humrep/dez243" @default.
• W2999462721 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/31916568" @default.
• W2999462721 hasPublicationYear "2020" @default.
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