Matches in SemOpenAlex for { <https://semopenalex.org/work/W2999695513> ?p ?o ?g. }
Showing items 1 to 83 of
83
with 100 items per page.
- W2999695513 abstract "Abstract Introduction Malignant cells require high energy via glycolytic generation of ATP for cell proliferation and survival, a process that is thought to be mediated by glucose transporters (GLUTs). Notably, GLUT1 is overexpressed and confers poor prognosis in a wide range of solid tumors, including liver tumors. A recent study has shown that expression of GLUT1 is increased in Hepatocellular Carcinoma (HCC) and promotes tumorigenesis. Conversely, suppression of GLUT1 expression by small interfering RNA significantly impaired tumorigenicity of HCC cells in in vitro models, suggesting that GLUT1 as an innovative therapeutic target for this highly aggressive tumor. Positron Emission Tomography (PET) using the radiolabeled glucose analogue 18F-FDG enables the detection of increased glycolysis events in cancer cells. Glucose is transported into the cell mainly through the GLUT-1 and -3. Gossypol is a natural compound that has demonstrated anticancer effect in several tumor types. It is known that gossypol is a competitive inhibitor of the GLUT1. The aim of this study is to test the anticancer effect of gossypol in HCC cell lines, as well as check its effect on 18F-FDG uptake. Methods The cell lines used are HepG2 (wp53), HuH7 (mp53) and Hep3b2.1-7 (p53 null). These cell lines were propagated in the presence of gossypol in several concentrations. Cell proliferation was evaluated by the MTT test, in order to obtain dose-response curves. After that the type of cell death (necrosis or apoptosis) and the percentage of live cells were assessed by flow cytometry for the concentration of drug able to induce half maximal inhibitory concentration (IC50).For uptake studies, 18F-FDG was incubated in a cell suspension with 2×106 cells/ml (25µCi/ml) in cells pre-incubated with gossypol and control cells. Samples were collected to Eppendorf tubes for tracer uptake calculation. Eppendorfs were then centrifuged and radioactivity of cell pellets and supernatants was measured with a well-type gamma counter. Results This study demonstrates that the concentrations of gossypol necessary to achieve the IC50 is higher for HuH7 cell line (IC50(24h)=7.5µM). Interestingly, the cell line more sensitive to this compound is Hep3B2.1-7 (IC50(24h)=2.6µM), which may be related to the fact that these cells do not express p53. The cellular response is dependent on time, for each of the cell lines studied. Flow cytometry results show that gossypol induces high apoptosis in HepG2 and HuH7 cell lines with lower induction of necrosis. In Hep3B2.1-7 cell line there is a balance between apoptosis and necrosis. In addition, for the three cell lines studied, gossypol was able to decrease the percentage of 18F-FDG uptake in the similar way. Conclusion These results shown that gossypol has anti-proliferative effect on HCC. Gossypol also has shown ability to decrease the uptake of 18F-FDG in the three cell lines. More studies must be done to clarify the role of p53 in the anti-proliferative effect of this compound in HCC as well as the effect in the uptake of the 18F-FDG. These preliminary results are quite useful in clinical practice." @default.
- W2999695513 created "2020-01-23" @default.
- W2999695513 creator A5042166089 @default.
- W2999695513 creator A5046477501 @default.
- W2999695513 creator A5063571512 @default.
- W2999695513 creator A5089221056 @default.
- W2999695513 date "2012-06-01" @default.
- W2999695513 modified "2023-10-15" @default.
- W2999695513 title "P-0076 18F-FDG and Gossypol: Studies in Hepatocellular Carcinoma" @default.
- W2999695513 doi "https://doi.org/10.1016/s0923-7534(20)30283-0" @default.
- W2999695513 hasPublicationYear "2012" @default.
- W2999695513 type Work @default.
- W2999695513 sameAs 2999695513 @default.
- W2999695513 citedByCount "0" @default.
- W2999695513 crossrefType "journal-article" @default.
- W2999695513 hasAuthorship W2999695513A5042166089 @default.
- W2999695513 hasAuthorship W2999695513A5046477501 @default.
- W2999695513 hasAuthorship W2999695513A5063571512 @default.
- W2999695513 hasAuthorship W2999695513A5089221056 @default.
- W2999695513 hasBestOaLocation W29996955131 @default.
- W2999695513 hasConcept C121608353 @default.
- W2999695513 hasConcept C126322002 @default.
- W2999695513 hasConcept C1491633281 @default.
- W2999695513 hasConcept C161573976 @default.
- W2999695513 hasConcept C190283241 @default.
- W2999695513 hasConcept C203014093 @default.
- W2999695513 hasConcept C2776653637 @default.
- W2999695513 hasConcept C2777952866 @default.
- W2999695513 hasConcept C2779306644 @default.
- W2999695513 hasConcept C502942594 @default.
- W2999695513 hasConcept C54355233 @default.
- W2999695513 hasConcept C553184892 @default.
- W2999695513 hasConcept C55493867 @default.
- W2999695513 hasConcept C555283112 @default.
- W2999695513 hasConcept C62112901 @default.
- W2999695513 hasConcept C71924100 @default.
- W2999695513 hasConcept C81885089 @default.
- W2999695513 hasConcept C86803240 @default.
- W2999695513 hasConceptScore W2999695513C121608353 @default.
- W2999695513 hasConceptScore W2999695513C126322002 @default.
- W2999695513 hasConceptScore W2999695513C1491633281 @default.
- W2999695513 hasConceptScore W2999695513C161573976 @default.
- W2999695513 hasConceptScore W2999695513C190283241 @default.
- W2999695513 hasConceptScore W2999695513C203014093 @default.
- W2999695513 hasConceptScore W2999695513C2776653637 @default.
- W2999695513 hasConceptScore W2999695513C2777952866 @default.
- W2999695513 hasConceptScore W2999695513C2779306644 @default.
- W2999695513 hasConceptScore W2999695513C502942594 @default.
- W2999695513 hasConceptScore W2999695513C54355233 @default.
- W2999695513 hasConceptScore W2999695513C553184892 @default.
- W2999695513 hasConceptScore W2999695513C55493867 @default.
- W2999695513 hasConceptScore W2999695513C555283112 @default.
- W2999695513 hasConceptScore W2999695513C62112901 @default.
- W2999695513 hasConceptScore W2999695513C71924100 @default.
- W2999695513 hasConceptScore W2999695513C81885089 @default.
- W2999695513 hasConceptScore W2999695513C86803240 @default.
- W2999695513 hasLocation W29996955131 @default.
- W2999695513 hasOpenAccess W2999695513 @default.
- W2999695513 hasPrimaryLocation W29996955131 @default.
- W2999695513 hasRelatedWork W2028335552 @default.
- W2999695513 hasRelatedWork W2055782963 @default.
- W2999695513 hasRelatedWork W2087105518 @default.
- W2999695513 hasRelatedWork W2160192777 @default.
- W2999695513 hasRelatedWork W2350184831 @default.
- W2999695513 hasRelatedWork W2351789248 @default.
- W2999695513 hasRelatedWork W2352022712 @default.
- W2999695513 hasRelatedWork W2367404824 @default.
- W2999695513 hasRelatedWork W2372999700 @default.
- W2999695513 hasRelatedWork W2373674561 @default.
- W2999695513 hasRelatedWork W2375776377 @default.
- W2999695513 hasRelatedWork W2378536668 @default.
- W2999695513 hasRelatedWork W2386670487 @default.
- W2999695513 hasRelatedWork W2389080897 @default.
- W2999695513 hasRelatedWork W2410955998 @default.
- W2999695513 hasRelatedWork W2419125918 @default.
- W2999695513 hasRelatedWork W2897966308 @default.
- W2999695513 hasRelatedWork W2999332592 @default.
- W2999695513 hasRelatedWork W3031964414 @default.
- W2999695513 hasRelatedWork W3142287132 @default.
- W2999695513 isParatext "false" @default.
- W2999695513 isRetracted "false" @default.
- W2999695513 magId "2999695513" @default.
- W2999695513 workType "article" @default.