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- W2999765220 abstract "Campylobacter species, particularly thermotolerant Campylobacter spp., such as C. jejuni, are major human foodborne pathogens worldwide. Culture methods are routinely used for the detection of this organism. Campylobacter-like colonies can be difficult to identify on agar plates overgrown with competitive bacteria. An alternative, simple and rapid confirmation testing without further tedious biochemical tests would be useful. Previously we developed a colony blot immunoassay (CBI) using a specific monoclonal antibody (Mab M1169) for rapid screening, confirmation and quantification of campylobacters on agar plates. This assay showed 100% correlation with the culture method from 21 food samples experimentally inoculated with the most common thermotolerant campylobacters, and over 100 natural non-intentionally inoculated samples of chicken meat, chicken faeces and vegetables. The Mab 1169 was able to specifically detect the most common thermotolerant Campylobacter spp. Further studies have been conducted to improve the assay procedures with semi-automation and to understand the nature of antigen(s) reacting with this antibody using mass spectrometry analysis. It seems that the antibody recognizes an epitope(s) shared between several proteins including Chaperone GroEL and Flagellin A or an antigen bound to these proteins. This chapter provides an updated relevant literature review, detailed information on the development and improvement of the CBI and the studies on the antigen(s) reacting with the M1169. Overall, the CBI assay described here can be used in quantitative study for immediate or archival usage, and for diagnostic test to preliminarily confirm the presence of thermotolerant Campylobacter on agar plates." @default.
- W2999765220 created "2020-01-23" @default.
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- W2999765220 date "2020-01-01" @default.
- W2999765220 modified "2023-09-25" @default.
- W2999765220 title "Development and improvement of a colony blot immunoassay for the detection of thermotolerant Campylobacter species" @default.
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- W2999765220 doi "https://doi.org/10.1016/bs.mim.2019.11.005" @default.
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