FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2999851567> ?p ?o ?g. }

• W2999851567 endingPage "104013" @default.
• W2999851567 startingPage "104013" @default.
• W2999851567 abstract "Leptinotarsa decemlineata (Say), commonly known as the Colorado potato beetle (CPB), is an agricultural important pest for potatoes and other solanaceous plants. The CRISPR/Cas system is an efficient genome editing technology, which could be exploited to study the biology of CPB and possibly also lead to the development of better environmentally friendly pest management strategies. However, the use of CRISPR/Cas9 has been limited to only a few model insects. Here, for the first time, a CRISPR/Cas9 protocol for mutagenesis studies in CPB was developed. A gene with a clear phenotype such as the vestigial gene (vest), known to be involved in wing development in other insect species, was selected as a good indicator for the knockout study. First, vest was functionally characterized in CPB by using RNAi technology for knockdown studies. Once the expected deformed wing phenotypes were observed, a CRISPR/Cas9 work flow was established for mutagenesis in CPB. By co-injecting the Cas9 protein and a vest-guide RNA into 539 CPB eggs of <1 h old, sixty-two successfully developed to adults, among which mutation in the vest loci was confirmed in 5 of the 18 wingless CPBs (29% phenotypic mutation efficiency). The mutation in vest resulted in a clear phenotype in the CPBs, which developed to adulthood with no hindwing and elytron formed. Altogether, this study provides for the first time a useful methodology involving the use of the CRISPR/Cas9 system for mutagenesis studies in one of the most important pest insects." @default.
• W2999851567 created "2020-01-23" @default.
• W2999851567 creator A5034229389 @default.
• W2999851567 creator A5065087222 @default.
• W2999851567 creator A5066515722 @default.
• W2999851567 creator A5089906704 @default.
• W2999851567 date "2020-02-01" @default.
• W2999851567 modified "2023-10-18" @default.
• W2999851567 title "First report on CRISPR/Cas9-targeted mutagenesis in the Colorado potato beetle, Leptinotarsa decemlineata" @default.
• W2999851567 cites W1509976803 @default.
• W2999851567 cites W1571204959 @default.
• W2999851567 cites W1968342623 @default.
• W2999851567 cites W1976391205 @default.
• W2999851567 cites W2010677471 @default.
• W2999851567 cites W2045435533 @default.
• W2999851567 cites W2075272557 @default.
• W2999851567 cites W2122941722 @default.
• W2999851567 cites W2128412621 @default.
• W2999851567 cites W2138581811 @default.
• W2999851567 cites W2141204618 @default.
• W2999851567 cites W2143427287 @default.
• W2999851567 cites W2190054909 @default.
• W2999851567 cites W2236909218 @default.
• W2999851567 cites W2294164036 @default.
• W2999851567 cites W2328089406 @default.
• W2999851567 cites W2342648418 @default.
• W2999851567 cites W2530970859 @default.
• W2999851567 cites W2578910048 @default.
• W2999851567 cites W2579789675 @default.
• W2999851567 cites W2752815872 @default.
• W2999851567 cites W2760275602 @default.
• W2999851567 cites W2783299198 @default.
• W2999851567 cites W2789757256 @default.
• W2999851567 cites W2794267795 @default.
• W2999851567 cites W2886008440 @default.
• W2999851567 cites W2891027664 @default.
• W2999851567 cites W2901122574 @default.
• W2999851567 cites W2935319699 @default.
• W2999851567 cites W2937747363 @default.
• W2999851567 doi "https://doi.org/10.1016/j.jinsphys.2020.104013" @default.
• W2999851567 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/31917244" @default.
• W2999851567 hasPublicationYear "2020" @default.
• W2999851567 type Work @default.
• W2999851567 sameAs 2999851567 @default.
• W2999851567 citedByCount "21" @default.
• W2999851567 countsByYear W29998515672020 @default.
• W2999851567 countsByYear W29998515672021 @default.
• W2999851567 countsByYear W29998515672022 @default.
• W2999851567 crossrefType "journal-article" @default.
• W2999851567 hasAuthorship W2999851567A5034229389 @default.
• W2999851567 hasAuthorship W2999851567A5065087222 @default.
• W2999851567 hasAuthorship W2999851567A5066515722 @default.
• W2999851567 hasAuthorship W2999851567A5089906704 @default.
• W2999851567 hasConcept C104317684 @default.
• W2999851567 hasConcept C105795698 @default.
• W2999851567 hasConcept C127716648 @default.
• W2999851567 hasConcept C132455925 @default.
• W2999851567 hasConcept C144501496 @default.
• W2999851567 hasConcept C16318435 @default.
• W2999851567 hasConcept C173758957 @default.
• W2999851567 hasConcept C22508944 @default.
• W2999851567 hasConcept C2777266796 @default.
• W2999851567 hasConcept C2777736838 @default.
• W2999851567 hasConcept C2779707326 @default.
• W2999851567 hasConcept C33923547 @default.
• W2999851567 hasConcept C37536845 @default.
• W2999851567 hasConcept C501734568 @default.
• W2999851567 hasConcept C54355233 @default.
• W2999851567 hasConcept C59822182 @default.
• W2999851567 hasConcept C70721500 @default.
• W2999851567 hasConcept C86803240 @default.
• W2999851567 hasConcept C98108389 @default.
• W2999851567 hasConceptScore W2999851567C104317684 @default.
• W2999851567 hasConceptScore W2999851567C105795698 @default.
• W2999851567 hasConceptScore W2999851567C127716648 @default.
• W2999851567 hasConceptScore W2999851567C132455925 @default.
• W2999851567 hasConceptScore W2999851567C144501496 @default.
• W2999851567 hasConceptScore W2999851567C16318435 @default.
• W2999851567 hasConceptScore W2999851567C173758957 @default.
• W2999851567 hasConceptScore W2999851567C22508944 @default.
• W2999851567 hasConceptScore W2999851567C2777266796 @default.
• W2999851567 hasConceptScore W2999851567C2777736838 @default.
• W2999851567 hasConceptScore W2999851567C2779707326 @default.
• W2999851567 hasConceptScore W2999851567C33923547 @default.
• W2999851567 hasConceptScore W2999851567C37536845 @default.
• W2999851567 hasConceptScore W2999851567C501734568 @default.
• W2999851567 hasConceptScore W2999851567C54355233 @default.
• W2999851567 hasConceptScore W2999851567C59822182 @default.
• W2999851567 hasConceptScore W2999851567C70721500 @default.
• W2999851567 hasConceptScore W2999851567C86803240 @default.
• W2999851567 hasConceptScore W2999851567C98108389 @default.
• W2999851567 hasFunder F4320321730 @default.
• W2999851567 hasFunder F4320322603 @default.
• W2999851567 hasLocation W29998515671 @default.
• W2999851567 hasOpenAccess W2999851567 @default.
• W2999851567 hasPrimaryLocation W29998515671 @default.
• W2999851567 hasRelatedWork W2134268326 @default.
• W2999851567 hasRelatedWork W2527347512 @default.

• next