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- W3000103485 abstract "Within 24 days of cultivation, the ratio of the number of potato microspores at different stages of development was studied depending on the content of 6-BA in nutrient media. Four media options were used: No. 1 MS + 6-BA 1 μM, No. 2 MS + 6-BA 2 μM, No. 3 MS + 6-BA 3 μM, No. 4 MS + 6-BA 4 μM. It was found that the use of MS-based culture media with 1-4 μM 6-BA is effective for the induction of embryoidogenesis in potatoes in microspore culture. 88.0-95.0% of the microspores were in a living state for 24 days. The frequency of embryoid formation in the experiment was 0.5-1.0% of the total number of microspores and 4.7-6.6% of the number of multicellular structures. Monad could be the initial cells of the embryoids of the tetraploid variety of potato in the period up to 11-12 days. In addition to single germ-like structures, in the suspension of potato microspores, embryoid conglomerates - polyembrioids - were noted on days 11-12. In the light, microspores and germ-like structures of Nevsky tetraploid potato variety acquired a green color. On the 24th day of cultivation, developing potato embryoids were detected in the phase of organogenesis." @default.
- W3000103485 created "2020-01-23" @default.
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- W3000103485 date "2020-01-07" @default.
- W3000103485 modified "2023-09-24" @default.
- W3000103485 title "Culture of potato microspores (Solanum tuberosum L.) Nevsky variety" @default.
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- W3000103485 doi "https://doi.org/10.1088/1755-1315/421/2/022048" @default.
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