Matches in SemOpenAlex for { <https://semopenalex.org/work/W3000106463> ?p ?o ?g. }
Showing items 1 to 96 of
96
with 100 items per page.
- W3000106463 endingPage "291" @default.
- W3000106463 startingPage "280" @default.
- W3000106463 abstract "Potential utility of 14 candidate housekeeping genes as normalization reference for RT-qPCR analysis with developmental samples (fertilized eggs to late veliger larvae) in Pacific abalone Haliotis discus hannai was evaluated using four different statistical algorithms (geNorm, NormFinder, BestKeeper and comparative ΔCT method). Different algorithms identified different genes as the best candidates, and geometric mean-based final ranking from the most to the least stable expression was as follow: RPL5, RPL4, RPS18, RPL8, RPL7, UBE2, RPL7A, GAPDH, RPL36, PPIB, EF1A, ACTB and B-TU. The findings were further validated via relative quantification of metallothionein (MT) transcripts using the stable and unstable reference genes, and expression levels of MT were greatly influenced according to the choice of reference genes. In overall, our data suggest that RPL5 and RPS18, either singly or in combination, are appropriate for normalizing gene expression in developmental samples of this abalone species, whereas ACTB, B-TU and EF1A are less stable and not recommended. In addition, our findings propose that standard deviations in geometric ranking as well as geometric mean itself should also be taken into account for the final selection of reference gene(s). This study could be a useful basis to facilitate the generation of accurate and reliable RT-qPCR data with developmental samples in this abalone species." @default.
- W3000106463 created "2020-01-23" @default.
- W3000106463 creator A5061456230 @default.
- W3000106463 creator A5068462743 @default.
- W3000106463 creator A5069596946 @default.
- W3000106463 date "2019-12-31" @default.
- W3000106463 modified "2023-09-26" @default.
- W3000106463 title "Assessment of Suitable Reference Genes for RT-qPCR Normalization with Developmental Samples in Pacific Abalone <i>Haliotis discus hannai</i>" @default.
- W3000106463 cites W1577577364 @default.
- W3000106463 cites W17588068 @default.
- W3000106463 cites W1980469442 @default.
- W3000106463 cites W1984454613 @default.
- W3000106463 cites W1985569492 @default.
- W3000106463 cites W1986080495 @default.
- W3000106463 cites W1988334089 @default.
- W3000106463 cites W2008097708 @default.
- W3000106463 cites W2012984422 @default.
- W3000106463 cites W2016751415 @default.
- W3000106463 cites W2035963479 @default.
- W3000106463 cites W2038309605 @default.
- W3000106463 cites W2047346590 @default.
- W3000106463 cites W2056573980 @default.
- W3000106463 cites W2062741288 @default.
- W3000106463 cites W2062938054 @default.
- W3000106463 cites W2065589980 @default.
- W3000106463 cites W2098218231 @default.
- W3000106463 cites W2099824652 @default.
- W3000106463 cites W2105423509 @default.
- W3000106463 cites W2107511340 @default.
- W3000106463 cites W2108068289 @default.
- W3000106463 cites W2110885661 @default.
- W3000106463 cites W2111427334 @default.
- W3000106463 cites W2114570899 @default.
- W3000106463 cites W2123436811 @default.
- W3000106463 cites W2138581811 @default.
- W3000106463 cites W2152544605 @default.
- W3000106463 cites W2159769224 @default.
- W3000106463 cites W2168420558 @default.
- W3000106463 cites W2395916326 @default.
- W3000106463 cites W2416333224 @default.
- W3000106463 cites W2465104623 @default.
- W3000106463 cites W2526248337 @default.
- W3000106463 cites W2605897418 @default.
- W3000106463 cites W2620312173 @default.
- W3000106463 cites W2620521047 @default.
- W3000106463 cites W2753769089 @default.
- W3000106463 cites W2788059668 @default.
- W3000106463 doi "https://doi.org/10.12750/jarb.34.4.280" @default.
- W3000106463 hasPublicationYear "2019" @default.
- W3000106463 type Work @default.
- W3000106463 sameAs 3000106463 @default.
- W3000106463 citedByCount "3" @default.
- W3000106463 countsByYear W30001064632020 @default.
- W3000106463 countsByYear W30001064632022 @default.
- W3000106463 crossrefType "journal-article" @default.
- W3000106463 hasAuthorship W3000106463A5061456230 @default.
- W3000106463 hasAuthorship W3000106463A5068462743 @default.
- W3000106463 hasAuthorship W3000106463A5069596946 @default.
- W3000106463 hasBestOaLocation W30001064631 @default.
- W3000106463 hasConcept C136886441 @default.
- W3000106463 hasConcept C144024400 @default.
- W3000106463 hasConcept C19165224 @default.
- W3000106463 hasConcept C2775933076 @default.
- W3000106463 hasConcept C2780982499 @default.
- W3000106463 hasConcept C505870484 @default.
- W3000106463 hasConcept C86803240 @default.
- W3000106463 hasConcept C90856448 @default.
- W3000106463 hasConceptScore W3000106463C136886441 @default.
- W3000106463 hasConceptScore W3000106463C144024400 @default.
- W3000106463 hasConceptScore W3000106463C19165224 @default.
- W3000106463 hasConceptScore W3000106463C2775933076 @default.
- W3000106463 hasConceptScore W3000106463C2780982499 @default.
- W3000106463 hasConceptScore W3000106463C505870484 @default.
- W3000106463 hasConceptScore W3000106463C86803240 @default.
- W3000106463 hasConceptScore W3000106463C90856448 @default.
- W3000106463 hasIssue "4" @default.
- W3000106463 hasLocation W30001064631 @default.
- W3000106463 hasOpenAccess W3000106463 @default.
- W3000106463 hasPrimaryLocation W30001064631 @default.
- W3000106463 hasRelatedWork W2021940961 @default.
- W3000106463 hasRelatedWork W2049136832 @default.
- W3000106463 hasRelatedWork W2343007015 @default.
- W3000106463 hasRelatedWork W2508734376 @default.
- W3000106463 hasRelatedWork W2516175129 @default.
- W3000106463 hasRelatedWork W2567294345 @default.
- W3000106463 hasRelatedWork W2589065925 @default.
- W3000106463 hasRelatedWork W2810323970 @default.
- W3000106463 hasRelatedWork W3000106463 @default.
- W3000106463 hasRelatedWork W3095887116 @default.
- W3000106463 hasVolume "34" @default.
- W3000106463 isParatext "false" @default.
- W3000106463 isRetracted "false" @default.
- W3000106463 magId "3000106463" @default.
- W3000106463 workType "article" @default.