FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W3000257907> ?p ?o ?g. }

• W3000257907 endingPage "e8254" @default.
• W3000257907 startingPage "e8254" @default.
• W3000257907 abstract "The aim of this study was to find out neuron (-like) cells in peripheral organs by cell markers in rats. Adult male Sprague-Dawley rats were anaesthetized. Their organs including brain, heart, lung, liver, kidney, stomach, duodenum, and ileum were harvested. The mRNA and protein in these organs were extracted. RNA sequencing (RNA-Seq) was carried out, and NeuN, a specific marker for neuronal soma, was assayed with Western blotting. The sections of the aforementioned organs were obtained after a routine fixation (4% methanal)-dehydration (ethanol)-embedding (paraffin) process. NeuN in the sections and seven non-neuronal cell lines was analyzed by immunofluorescence (IF) or immunohistochemistry (IHC). Neuronal markers, such as Eno2, NeuN (Rbfox3), choline acetyltransferase (Chat), as well as tyrosine hydroxylase (Th), and neuronal-glial markers, e.g., glial fibrillary acidic protein (Gfap), S100b, 2', 3'-cyclic nucleotide 3'-phosphodiesterase (Cnp), and other related markers, were positively expressed in all the organs at mRNA level. NeuN was further analyzed by Western blotting. The IF and IHC assays showed that NeuN-positive cells were distributed in all the peripheral tissues (mainly peri-nuclear NeuN-positive cells) though with different patterns from that in brain (nuclear NeuN-positive cells), and a NeuN-negative tissue could not be found. Especially, NeuN and Myl3 co-expressed in the cytoplasm of myocardial cells, suggesting that NeuN could possess other functions than neuronal differentiation. Also, the protein was positively expressed in seven non-neuronal cell lines. Our findings suggested that NeuN-positive cells exist widely, and without identification of its distribution pattern, the specificity of NeuN for neurons could be limited." @default.
• W3000257907 created "2020-01-23" @default.
• W3000257907 creator A5006920853 @default.
• W3000257907 creator A5009318195 @default.
• W3000257907 creator A5013261650 @default.
• W3000257907 creator A5041530586 @default.
• W3000257907 creator A5053969245 @default.
• W3000257907 creator A5062938317 @default.
• W3000257907 date "2020-01-07" @default.
• W3000257907 modified "2023-09-30" @default.
• W3000257907 title "A pilot study on searching for peri-nuclear NeuN-positive cells" @default.
• W3000257907 cites W141279955 @default.
• W3000257907 cites W1512296491 @default.
• W3000257907 cites W177683657 @default.
• W3000257907 cites W1966667764 @default.
• W3000257907 cites W1972481643 @default.
• W3000257907 cites W1978296952 @default.
• W3000257907 cites W1992120851 @default.
• W3000257907 cites W1994087800 @default.
• W3000257907 cites W2004444318 @default.
• W3000257907 cites W2005618242 @default.
• W3000257907 cites W2005814409 @default.
• W3000257907 cites W2010841889 @default.
• W3000257907 cites W2039372333 @default.
• W3000257907 cites W2039648313 @default.
• W3000257907 cites W2086878091 @default.
• W3000257907 cites W2088745158 @default.
• W3000257907 cites W2089227350 @default.
• W3000257907 cites W2094575596 @default.
• W3000257907 cites W2124169580 @default.
• W3000257907 cites W2132691381 @default.
• W3000257907 cites W2141458291 @default.
• W3000257907 cites W2150262642 @default.
• W3000257907 cites W2157935084 @default.
• W3000257907 cites W2158496649 @default.
• W3000257907 cites W2282091730 @default.
• W3000257907 cites W2520141813 @default.
• W3000257907 cites W2524261334 @default.
• W3000257907 cites W2526584921 @default.
• W3000257907 cites W2581259096 @default.
• W3000257907 cites W2620796881 @default.
• W3000257907 cites W2743360159 @default.
• W3000257907 cites W2892292172 @default.
• W3000257907 cites W2893868684 @default.
• W3000257907 cites W2895381526 @default.
• W3000257907 cites W2896097526 @default.
• W3000257907 cites W2911391308 @default.
• W3000257907 cites W2952459265 @default.
• W3000257907 cites W68962965 @default.
• W3000257907 doi "https://doi.org/10.7717/peerj.8254" @default.
• W3000257907 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/6953339" @default.
• W3000257907 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/31938576" @default.
• W3000257907 hasPublicationYear "2020" @default.
• W3000257907 type Work @default.
• W3000257907 sameAs 3000257907 @default.
• W3000257907 citedByCount "4" @default.
• W3000257907 countsByYear W30002579072020 @default.
• W3000257907 countsByYear W30002579072021 @default.
• W3000257907 countsByYear W30002579072023 @default.
• W3000257907 crossrefType "journal-article" @default.
• W3000257907 hasAuthorship W3000257907A5006920853 @default.
• W3000257907 hasAuthorship W3000257907A5009318195 @default.
• W3000257907 hasAuthorship W3000257907A5013261650 @default.
• W3000257907 hasAuthorship W3000257907A5041530586 @default.
• W3000257907 hasAuthorship W3000257907A5053969245 @default.
• W3000257907 hasAuthorship W3000257907A5062938317 @default.
• W3000257907 hasBestOaLocation W30002579071 @default.
• W3000257907 hasConcept C104317684 @default.
• W3000257907 hasConcept C105580179 @default.
• W3000257907 hasConcept C142724271 @default.
• W3000257907 hasConcept C153911025 @default.
• W3000257907 hasConcept C203014093 @default.
• W3000257907 hasConcept C204232928 @default.
• W3000257907 hasConcept C2780495277 @default.
• W3000257907 hasConcept C2781100881 @default.
• W3000257907 hasConcept C37753355 @default.
• W3000257907 hasConcept C54355233 @default.
• W3000257907 hasConcept C55493867 @default.
• W3000257907 hasConcept C71924100 @default.
• W3000257907 hasConcept C74864618 @default.
• W3000257907 hasConcept C81439078 @default.
• W3000257907 hasConcept C85265743 @default.
• W3000257907 hasConcept C86803240 @default.
• W3000257907 hasConcept C95444343 @default.
• W3000257907 hasConceptScore W3000257907C104317684 @default.
• W3000257907 hasConceptScore W3000257907C105580179 @default.
• W3000257907 hasConceptScore W3000257907C142724271 @default.
• W3000257907 hasConceptScore W3000257907C153911025 @default.
• W3000257907 hasConceptScore W3000257907C203014093 @default.
• W3000257907 hasConceptScore W3000257907C204232928 @default.
• W3000257907 hasConceptScore W3000257907C2780495277 @default.
• W3000257907 hasConceptScore W3000257907C2781100881 @default.
• W3000257907 hasConceptScore W3000257907C37753355 @default.
• W3000257907 hasConceptScore W3000257907C54355233 @default.
• W3000257907 hasConceptScore W3000257907C55493867 @default.
• W3000257907 hasConceptScore W3000257907C71924100 @default.
• W3000257907 hasConceptScore W3000257907C74864618 @default.
• W3000257907 hasConceptScore W3000257907C81439078 @default.

• next