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• W3000291559 startingPage "e0227648" @default.
• W3000291559 abstract "Sample collection, processing, storage and isolation methods constitute pre-analytic factors that can influence the quality of samples used in research and clinical practice. With regard to biobanking practices, a critical point in the sample’s life chain is storage, particularly long-term storage. Since most studies examine the influence of different temperatures (4°C, room temperature) or delays in sample processing on sample quality, there is only little information on the effects of long-term storage at ultra-low (vapor phase of liquid nitrogen) temperatures on biomarker levels. Among these biomarkers, circulating miRNAs hold great potential for diagnosis or prognosis for a variety of diseases, like cancer, infections and chronic diseases, and are thus of high interest in several scientific questions. We therefore investigated the influence of long-term storage on levels of eight circulating miRNAs (miR-103a-3p, miR-191-5p, miR-124-3p, miR-30c-5p, miR-451a, miR-23a-3p, miR-93-5p, miR-24-3p, and miR-33b-5p) from 10 participants from the population-based cohort study KORA. Sample collection took place during the baseline survey S4 and the follow-up surveys F4 and FF4, over a time period spanning from 1999 to 2014. The influence of freeze-thaw (f/t) cycles on miRNA stability was also investigated using samples from volunteers (n = 6). Obtained plasma samples were profiled using Exiqon’s miRCURYTM real-time PCR profiling system, and repeated measures ANOVA was used to check for storage or f/t effects. Our results show that detected levels of most of the studied miRNAs showed no statistically significant changes due to storage at ultra-low temperatures for up to 17 years; miR-451a levels were altered due to contamination during sampling. Freeze-thawing of one to four cycles showed an effect only on miR-30c-5p. Our results highlight the robustness of this set of circulating miRNAs for decades of storage at ultra-low temperatures and several freeze-thaw cycles, which makes our findings increasingly relevant for research conducted with biobanked samples." @default.
• W3000291559 created "2020-01-23" @default.
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• W3000291559 date "2020-01-14" @default.
• W3000291559 modified "2023-10-03" @default.
• W3000291559 title "Impact of long-term storage and freeze-thawing on eight circulating microRNAs in plasma samples" @default.
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• W3000291559 doi "https://doi.org/10.1371/journal.pone.0227648" @default.
• W3000291559 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/6959605" @default.
• W3000291559 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/31935258" @default.
• W3000291559 hasPublicationYear "2020" @default.
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