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• W3000478214 abstract "We describe a technique for retrograde labeling of motor neurons in Drosophila. We use an oil-dissolved lipophilic dye and deliver a small droplet to an embryonic fillet preparation by a microinjector. Each motor neuron whose membrane is contacted by the droplet can then be rapidly labeled. Individual motor neurons are continuously labeled, enabling fine structural details to be clearly visualized. Given that lipophilic dyes come in various colors, the technique also provides a means to get adjacent neurons labeled in multicolor. This tracing technique is therefore useful for studying neuronal morphogenesis and synaptic connectivity in the motor neuron system of Drosophila." @default.
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• W3000478214 date "2020-01-12" @default.
• W3000478214 modified "2023-10-18" @default.
• W3000478214 title "Retrograde Tracing of <em>Drosophila</em> Embryonic Motor Neurons Using Lipophilic Fluorescent Dyes" @default.
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• W3000478214 doi "https://doi.org/10.3791/60716" @default.
• W3000478214 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/7315430" @default.
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