FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W3000568241> ?p ?o ?g. }

• W3000568241 abstract "Abstract A functional primary cilium is essential for normal and regulated signalling. Primary ciliary defects cause a group of developmental conditions known as ciliopathies, but the precise mechanisms of signal regulation by the cilium remain unclear. Previous studies have implicated the ubiquitin proteasome system (UPS) in regulation of Wnt signalling at the ciliary basal body. Here, we provide mechanistic insight into ciliary ubiquitin processing in cells and for a ciliopathy mouse model lacking the ciliary protein Mks1. In vivo loss of Mks1 sensitizes cells to proteasomal disruption, leading to abnormal accumulation of ubiquitinated proteins. To substantiate a direct link between MKS1 and the UPS, we identified UBE2E1, an E2 ubiquitin-conjugating enzyme that polyubiquitinates β-catenin, and RNF34, an E3 ligase, as novel interactants of MKS1. UBE2E1 and MKS1 colocalized, particularly during conditions of ciliary resorption, and loss of UBE2E1 recapitulates the ciliary and Wnt signalling phenotypes observed during loss of MKS1. Levels of UBE2E1 and MKS1 are co-dependent and UBE2E1 mediates both regulatory and degradative ubiquitination of MKS1. Furthermore, we demonstrate that processing of phosphorylated β-catenin occurs at the ciliary base through the functional interaction between UBE2E1 and MKS1. These observations suggest that correct β-catenin levels are tightly regulated at the primary cilium by a ciliary-specific E2 (UBE2E1) and a regulatory substrate-adaptor (MKS1), confirming the fundamental role of UPS defects in the molecular pathogenesis of ciliopathies." @default.
• W3000568241 created "2020-01-23" @default.
• W3000568241 creator A5004279317 @default.
• W3000568241 creator A5026017797 @default.
• W3000568241 creator A5029132133 @default.
• W3000568241 creator A5033253597 @default.
• W3000568241 creator A5037360441 @default.
• W3000568241 creator A5053204551 @default.
• W3000568241 creator A5066199693 @default.
• W3000568241 creator A5077941525 @default.
• W3000568241 creator A5081837333 @default.
• W3000568241 date "2020-01-09" @default.
• W3000568241 modified "2023-10-15" @default.
• W3000568241 title "Regulation of canonical Wnt signalling by the ciliopathy protein MKS1 and the E2 ubiquitin-conjugating enzyme UBE2E1" @default.
• W3000568241 cites W116831975 @default.
• W3000568241 cites W1532810559 @default.
• W3000568241 cites W1589408448 @default.
• W3000568241 cites W1967921405 @default.
• W3000568241 cites W1969717239 @default.
• W3000568241 cites W1974601240 @default.
• W3000568241 cites W1979521719 @default.
• W3000568241 cites W1985225356 @default.
• W3000568241 cites W1986170218 @default.
• W3000568241 cites W1987529702 @default.
• W3000568241 cites W1990020901 @default.
• W3000568241 cites W1992816628 @default.
• W3000568241 cites W1998590298 @default.
• W3000568241 cites W2009877599 @default.
• W3000568241 cites W2018490222 @default.
• W3000568241 cites W2028607959 @default.
• W3000568241 cites W2038678925 @default.
• W3000568241 cites W2039661491 @default.
• W3000568241 cites W2040930425 @default.
• W3000568241 cites W2041964741 @default.
• W3000568241 cites W2056696934 @default.
• W3000568241 cites W2057254962 @default.
• W3000568241 cites W2062186455 @default.
• W3000568241 cites W2063293509 @default.
• W3000568241 cites W2064394604 @default.
• W3000568241 cites W2073777182 @default.
• W3000568241 cites W2074314807 @default.
• W3000568241 cites W2081985759 @default.
• W3000568241 cites W2082387343 @default.
• W3000568241 cites W2083904901 @default.
• W3000568241 cites W2091684557 @default.
• W3000568241 cites W2094856298 @default.
• W3000568241 cites W2095869636 @default.
• W3000568241 cites W2095963321 @default.
• W3000568241 cites W2102864788 @default.
• W3000568241 cites W2113283367 @default.
• W3000568241 cites W2114810168 @default.
• W3000568241 cites W2114873132 @default.
• W3000568241 cites W2120858746 @default.
• W3000568241 cites W2127740472 @default.
• W3000568241 cites W2130801954 @default.
• W3000568241 cites W2132045816 @default.
• W3000568241 cites W2132325184 @default.
• W3000568241 cites W2132475598 @default.
• W3000568241 cites W2134709687 @default.
• W3000568241 cites W2136391236 @default.
• W3000568241 cites W2144015228 @default.
• W3000568241 cites W2147791710 @default.
• W3000568241 cites W2152007993 @default.
• W3000568241 cites W2161870393 @default.
• W3000568241 cites W2171608919 @default.
• W3000568241 cites W2188873428 @default.
• W3000568241 cites W2309366384 @default.
• W3000568241 cites W2348394504 @default.
• W3000568241 cites W2928892233 @default.
• W3000568241 doi "https://doi.org/10.1101/2020.01.08.897959" @default.
• W3000568241 hasPublicationYear "2020" @default.
• W3000568241 type Work @default.
• W3000568241 sameAs 3000568241 @default.
• W3000568241 citedByCount "0" @default.
• W3000568241 crossrefType "posted-content" @default.
• W3000568241 hasAuthorship W3000568241A5004279317 @default.
• W3000568241 hasAuthorship W3000568241A5026017797 @default.
• W3000568241 hasAuthorship W3000568241A5029132133 @default.
• W3000568241 hasAuthorship W3000568241A5033253597 @default.
• W3000568241 hasAuthorship W3000568241A5037360441 @default.
• W3000568241 hasAuthorship W3000568241A5053204551 @default.
• W3000568241 hasAuthorship W3000568241A5066199693 @default.
• W3000568241 hasAuthorship W3000568241A5077941525 @default.
• W3000568241 hasAuthorship W3000568241A5081837333 @default.
• W3000568241 hasBestOaLocation W30005682411 @default.
• W3000568241 hasConcept C100094513 @default.
• W3000568241 hasConcept C104317684 @default.
• W3000568241 hasConcept C127716648 @default.
• W3000568241 hasConcept C25602115 @default.
• W3000568241 hasConcept C2780561973 @default.
• W3000568241 hasConcept C2910395002 @default.
• W3000568241 hasConcept C54355233 @default.
• W3000568241 hasConcept C86803240 @default.
• W3000568241 hasConcept C95444343 @default.
• W3000568241 hasConceptScore W3000568241C100094513 @default.
• W3000568241 hasConceptScore W3000568241C104317684 @default.
• W3000568241 hasConceptScore W3000568241C127716648 @default.
• W3000568241 hasConceptScore W3000568241C25602115 @default.
• W3000568241 hasConceptScore W3000568241C2780561973 @default.
• W3000568241 hasConceptScore W3000568241C2910395002 @default.

• next