FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W3000656492> ?p ?o ?g. }

• W3000656492 abstract "BACKGROUND:Ischemia-reperfusion injury (IRI) results from the restoration of blood supply to ischemic organs, including the heart. Expression of microRNA-668-3p (miR-668-3p) is known to protect the kidney from IRI. This study aimed to investigate the role of miR-668-3p in oxygen-glucose deprivation (OGD) in a rat H9c2 cardiomyocyte model of IRI. MATERIAL AND METHODS:Rat H9c2 cardiomyocytes were cultured in glucose-free Dulbecco’s modified Eagle’s medium (DMEM) under anaerobic conditions, followed by oxygenation, to create the OGD model of IRI. The luciferase reporter assay evaluated the interaction between stromal cell-derived factor-1 (SDF-1), or CXC motif chemokine 12 (CXCL12), and miR-668-3p. Protein and mRNA levels of SDF-1, CXCR4, Bcl2, Bax, cleaved caspase-3, endothelial nitric oxide synthase (eNOS), and phosphorylated endothelial nitric oxide synthase (p-eNOS) were analyzed by Western blot and quantitative reverse transcription-polymerase chain reaction (RT-qPCR), and apoptosis were assessed by flow cytometry. Enzyme-linked immunosorbent assay (ELISA) measured reactive oxygen species (ROS), including malondialdehyde (MDA), nitric oxide (NO), p-eNOS, and the inflammatory cytokines, tumor necrosis factor-α (TNF-α), interleukin-1β (IL-1β), IL-6, and monocyte chemoattractant protein-1 (MCP-1) in H9c2 cell supernatants. RESULTS:In the OGD rat H9c2 cardiomyocyte model of IRI, miR-668-3p levels were reduced. Overexpression of miR-668-3p inhibited SDF-1, CXCR4, the expression of inflammatory cytokines, markers of oxidative stress, and p-eNOS. The overexpression of SDF-1 reversed these findings. Overexpression of SDF-1 promoted cell apoptosis, which was reduced by miR-668-3p. CONCLUSIONS:In the OGD rat H9c2 cardiomyocyte model of IRI, miR-668-3p suppressed mediators of inflammation and oxidative stress and enhanced cell viability through the SDF-1/CXCR4 signaling pathway." @default.
• W3000656492 created "2020-01-23" @default.
• W3000656492 creator A5000665985 @default.
• W3000656492 creator A5010675918 @default.
• W3000656492 creator A5066654480 @default.
• W3000656492 date "2020-01-17" @default.
• W3000656492 modified "2023-10-14" @default.
• W3000656492 title "MicroRNA-668-3p Protects Against Oxygen-Glucose Deprivation in a Rat H9c2 Cardiomyocyte Model of Ischemia-Reperfusion Injury by Targeting the Stromal Cell-Derived Factor-1 (SDF-1)/CXCR4 Signaling Pathway" @default.
• W3000656492 cites W1976616659 @default.
• W3000656492 cites W2002189205 @default.
• W3000656492 cites W2016087073 @default.
• W3000656492 cites W2033178653 @default.
• W3000656492 cites W2087126389 @default.
• W3000656492 cites W2109698138 @default.
• W3000656492 cites W2147330933 @default.
• W3000656492 cites W2169252817 @default.
• W3000656492 cites W2253797991 @default.
• W3000656492 cites W2334701308 @default.
• W3000656492 cites W2516271526 @default.
• W3000656492 cites W2578977077 @default.
• W3000656492 cites W2761109619 @default.
• W3000656492 cites W2772036482 @default.
• W3000656492 cites W2772713290 @default.
• W3000656492 cites W2783731118 @default.
• W3000656492 cites W2790485200 @default.
• W3000656492 cites W2793851406 @default.
• W3000656492 cites W2893560105 @default.
• W3000656492 cites W2895895706 @default.
• W3000656492 cites W2899889571 @default.
• W3000656492 cites W2903716282 @default.
• W3000656492 cites W2944084395 @default.
• W3000656492 cites W2948178155 @default.
• W3000656492 cites W2954053661 @default.
• W3000656492 cites W2954448622 @default.
• W3000656492 cites W2954881901 @default.
• W3000656492 cites W2957267978 @default.
• W3000656492 cites W2961692773 @default.
• W3000656492 cites W2966693829 @default.
• W3000656492 cites W2981782890 @default.
• W3000656492 cites W2993449680 @default.
• W3000656492 cites W3024368199 @default.
• W3000656492 doi "https://doi.org/10.12659/msm.919601" @default.
• W3000656492 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/7003666" @default.
• W3000656492 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/31997826" @default.
• W3000656492 hasPublicationYear "2020" @default.
• W3000656492 type Work @default.
• W3000656492 sameAs 3000656492 @default.
• W3000656492 citedByCount "8" @default.
• W3000656492 countsByYear W30006564922021 @default.
• W3000656492 countsByYear W30006564922022 @default.
• W3000656492 countsByYear W30006564922023 @default.
• W3000656492 crossrefType "journal-article" @default.
• W3000656492 hasAuthorship W3000656492A5000665985 @default.
• W3000656492 hasAuthorship W3000656492A5010675918 @default.
• W3000656492 hasAuthorship W3000656492A5066654480 @default.
• W3000656492 hasBestOaLocation W30006564922 @default.
• W3000656492 hasConcept C126322002 @default.
• W3000656492 hasConcept C134018914 @default.
• W3000656492 hasConcept C153911025 @default.
• W3000656492 hasConcept C16930146 @default.
• W3000656492 hasConcept C185592680 @default.
• W3000656492 hasConcept C2777622882 @default.
• W3000656492 hasConcept C2778326061 @default.
• W3000656492 hasConcept C2780114680 @default.
• W3000656492 hasConcept C502942594 @default.
• W3000656492 hasConcept C519581460 @default.
• W3000656492 hasConcept C541997718 @default.
• W3000656492 hasConcept C71924100 @default.
• W3000656492 hasConcept C86803240 @default.
• W3000656492 hasConcept C98274493 @default.
• W3000656492 hasConceptScore W3000656492C126322002 @default.
• W3000656492 hasConceptScore W3000656492C134018914 @default.
• W3000656492 hasConceptScore W3000656492C153911025 @default.
• W3000656492 hasConceptScore W3000656492C16930146 @default.
• W3000656492 hasConceptScore W3000656492C185592680 @default.
• W3000656492 hasConceptScore W3000656492C2777622882 @default.
• W3000656492 hasConceptScore W3000656492C2778326061 @default.
• W3000656492 hasConceptScore W3000656492C2780114680 @default.
• W3000656492 hasConceptScore W3000656492C502942594 @default.
• W3000656492 hasConceptScore W3000656492C519581460 @default.
• W3000656492 hasConceptScore W3000656492C541997718 @default.
• W3000656492 hasConceptScore W3000656492C71924100 @default.
• W3000656492 hasConceptScore W3000656492C86803240 @default.
• W3000656492 hasConceptScore W3000656492C98274493 @default.
• W3000656492 hasLocation W30006564921 @default.
• W3000656492 hasLocation W30006564922 @default.
• W3000656492 hasLocation W30006564923 @default.
• W3000656492 hasOpenAccess W3000656492 @default.
• W3000656492 hasPrimaryLocation W30006564921 @default.
• W3000656492 hasRelatedWork W1500167557 @default.
• W3000656492 hasRelatedWork W1981407854 @default.
• W3000656492 hasRelatedWork W1987269973 @default.
• W3000656492 hasRelatedWork W2030742850 @default.
• W3000656492 hasRelatedWork W2115648058 @default.
• W3000656492 hasRelatedWork W2134382614 @default.
• W3000656492 hasRelatedWork W2289257592 @default.
• W3000656492 hasRelatedWork W2382325930 @default.
• W3000656492 hasRelatedWork W2394313808 @default.
• W3000656492 hasRelatedWork W2395494434 @default.
• W3000656492 hasVolume "26" @default.

• next