FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W3000678493> ?p ?o ?g. }

• W3000678493 abstract "Background and aims : Human immunodeficiency virus type 1 (HIV-1) based-lentivirus vector is one of the most promising viral vectors for gene delivery in different cell lines including gastric cell lines. Therefore, the aim of this study was to produce a lentivirus vector for transduction and expression of green fluorescent protein (GFP) in human gastric cancer cell line, AGS. Materials and Methods: In this piece of work, Escherichia coli HB101 was transformed with plasmids psPAX2, pTD, and pMD2.G, following the purification of which their DNA was extracted along with their quantity and quality evaluated to be used in the next experiments. Subsequently, to produce the vector, the packaging cells were transfected with the plasmids and the vector containing supernatant was collected and purified using ultracentrifuge. ELISA was used to confirm the construction of the vector. Fluorescent microscopy and flow cytometry were used to check the expression of GFP in the cell line and to calculate the percentage of GFP expression, respectively. Results: In this study, the results of ELISA confirmed the construction of the plasmid used in this study. AGS cells were infected with viruses produced to detect the viral activity and GFP expression was evaluated by fluorescence microscopy and flow cytometry after 72 hours. Based on the results of flow cytometry, GFP was expressed in over 90% of transduced AGS cells. Conclusion: The results of this study showed that lentiviral vector is a highly efficient vector for expression of GFP gene in AGS cell line." @default.
• W3000678493 created "2020-01-23" @default.
• W3000678493 creator A5007785852 @default.
• W3000678493 creator A5040921696 @default.
• W3000678493 creator A5066242881 @default.
• W3000678493 creator A5079214244 @default.
• W3000678493 creator A5089365248 @default.
• W3000678493 date "2019-10-30" @default.
• W3000678493 modified "2023-09-28" @default.
• W3000678493 title "Evaluation of lentiviral vector-based green fluorescent protein expression in human gastric cancer cell line" @default.
• W3000678493 cites W1965368778 @default.
• W3000678493 cites W1967503156 @default.
• W3000678493 cites W1970667858 @default.
• W3000678493 cites W1976226754 @default.
• W3000678493 cites W1979235019 @default.
• W3000678493 cites W2001443196 @default.
• W3000678493 cites W2007285603 @default.
• W3000678493 cites W2019514276 @default.
• W3000678493 cites W2041709256 @default.
• W3000678493 cites W2072126111 @default.
• W3000678493 cites W2076011083 @default.
• W3000678493 cites W2078792659 @default.
• W3000678493 cites W2080285163 @default.
• W3000678493 cites W2081369202 @default.
• W3000678493 cites W2083560764 @default.
• W3000678493 cites W2085061750 @default.
• W3000678493 cites W2100349562 @default.
• W3000678493 cites W2125552582 @default.
• W3000678493 cites W2134497088 @default.
• W3000678493 cites W2144828430 @default.
• W3000678493 cites W2151609707 @default.
• W3000678493 cites W2157497892 @default.
• W3000678493 cites W4300487841 @default.
• W3000678493 doi "https://doi.org/10.34172/jsums.2019.36" @default.
• W3000678493 hasPublicationYear "2019" @default.
• W3000678493 type Work @default.
• W3000678493 sameAs 3000678493 @default.
• W3000678493 citedByCount "0" @default.
• W3000678493 crossrefType "journal-article" @default.
• W3000678493 hasAuthorship W3000678493A5007785852 @default.
• W3000678493 hasAuthorship W3000678493A5040921696 @default.
• W3000678493 hasAuthorship W3000678493A5066242881 @default.
• W3000678493 hasAuthorship W3000678493A5079214244 @default.
• W3000678493 hasAuthorship W3000678493A5089365248 @default.
• W3000678493 hasBestOaLocation W30006784931 @default.
• W3000678493 hasConcept C104317684 @default.
• W3000678493 hasConcept C121332964 @default.
• W3000678493 hasConcept C142613039 @default.
• W3000678493 hasConcept C1491633281 @default.
• W3000678493 hasConcept C15152581 @default.
• W3000678493 hasConcept C153911025 @default.
• W3000678493 hasConcept C159047783 @default.
• W3000678493 hasConcept C169274487 @default.
• W3000678493 hasConcept C22744801 @default.
• W3000678493 hasConcept C32470452 @default.
• W3000678493 hasConcept C33161422 @default.
• W3000678493 hasConcept C40767141 @default.
• W3000678493 hasConcept C54009773 @default.
• W3000678493 hasConcept C54355233 @default.
• W3000678493 hasConcept C553184892 @default.
• W3000678493 hasConcept C55493867 @default.
• W3000678493 hasConcept C62520636 @default.
• W3000678493 hasConcept C81885089 @default.
• W3000678493 hasConcept C86803240 @default.
• W3000678493 hasConcept C91881484 @default.
• W3000678493 hasConceptScore W3000678493C104317684 @default.
• W3000678493 hasConceptScore W3000678493C121332964 @default.
• W3000678493 hasConceptScore W3000678493C142613039 @default.
• W3000678493 hasConceptScore W3000678493C1491633281 @default.
• W3000678493 hasConceptScore W3000678493C15152581 @default.
• W3000678493 hasConceptScore W3000678493C153911025 @default.
• W3000678493 hasConceptScore W3000678493C159047783 @default.
• W3000678493 hasConceptScore W3000678493C169274487 @default.
• W3000678493 hasConceptScore W3000678493C22744801 @default.
• W3000678493 hasConceptScore W3000678493C32470452 @default.
• W3000678493 hasConceptScore W3000678493C33161422 @default.
• W3000678493 hasConceptScore W3000678493C40767141 @default.
• W3000678493 hasConceptScore W3000678493C54009773 @default.
• W3000678493 hasConceptScore W3000678493C54355233 @default.
• W3000678493 hasConceptScore W3000678493C553184892 @default.
• W3000678493 hasConceptScore W3000678493C55493867 @default.
• W3000678493 hasConceptScore W3000678493C62520636 @default.
• W3000678493 hasConceptScore W3000678493C81885089 @default.
• W3000678493 hasConceptScore W3000678493C86803240 @default.
• W3000678493 hasConceptScore W3000678493C91881484 @default.
• W3000678493 hasLocation W30006784931 @default.
• W3000678493 hasOpenAccess W3000678493 @default.
• W3000678493 hasPrimaryLocation W30006784931 @default.
• W3000678493 hasRelatedWork W119752022 @default.
• W3000678493 hasRelatedWork W2041452018 @default.
• W3000678493 hasRelatedWork W2072443828 @default.
• W3000678493 hasRelatedWork W2351825726 @default.
• W3000678493 hasRelatedWork W2353192375 @default.
• W3000678493 hasRelatedWork W2353286559 @default.
• W3000678493 hasRelatedWork W2359059053 @default.
• W3000678493 hasRelatedWork W2361303590 @default.
• W3000678493 hasRelatedWork W2368418081 @default.
• W3000678493 hasRelatedWork W2376196438 @default.
• W3000678493 hasRelatedWork W2376644208 @default.
• W3000678493 hasRelatedWork W2384049743 @default.

• next