FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W3000858851> ?p ?o ?g. }

• W3000858851 abstract "Abstract Monoclonal antibodies (mAbs) are attractive therapeutics for treating a wide range of human disorders, and bind to the antigen through their complementarity-determining regions (CDRs). Small stable proteins containing structurally retained CDRs are promising alternatives to mAbs. In this report, we present a method to create such proteins, named fluctuation-regulated affinity proteins (FLAPs). Thirteen graft acceptor (GA) sites that efficiently immobilise the grafted peptide structure were initially selected from six small protein scaffolds by computational identification. Five CDR peptides extracted by binding energy calculations from mAbs against breast cancer marker human epithelial growth factor receptor type 2 (HER2) were then grafted to the selected scaffolds. The combination of five CDR peptides and 13 GA sites in six scaffolds revealed that three of the 65 combinations showed specific binding to HER2 with dissociation constants ( K D ) of 270–350 nM in biolayer interferometry and 24–65 nM in ELISA. The FLAPs specifically detected HER2-overexpressing cancer cells. Thus, the present strategy is a promising and practical method for developing small antibody mimetics." @default.
• W3000858851 created "2020-01-30" @default.
• W3000858851 creator A5010111854 @default.
• W3000858851 creator A5011302743 @default.
• W3000858851 creator A5022881143 @default.
• W3000858851 creator A5025091647 @default.
• W3000858851 creator A5026625455 @default.
• W3000858851 creator A5027082940 @default.
• W3000858851 creator A5035904442 @default.
• W3000858851 creator A5058194652 @default.
• W3000858851 creator A5059930924 @default.
• W3000858851 creator A5063733393 @default.
• W3000858851 creator A5069535251 @default.
• W3000858851 date "2020-01-21" @default.
• W3000858851 modified "2023-10-16" @default.
• W3000858851 title "Design Strategy to Create Antibody Mimetics Harbouring Immobilised Complementarity Determining Region Peptides for Practical Use" @default.
• W3000858851 cites W1529951529 @default.
• W3000858851 cites W1775097118 @default.
• W3000858851 cites W1988537554 @default.
• W3000858851 cites W2013424931 @default.
• W3000858851 cites W2026854113 @default.
• W3000858851 cites W2033447440 @default.
• W3000858851 cites W2051126826 @default.
• W3000858851 cites W2068176347 @default.
• W3000858851 cites W2075097399 @default.
• W3000858851 cites W2081926288 @default.
• W3000858851 cites W2082660881 @default.
• W3000858851 cites W2084417912 @default.
• W3000858851 cites W2092114953 @default.
• W3000858851 cites W2092927147 @default.
• W3000858851 cites W2096836753 @default.
• W3000858851 cites W2105330984 @default.
• W3000858851 cites W2123580257 @default.
• W3000858851 cites W2134450909 @default.
• W3000858851 cites W2136802861 @default.
• W3000858851 cites W2153400235 @default.
• W3000858851 cites W2171223865 @default.
• W3000858851 cites W2332076457 @default.
• W3000858851 cites W2430440408 @default.
• W3000858851 cites W2549181864 @default.
• W3000858851 cites W2605901284 @default.
• W3000858851 cites W2759860792 @default.
• W3000858851 cites W2795688435 @default.
• W3000858851 cites W2897958605 @default.
• W3000858851 cites W2899980190 @default.
• W3000858851 doi "https://doi.org/10.1038/s41598-020-57713-4" @default.
• W3000858851 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/6972867" @default.
• W3000858851 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/31964960" @default.
• W3000858851 hasPublicationYear "2020" @default.
• W3000858851 type Work @default.
• W3000858851 sameAs 3000858851 @default.
• W3000858851 citedByCount "12" @default.
• W3000858851 countsByYear W30008588512020 @default.
• W3000858851 countsByYear W30008588512021 @default.
• W3000858851 countsByYear W30008588512022 @default.
• W3000858851 crossrefType "journal-article" @default.
• W3000858851 hasAuthorship W3000858851A5010111854 @default.
• W3000858851 hasAuthorship W3000858851A5011302743 @default.
• W3000858851 hasAuthorship W3000858851A5022881143 @default.
• W3000858851 hasAuthorship W3000858851A5025091647 @default.
• W3000858851 hasAuthorship W3000858851A5026625455 @default.
• W3000858851 hasAuthorship W3000858851A5027082940 @default.
• W3000858851 hasAuthorship W3000858851A5035904442 @default.
• W3000858851 hasAuthorship W3000858851A5058194652 @default.
• W3000858851 hasAuthorship W3000858851A5059930924 @default.
• W3000858851 hasAuthorship W3000858851A5063733393 @default.
• W3000858851 hasAuthorship W3000858851A5069535251 @default.
• W3000858851 hasBestOaLocation W30008588511 @default.
• W3000858851 hasConcept C147483822 @default.
• W3000858851 hasConcept C153911025 @default.
• W3000858851 hasConcept C159654299 @default.
• W3000858851 hasConcept C170493617 @default.
• W3000858851 hasConcept C185592680 @default.
• W3000858851 hasConcept C203014093 @default.
• W3000858851 hasConcept C21951064 @default.
• W3000858851 hasConcept C2779281246 @default.
• W3000858851 hasConcept C542903549 @default.
• W3000858851 hasConcept C55493867 @default.
• W3000858851 hasConcept C70721500 @default.
• W3000858851 hasConcept C74998103 @default.
• W3000858851 hasConcept C85630698 @default.
• W3000858851 hasConcept C86803240 @default.
• W3000858851 hasConceptScore W3000858851C147483822 @default.
• W3000858851 hasConceptScore W3000858851C153911025 @default.
• W3000858851 hasConceptScore W3000858851C159654299 @default.
• W3000858851 hasConceptScore W3000858851C170493617 @default.
• W3000858851 hasConceptScore W3000858851C185592680 @default.
• W3000858851 hasConceptScore W3000858851C203014093 @default.
• W3000858851 hasConceptScore W3000858851C21951064 @default.
• W3000858851 hasConceptScore W3000858851C2779281246 @default.
• W3000858851 hasConceptScore W3000858851C542903549 @default.
• W3000858851 hasConceptScore W3000858851C55493867 @default.
• W3000858851 hasConceptScore W3000858851C70721500 @default.
• W3000858851 hasConceptScore W3000858851C74998103 @default.
• W3000858851 hasConceptScore W3000858851C85630698 @default.
• W3000858851 hasConceptScore W3000858851C86803240 @default.
• W3000858851 hasFunder F4320311405 @default.
• W3000858851 hasFunder F4320334764 @default.
• W3000858851 hasIssue "1" @default.
• W3000858851 hasLocation W30008588511 @default.

• next