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• W3001047827 startingPage "e0226817" @default.
• W3001047827 abstract "Background A large proportion of neonates are treated for presumed bacterial sepsis with broad spectrum antibiotics even though their blood cultures subsequently show no growth. This study aimed to investigate PCR-based methods to identify pathogens not detected by conventional culture. Methods Whole blood samples of 208 neonates with suspected early onset sepsis were tested using a panel of multiplexed bacterial PCRs targeting Streptococcus pneumoniae, Streptococcus agalactiae (GBS), Staphylococcus aureus, Streptococcus pyogenes (GAS), Enterobacteriaceae, Enterococcus faecalis, Enterococcus faecium, Ureaplasma parvum, Ureaplasma urealyticum, Mycoplasma hominis and Mycoplasma genitalium, a 16S rRNA gene broad-range PCR and a multiplexed PCR for Candida spp. Results Two-hundred and eight samples were processed. In five of those samples, organisms were detected by conventional culture; all of those were also identified by PCR. PCR detected bacteria in 91 (45%) of the 203 samples that did not show bacterial growth in culture. S. aureus, Enterobacteriaceae and S. pneumoniae were the most frequently detected pathogens. A higher bacterial load detected by PCR was correlated positively with the number of clinical signs at presentation. Conclusion Real-time PCR has the potential to be a valuable additional tool for the diagnosis of neonatal sepsis." @default.
• W3001047827 created "2020-01-30" @default.
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• W3001047827 date "2020-01-24" @default.
• W3001047827 modified "2023-10-01" @default.
• W3001047827 title "PCR for the detection of pathogens in neonatal early onset sepsis" @default.
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• W3001047827 doi "https://doi.org/10.1371/journal.pone.0226817" @default.
• W3001047827 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/6980546" @default.
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• W3001047827 hasPublicationYear "2020" @default.
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