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- W3001324918 abstract "Abstract The expression mechanism of permselectivity through a gramicidin A (gA) channel between two aqueous phases (W1 and W2) was investigated. When the concentration of CsCl or CsBr in W1 was equivalent to that in W2, the single‐channel current was proportional to the absolute value of the applied membrane potential. Although the single‐channel current linearly increased with increasing electrolyte concentration in W1 and W2 until about 0.1 M (mol dm −3 ), it began to level off around 0.1 M, indicating that ion permeation across the channel pore is the rate‐determining step and that the saturation of the transporting ion within the channel pore provokes the leveling off. In the case of asymmetric composition of the electrolyte in W1 and W2, the monovalent cation and the counter anion were transported in the opposite direction through the gA channel pore or the bilayer lipid membrane around the gA channel. Finally, the experimental data was fitted using the Goldman‐Hodgkin‐Katz equation based on the relationship between the membrane potential and the single‐channel current to define the ratio of the diffusion coefficients of Cs + , Cl − , and Br − as 5.7 : 1.0 : 0.26." @default.
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- W3001324918 date "2020-01-23" @default.
- W3001324918 modified "2023-09-24" @default.
- W3001324918 title "Permselectivity of Gramicidin A Channels Based on Single‐channel Recordings" @default.
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- W3001324918 doi "https://doi.org/10.1002/elan.201900684" @default.
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