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- W3001688411 abstract "Abstract Reductive alkylation of the carbonyl group of carbohydrates with fluorescence or ionizing labels is a prerequisite for the sensitive analysis of carbohydrates by chromatographic and mass spectrometric techniques. Herein, 1-(2-aminoethyl)-3-methyl-1 H -imidazol-3-ium tetrafluoroborate ([MIEA][BF 4 ]) was successfully synthesized using tert -butyl N -(2-bromoethyl)carbamate and N -methylimidazole as starting materials. MIEA + was then investigated as a multifunctional oligosaccharide label for glycan profiling and identification using LC-ESI-ToF and by MALDI-ToF mass spectrometry. The reductive amination of this diazole with carbohydrates was exemplified by labeling N -glycans from the model glycoproteins horseradish peroxidase, RNase B, and bovine lactoferrin. The produced MIEA + glycan profiles were comparable to the corresponding 2AB labeled glycan derivatives and showed improved ESI-MS ionization efficiency over the respective 2AB derivatives, with detection sensitivity in the low picomol to the high femtomol range." @default.
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- W3001688411 date "2019-08-20" @default.
- W3001688411 modified "2023-10-18" @default.
- W3001688411 title "1-(2-Aminoethyl)-3-methyl-1<i>H</i>-imidazol-3-ium tetrafluoroborate: synthesis and application in carbohydrate analysis" @default.
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- W3001688411 doi "https://doi.org/10.1515/pac-2019-0117" @default.
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