Matches in SemOpenAlex for { <https://semopenalex.org/work/W3001763584> ?p ?o ?g. }
Showing items 1 to 87 of
87
with 100 items per page.
- W3001763584 abstract "ABSTRACT A technical problem of characterizing copy number variation of several cells with next-generation sequencing is the whole genome amplification induced bias. The result of CNVs and mosaicism detection is affected by the GC bias. Here, we report a rapid non-WGA sample preparation strategy for a single-molecule sequencing platform GenoCare1600. This approach, combined with a single-molecule sequencing platform that avoids the use of WGA and bridge PCR processes, can provide higher reliability with its lower GC bias. By combining our optimized Tn5-based transposon insertion approach with GenoCare, we successfully detected CNVs as small as 1.29M and mosaicism as small as 20%, which is consistent with next-generation sequencing (NGS) data. Moreover, our GenoCare-TTI protocol showed less GC bias and less Mad of Diff. These results suggest that the optimized TTI approach, together with the GenoCare1600 sequencing platform, is a promising option for CNV characterization from maybe one single cell." @default.
- W3001763584 created "2020-01-30" @default.
- W3001763584 creator A5010167248 @default.
- W3001763584 creator A5011842501 @default.
- W3001763584 creator A5014182142 @default.
- W3001763584 creator A5028071372 @default.
- W3001763584 creator A5036060606 @default.
- W3001763584 creator A5056977473 @default.
- W3001763584 creator A5074140059 @default.
- W3001763584 creator A5080552339 @default.
- W3001763584 creator A5083858817 @default.
- W3001763584 date "2020-01-23" @default.
- W3001763584 modified "2023-09-27" @default.
- W3001763584 title "Accurate CNV identification from only a few cells with low GC bias in a single-molecule sequencing platform" @default.
- W3001763584 cites W2032518018 @default.
- W3001763584 cites W2039884704 @default.
- W3001763584 cites W2041353701 @default.
- W3001763584 cites W2064977507 @default.
- W3001763584 cites W2069601513 @default.
- W3001763584 cites W2079592358 @default.
- W3001763584 cites W2088108906 @default.
- W3001763584 cites W2106619117 @default.
- W3001763584 cites W2117757143 @default.
- W3001763584 cites W2118209929 @default.
- W3001763584 cites W2120592477 @default.
- W3001763584 cites W2123032166 @default.
- W3001763584 cites W2125751818 @default.
- W3001763584 cites W2129396106 @default.
- W3001763584 cites W2158265302 @default.
- W3001763584 cites W2164643779 @default.
- W3001763584 cites W2165474932 @default.
- W3001763584 cites W2397048191 @default.
- W3001763584 cites W2411304278 @default.
- W3001763584 cites W2480570015 @default.
- W3001763584 cites W2610429609 @default.
- W3001763584 cites W2897519319 @default.
- W3001763584 cites W2951225413 @default.
- W3001763584 doi "https://doi.org/10.1101/2020.01.21.908897" @default.
- W3001763584 hasPublicationYear "2020" @default.
- W3001763584 type Work @default.
- W3001763584 sameAs 3001763584 @default.
- W3001763584 citedByCount "1" @default.
- W3001763584 countsByYear W30017635842020 @default.
- W3001763584 crossrefType "posted-content" @default.
- W3001763584 hasAuthorship W3001763584A5010167248 @default.
- W3001763584 hasAuthorship W3001763584A5011842501 @default.
- W3001763584 hasAuthorship W3001763584A5014182142 @default.
- W3001763584 hasAuthorship W3001763584A5028071372 @default.
- W3001763584 hasAuthorship W3001763584A5036060606 @default.
- W3001763584 hasAuthorship W3001763584A5056977473 @default.
- W3001763584 hasAuthorship W3001763584A5074140059 @default.
- W3001763584 hasAuthorship W3001763584A5080552339 @default.
- W3001763584 hasAuthorship W3001763584A5083858817 @default.
- W3001763584 hasBestOaLocation W30017635841 @default.
- W3001763584 hasConcept C104317684 @default.
- W3001763584 hasConcept C120821319 @default.
- W3001763584 hasConcept C141231307 @default.
- W3001763584 hasConcept C4918238 @default.
- W3001763584 hasConcept C51679486 @default.
- W3001763584 hasConcept C54355233 @default.
- W3001763584 hasConcept C70721500 @default.
- W3001763584 hasConcept C86803240 @default.
- W3001763584 hasConceptScore W3001763584C104317684 @default.
- W3001763584 hasConceptScore W3001763584C120821319 @default.
- W3001763584 hasConceptScore W3001763584C141231307 @default.
- W3001763584 hasConceptScore W3001763584C4918238 @default.
- W3001763584 hasConceptScore W3001763584C51679486 @default.
- W3001763584 hasConceptScore W3001763584C54355233 @default.
- W3001763584 hasConceptScore W3001763584C70721500 @default.
- W3001763584 hasConceptScore W3001763584C86803240 @default.
- W3001763584 hasLocation W30017635841 @default.
- W3001763584 hasOpenAccess W3001763584 @default.
- W3001763584 hasPrimaryLocation W30017635841 @default.
- W3001763584 hasRelatedWork W14235980 @default.
- W3001763584 hasRelatedWork W15886200 @default.
- W3001763584 hasRelatedWork W23471025 @default.
- W3001763584 hasRelatedWork W26682024 @default.
- W3001763584 hasRelatedWork W26990532 @default.
- W3001763584 hasRelatedWork W29028463 @default.
- W3001763584 hasRelatedWork W33533166 @default.
- W3001763584 hasRelatedWork W37016147 @default.
- W3001763584 hasRelatedWork W4261036 @default.
- W3001763584 hasRelatedWork W21487175 @default.
- W3001763584 isParatext "false" @default.
- W3001763584 isRetracted "false" @default.
- W3001763584 magId "3001763584" @default.
- W3001763584 workType "article" @default.