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- W3002347697 abstract "Engineering and bioconjugation of proteins is a critically valuable tool that can facilitate a wide range of biophysical and structural studies. The ability to orthogonally tag or label a domain within a multidomain protein may be complicated by undesirable side reactions to noninvolved domains. Furthermore, the advantages of segmental (or domain-specific) isotopic labeling for NMR, or deuteration for neutron scattering or diffraction, can be realized by an efficient ligation procedure. Common methods—expressed protein ligation, protein trans-splicing, and native chemical ligation—each have specific limitations. Here, we evaluated the use of different variants of Staphylococcus aureus sortase A for a range of ligation reactions and demonstrate that conditions can readily be optimized to yield high efficiency (i.e. completeness of ligation), ease of purification, and functionality in detergents. These properties may enable joining of single domains into multidomain proteins, lipidation to mimic posttranslational modifications, and formation of cyclic proteins to aid in the development of nanodisc membrane mimetics. We anticipate that the method for ligating separate domains into a single functional multidomain protein reported here may enable many applications in structural biology." @default.
- W3002347697 created "2020-01-30" @default.
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- W3002347697 date "2020-02-01" @default.
- W3002347697 modified "2023-10-18" @default.
- W3002347697 title "Optimization of sortase A ligation for flexible engineering of complex protein systems" @default.
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- W3002347697 doi "https://doi.org/10.1074/jbc.ra119.012039" @default.
- W3002347697 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/7049969" @default.
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