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- W3002571191 abstract "The production of poly-γ-glutamic acid (γ-PGA), a biopolymer consisting of D-and L-glutamic acid monomers, currently relies on L-glutamate or citrate as carbon substrates. Here we aimed at using plant biomass-derived substrates like xylose. γ-PGA producing microorganisms including Bacillus subtilis natively metabolize xylose via the isomerase pathway. The Weimberg pathway, a xylose utilization pathway first described for Caulobacter crescentus, offers a carbon-efficient alternative converting xylose to 2-oxoglutarate without carbon loss. We engineered a recombinant B. subtilis strain that was able to grow on xylose with a growth rate of 0.43 h -1 using a recombinant Weimberg pathway. Although ion-pair reversed-phase LC/MS/MS metabolome analysis revealed lower concentrations of γ-PGA precursors such as 2-oxoglutarate, the γ-PGA titer was increased 6-fold compared to the isomerase strain. Further metabolome analysis indicates a metabolic bottleneck in the phosphoenolpyruvate-pyruvate-oxaloacetate node causing bi-phasic (diauxic) growth of the recombinant Weimberg strain. Flux balance analysis of the γ-PGA producing B. subtilis indicated that a maximal theoretical γ-PGA yield is achieved on D-xylose/D-glucose mixtures. The results of the B. subtilis strain harbouring the Weimberg pathway on such D-xylose/D-glucose mixtures demonstrate indeed resource efficient, high yield γ-PGA production from biomass-derived substrates." @default.
- W3002571191 created "2020-01-30" @default.
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- W3002571191 date "2020-01-21" @default.
- W3002571191 modified "2023-10-14" @default.
- W3002571191 title "Comparison of Isomerase and Weimberg Pathway for γ-PGA Production From Xylose by Engineered Bacillus subtilis" @default.
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- W3002571191 doi "https://doi.org/10.3389/fbioe.2019.00476" @default.
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