FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W3003115100> ?p ?o ?g. }

• W3003115100 abstract "ABSTRACT Currently approved therapeutic monoclonal antibodies (mAbs) are based on immunoglobulin G (IgG) 1, 2 or 4 isotypes, which differ in their specific inter-chains disulfide bridge connectivities. Different analytical techniques have been reported for mAb isotyping, among which native ion mobility mass spectrometry (IM-MS) and collision induced unfolding (CIU) experiments. However, mAb isotyping by these approaches is based on detection of subtle differences and thus remains challenging at the intactlevel. We report here on middle-level (after IdeS digestion) IM-MS and CIU approaches to afford better differentiation of mAb isotypes. Our method provides simultaneously CIU patterns of F(ab’) 2 and Fc domains within a single run. Middle-level CIU patterns of F(ab’)2 domains enable more reliable classification of mAb isotypes compared to intact level CIU, while CIU fingerprints of Fc domains are overall less informative for mAb isotyping. F(ab’) 2 regions can thus be considered as diagnostic domains providing specific CIU signatures for mAb isotyping. Benefits of middle-level IM-MS and CIU approaches are further illustrated on the hybrid IgG2/IgG4 eculizumab. While classical analytical techniques led to controversial results, middle-level CIU uniquely allowed to face the challenge of eculizumab « hybridicity », highlighting that its F(ab’) 2 and Fc CIU patterns corresponds to an IgG2 and an IgG4, respectively. Altogether, the middle-level CIU approach is more clear-cut, accurate and straightforward for canonical but also more complex, engineered next generation mAb formats isotyping. Middle-level CIU thus constitutes a real breakthrough for therapeutic protein analysis, paving the way for its implementation in R&D laboratories." @default.
• W3003115100 created "2020-01-30" @default.
• W3003115100 creator A5003537563 @default.
• W3003115100 creator A5012634736 @default.
• W3003115100 creator A5022292935 @default.
• W3003115100 creator A5033022565 @default.
• W3003115100 creator A5033425192 @default.
• W3003115100 creator A5057339625 @default.
• W3003115100 creator A5066877921 @default.
• W3003115100 creator A5071040855 @default.
• W3003115100 creator A5080276295 @default.
• W3003115100 date "2020-01-21" @default.
• W3003115100 modified "2023-09-24" @default.
• W3003115100 title "Middle-level IM-MS and CIU experiments for improved therapeutic immunoglobulin isotype fingerprinting" @default.
• W3003115100 cites W1555361166 @default.
• W3003115100 cites W1965066688 @default.
• W3003115100 cites W1965638995 @default.
• W3003115100 cites W1970471856 @default.
• W3003115100 cites W1974915370 @default.
• W3003115100 cites W1976636554 @default.
• W3003115100 cites W1993963082 @default.
• W3003115100 cites W2004290717 @default.
• W3003115100 cites W2005919833 @default.
• W3003115100 cites W2023842988 @default.
• W3003115100 cites W2039125944 @default.
• W3003115100 cites W2045712777 @default.
• W3003115100 cites W2057936669 @default.
• W3003115100 cites W2065843920 @default.
• W3003115100 cites W2075219903 @default.
• W3003115100 cites W2079064937 @default.
• W3003115100 cites W2091229935 @default.
• W3003115100 cites W2096387523 @default.
• W3003115100 cites W2148180423 @default.
• W3003115100 cites W2153600773 @default.
• W3003115100 cites W2164143412 @default.
• W3003115100 cites W2257999935 @default.
• W3003115100 cites W2332649031 @default.
• W3003115100 cites W2408391104 @default.
• W3003115100 cites W2597045666 @default.
• W3003115100 cites W2605598976 @default.
• W3003115100 cites W2810391702 @default.
• W3003115100 cites W2894987676 @default.
• W3003115100 cites W2903157281 @default.
• W3003115100 cites W2913213802 @default.
• W3003115100 cites W2913431089 @default.
• W3003115100 cites W2924172316 @default.
• W3003115100 cites W2948242199 @default.
• W3003115100 cites W2950151451 @default.
• W3003115100 doi "https://doi.org/10.1101/2020.01.20.911750" @default.
• W3003115100 hasPublicationYear "2020" @default.
• W3003115100 type Work @default.
• W3003115100 sameAs 3003115100 @default.
• W3003115100 citedByCount "0" @default.
• W3003115100 crossrefType "posted-content" @default.
• W3003115100 hasAuthorship W3003115100A5003537563 @default.
• W3003115100 hasAuthorship W3003115100A5012634736 @default.
• W3003115100 hasAuthorship W3003115100A5022292935 @default.
• W3003115100 hasAuthorship W3003115100A5033022565 @default.
• W3003115100 hasAuthorship W3003115100A5033425192 @default.
• W3003115100 hasAuthorship W3003115100A5057339625 @default.
• W3003115100 hasAuthorship W3003115100A5066877921 @default.
• W3003115100 hasAuthorship W3003115100A5071040855 @default.
• W3003115100 hasAuthorship W3003115100A5080276295 @default.
• W3003115100 hasBestOaLocation W30031151001 @default.
• W3003115100 hasConcept C159654299 @default.
• W3003115100 hasConcept C185592680 @default.
• W3003115100 hasConcept C203014093 @default.
• W3003115100 hasConcept C27256138 @default.
• W3003115100 hasConcept C2777991845 @default.
• W3003115100 hasConcept C542903549 @default.
• W3003115100 hasConcept C55493867 @default.
• W3003115100 hasConcept C70721500 @default.
• W3003115100 hasConcept C71924100 @default.
• W3003115100 hasConcept C86803240 @default.
• W3003115100 hasConceptScore W3003115100C159654299 @default.
• W3003115100 hasConceptScore W3003115100C185592680 @default.
• W3003115100 hasConceptScore W3003115100C203014093 @default.
• W3003115100 hasConceptScore W3003115100C27256138 @default.
• W3003115100 hasConceptScore W3003115100C2777991845 @default.
• W3003115100 hasConceptScore W3003115100C542903549 @default.
• W3003115100 hasConceptScore W3003115100C55493867 @default.
• W3003115100 hasConceptScore W3003115100C70721500 @default.
• W3003115100 hasConceptScore W3003115100C71924100 @default.
• W3003115100 hasConceptScore W3003115100C86803240 @default.
• W3003115100 hasLocation W30031151001 @default.
• W3003115100 hasOpenAccess W3003115100 @default.
• W3003115100 hasPrimaryLocation W30031151001 @default.
• W3003115100 hasRelatedWork W10418545 @default.
• W3003115100 hasRelatedWork W14227563 @default.
• W3003115100 hasRelatedWork W17976133 @default.
• W3003115100 hasRelatedWork W18211028 @default.
• W3003115100 hasRelatedWork W20720251 @default.
• W3003115100 hasRelatedWork W27102347 @default.
• W3003115100 hasRelatedWork W28824784 @default.
• W3003115100 hasRelatedWork W29888127 @default.
• W3003115100 hasRelatedWork W31075329 @default.
• W3003115100 hasRelatedWork W18283075 @default.
• W3003115100 isParatext "false" @default.
• W3003115100 isRetracted "false" @default.
• W3003115100 magId "3003115100" @default.

• next