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- W3003120241 endingPage "101377" @default.
- W3003120241 startingPage "101377" @default.
- W3003120241 abstract "The relapsing fever spirochete Borrelia hermsii and the Lyme disease spirochete Borrelia burgdorferi sensu stricto each produces an abundant, orthologous, outer membrane protein, Vtp and OspC, respectively, when transmitted by tick bite. Gene inactivation studies have shown that both proteins are essential for spirochete infectivity when transmitted by their respective tick vectors. Therefore, we transformed a vtp-minus mutant of B. hermsii with ospC from B. burgdorferi and examined the behavior of this transgenic spirochete in its soft tick vector Ornithodoros hermsi. IFA staining indicated up to 97.8 % of the transgenic B. hermsii upregulated OspC in the ticks’ salivary glands compared to no more than 12.8 % in the midgut, similar to our previous findings with wild-type B. hermsii producing Vtp. Transformation with ospC also restored B. hermsii infectivity to mice when fed upon by infected ticks. Previous sequence analysis of Vtp for 79 isolates and DNA samples of B. hermsii in our laboratory showed this protein is highly polymorphic with 9 divergent amino acid types, yet strikingly the signal peptide is identical among all samples and the same for all OspC signal peptides for B. burgdorferi and related species examined to date. Searches in multiple genome sequences for other species of relapsing fever spirochetes failed to find the same signal peptide sequence to help identify potential transmission-associated proteins. However, some candidate signal peptides with highly similar sequences were found and worthy of future efforts with other species. While OspC of B. burgdorferi restored infectivity to a Vtp-minus mutant of B. hermsii, the functions of these proteins are not known. Our results should stimulate investigators to search for orthologous transmission-associated proteins in other tick-borne spirochetes to better understand how this group of pathogens has coevolved with diverse tick vectors." @default.
- W3003120241 created "2020-01-30" @default.
- W3003120241 creator A5008943614 @default.
- W3003120241 creator A5017915237 @default.
- W3003120241 creator A5090256363 @default.
- W3003120241 date "2020-05-01" @default.
- W3003120241 modified "2023-10-03" @default.
- W3003120241 title "Transgenic functional complementation with a transmission -associated protein restores spirochete infectivity by tick bite" @default.
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- W3003120241 doi "https://doi.org/10.1016/j.ttbdis.2020.101377" @default.
- W3003120241 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/32005628" @default.
- W3003120241 hasPublicationYear "2020" @default.
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