FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W3003334393> ?p ?o ?g. }

• W3003334393 endingPage "2373" @default.
• W3003334393 startingPage "2358" @default.
• W3003334393 abstract "Invadopodia formation is a key driver of cancer metastasis. The noncanonical IkB-related kinase IKKe has been implicated in cancer metastasis, but its roles in invadopodia formation and colorectal cancer (CRC) metastasis are unclear. Methods: Immunofluorescence, gelatin-degradation assay, wound healing assay and transwell invasion assay were used to determine the influence of IKKe over-expression, knockdown and pharmacological inhibition on invadopodia formation and the migratory and invasive capacity of CRC cells in vitro. Effects of IKKe knockdown or pharmacological inhibition on CRC metastasis were examined in mice. Immunohistochemistry staining was used to detect expression levels of IKKe in CRC patient tissues, and its association with prognosis in CRC patients was also analyzed. Immunoprecipitation, western blotting and in vitro kinase assay were constructed to investigate the molecular mechanisms. Results: IKKe co-localizes with F-actin and the invadopodia marker Tks5 at the gelatin-degrading sites of CRC cells. Genetic over-expression/knockdown or pharmacological inhibition of IKKe altered invadopodia formation and the migratory and invasive capacity of CRC cells in vitro. In vivo, knockdown or pharmacological inhibition of IKKe significantly suppressed metastasis of CRC cells in mice. IKKe knockdown also inhibited invadopodia formation in vivo. Clinical investigation of tumor specimens from 191 patients with CRC revealed that high IKKe expression correlates with metastasis and poor prognosis of CRC. Mechanistically, IKKe directly binds to and phosphorylates kindlin-2 at serine 159; this effect mediates the IKKe-induced invadopodia formation and promotion of CRC metastasis. Conclusions: We identify IKKe as a novel regulator of invadopodia formation and a unique mechanism by which IKKe promotes the metastasis of CRC. Our study suggests that IKKe is a potential target to suppress CRC metastasis." @default.
• W3003334393 created "2020-02-07" @default.
• W3003334393 creator A5003261759 @default.
• W3003334393 creator A5007723177 @default.
• W3003334393 creator A5025601401 @default.
• W3003334393 creator A5028780272 @default.
• W3003334393 creator A5030956300 @default.
• W3003334393 creator A5031801305 @default.
• W3003334393 creator A5042618679 @default.
• W3003334393 creator A5045208994 @default.
• W3003334393 creator A5061570552 @default.
• W3003334393 creator A5064376545 @default.
• W3003334393 creator A5072175419 @default.
• W3003334393 creator A5081121957 @default.
• W3003334393 date "2020-01-01" @default.
• W3003334393 modified "2023-10-15" @default.
• W3003334393 title "IKKε phosphorylates kindlin-2 to induce invadopodia formation and promote colorectal cancer metastasis" @default.
• W3003334393 cites W1835883029 @default.
• W3003334393 cites W1977709885 @default.
• W3003334393 cites W1982839259 @default.
• W3003334393 cites W1984291872 @default.
• W3003334393 cites W1988787904 @default.
• W3003334393 cites W1997774562 @default.
• W3003334393 cites W2016899606 @default.
• W3003334393 cites W2023506783 @default.
• W3003334393 cites W2040021772 @default.
• W3003334393 cites W2041644139 @default.
• W3003334393 cites W2051940224 @default.
• W3003334393 cites W2064996695 @default.
• W3003334393 cites W2069261679 @default.
• W3003334393 cites W2070774814 @default.
• W3003334393 cites W2076477722 @default.
• W3003334393 cites W2079332279 @default.
• W3003334393 cites W2092180337 @default.
• W3003334393 cites W2093116529 @default.
• W3003334393 cites W2094295288 @default.
• W3003334393 cites W2102693354 @default.
• W3003334393 cites W2103063473 @default.
• W3003334393 cites W2104105936 @default.
• W3003334393 cites W2105701482 @default.
• W3003334393 cites W2111093256 @default.
• W3003334393 cites W2112229259 @default.
• W3003334393 cites W2112992618 @default.
• W3003334393 cites W2117116688 @default.
• W3003334393 cites W2117609391 @default.
• W3003334393 cites W2121325716 @default.
• W3003334393 cites W2122647599 @default.
• W3003334393 cites W2131052084 @default.
• W3003334393 cites W2131309206 @default.
• W3003334393 cites W2154561072 @default.
• W3003334393 cites W2157073440 @default.
• W3003334393 cites W2163632517 @default.
• W3003334393 cites W2277501221 @default.
• W3003334393 cites W2464870465 @default.
• W3003334393 cites W2482693134 @default.
• W3003334393 cites W2596967923 @default.
• W3003334393 cites W2605760313 @default.
• W3003334393 cites W2605986786 @default.
• W3003334393 cites W2608722406 @default.
• W3003334393 cites W2613997270 @default.
• W3003334393 cites W2738571822 @default.
• W3003334393 cites W2753960063 @default.
• W3003334393 cites W2757119252 @default.
• W3003334393 cites W2771009147 @default.
• W3003334393 cites W2786221326 @default.
• W3003334393 cites W2794441509 @default.
• W3003334393 cites W2806142898 @default.
• W3003334393 cites W2889139530 @default.
• W3003334393 cites W2889646458 @default.
• W3003334393 cites W2890787608 @default.
• W3003334393 cites W2905258543 @default.
• W3003334393 cites W2916816219 @default.
• W3003334393 cites W2925274850 @default.
• W3003334393 cites W2997569490 @default.
• W3003334393 doi "https://doi.org/10.7150/thno.40397" @default.
• W3003334393 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/7019159" @default.
• W3003334393 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/32104508" @default.
• W3003334393 hasPublicationYear "2020" @default.
• W3003334393 type Work @default.
• W3003334393 sameAs 3003334393 @default.
• W3003334393 citedByCount "12" @default.
• W3003334393 countsByYear W30033343932020 @default.
• W3003334393 countsByYear W30033343932021 @default.
• W3003334393 countsByYear W30033343932022 @default.
• W3003334393 countsByYear W30033343932023 @default.
• W3003334393 crossrefType "journal-article" @default.
• W3003334393 hasAuthorship W3003334393A5003261759 @default.
• W3003334393 hasAuthorship W3003334393A5007723177 @default.
• W3003334393 hasAuthorship W3003334393A5025601401 @default.
• W3003334393 hasAuthorship W3003334393A5028780272 @default.
• W3003334393 hasAuthorship W3003334393A5030956300 @default.
• W3003334393 hasAuthorship W3003334393A5031801305 @default.
• W3003334393 hasAuthorship W3003334393A5042618679 @default.
• W3003334393 hasAuthorship W3003334393A5045208994 @default.
• W3003334393 hasAuthorship W3003334393A5061570552 @default.
• W3003334393 hasAuthorship W3003334393A5064376545 @default.
• W3003334393 hasAuthorship W3003334393A5072175419 @default.
• W3003334393 hasAuthorship W3003334393A5081121957 @default.

• next