Matches in SemOpenAlex for { <https://semopenalex.org/work/W3003421078> ?p ?o ?g. }
Showing items 1 to 92 of
92
with 100 items per page.
- W3003421078 endingPage "51" @default.
- W3003421078 startingPage "35" @default.
- W3003421078 abstract "With the advent of widely accessible and cost-effective next-generation sequencing technologies, it has become increasingly feasible to study insect immunity on a deep genomic or transcriptomic level. Here we introduce a protocol that is aimed at exploiting transcriptomic data to study immunity in non-model insect organisms. We provide instructions for an entire workflow, starting with successful extraction of insect RNA through to bioinformatic guidelines for the effective analysis of mRNA sequencing data. The RNA extraction procedure is based on TRIzol Reagent and a spin-column clean-up step. The bioinformatic pipeline is intended to help users identify immune genes from de novo transcriptome data and includes guidelines for conducting differential gene expression analyses on transcriptomic data. The immune gene prediction method is based on inferring protein homologs with HMMER and Blastp and takes advantage of the ImmunoDB database, which is a valuable resource for research on insect immune-related genes and gene families. The differential gene expression analysis procedure utilizes the DESeq2 package as implemented in R. We hope this protocol will serve as a useful resource for researchers aiming to study immunity in non-model insect species." @default.
- W3003421078 created "2020-02-07" @default.
- W3003421078 creator A5030956807 @default.
- W3003421078 creator A5037858737 @default.
- W3003421078 creator A5077664992 @default.
- W3003421078 date "2020-01-01" @default.
- W3003421078 modified "2023-10-13" @default.
- W3003421078 title "Analyzing Immunity in Non-model Insects Using De Novo Transcriptomics" @default.
- W3003421078 cites W1982264720 @default.
- W3003421078 cites W1991894986 @default.
- W3003421078 cites W1999574084 @default.
- W3003421078 cites W2011657487 @default.
- W3003421078 cites W2040360437 @default.
- W3003421078 cites W2055043387 @default.
- W3003421078 cites W2055807373 @default.
- W3003421078 cites W2079955869 @default.
- W3003421078 cites W2098357503 @default.
- W3003421078 cites W2102122585 @default.
- W3003421078 cites W2102549002 @default.
- W3003421078 cites W2102652793 @default.
- W3003421078 cites W2114104545 @default.
- W3003421078 cites W2126419817 @default.
- W3003421078 cites W2134526812 @default.
- W3003421078 cites W2137526110 @default.
- W3003421078 cites W2138207763 @default.
- W3003421078 cites W2151209095 @default.
- W3003421078 cites W2151831732 @default.
- W3003421078 cites W2154775341 @default.
- W3003421078 cites W2158714788 @default.
- W3003421078 cites W2179438025 @default.
- W3003421078 cites W2197124664 @default.
- W3003421078 cites W2198497392 @default.
- W3003421078 cites W2306522809 @default.
- W3003421078 cites W2323326409 @default.
- W3003421078 cites W2592811885 @default.
- W3003421078 cites W2759820149 @default.
- W3003421078 cites W2898402099 @default.
- W3003421078 cites W2938574745 @default.
- W3003421078 cites W3136918052 @default.
- W3003421078 doi "https://doi.org/10.1007/978-1-0716-0259-1_2" @default.
- W3003421078 hasPublicationYear "2020" @default.
- W3003421078 type Work @default.
- W3003421078 sameAs 3003421078 @default.
- W3003421078 citedByCount "1" @default.
- W3003421078 countsByYear W30034210782023 @default.
- W3003421078 crossrefType "book-chapter" @default.
- W3003421078 hasAuthorship W3003421078A5030956807 @default.
- W3003421078 hasAuthorship W3003421078A5037858737 @default.
- W3003421078 hasAuthorship W3003421078A5077664992 @default.
- W3003421078 hasConcept C104317684 @default.
- W3003421078 hasConcept C112190416 @default.
- W3003421078 hasConcept C150194340 @default.
- W3003421078 hasConcept C162317418 @default.
- W3003421078 hasConcept C177212765 @default.
- W3003421078 hasConcept C205713591 @default.
- W3003421078 hasConcept C41008148 @default.
- W3003421078 hasConcept C54355233 @default.
- W3003421078 hasConcept C67705224 @default.
- W3003421078 hasConcept C70721500 @default.
- W3003421078 hasConcept C77088390 @default.
- W3003421078 hasConcept C86803240 @default.
- W3003421078 hasConceptScore W3003421078C104317684 @default.
- W3003421078 hasConceptScore W3003421078C112190416 @default.
- W3003421078 hasConceptScore W3003421078C150194340 @default.
- W3003421078 hasConceptScore W3003421078C162317418 @default.
- W3003421078 hasConceptScore W3003421078C177212765 @default.
- W3003421078 hasConceptScore W3003421078C205713591 @default.
- W3003421078 hasConceptScore W3003421078C41008148 @default.
- W3003421078 hasConceptScore W3003421078C54355233 @default.
- W3003421078 hasConceptScore W3003421078C67705224 @default.
- W3003421078 hasConceptScore W3003421078C70721500 @default.
- W3003421078 hasConceptScore W3003421078C77088390 @default.
- W3003421078 hasConceptScore W3003421078C86803240 @default.
- W3003421078 hasLocation W30034210781 @default.
- W3003421078 hasOpenAccess W3003421078 @default.
- W3003421078 hasPrimaryLocation W30034210781 @default.
- W3003421078 hasRelatedWork W2024780559 @default.
- W3003421078 hasRelatedWork W2091171405 @default.
- W3003421078 hasRelatedWork W2137548031 @default.
- W3003421078 hasRelatedWork W2371817277 @default.
- W3003421078 hasRelatedWork W2386320348 @default.
- W3003421078 hasRelatedWork W2390880853 @default.
- W3003421078 hasRelatedWork W2806739216 @default.
- W3003421078 hasRelatedWork W3102962722 @default.
- W3003421078 hasRelatedWork W3193416841 @default.
- W3003421078 hasRelatedWork W575225911 @default.
- W3003421078 isParatext "false" @default.
- W3003421078 isRetracted "false" @default.
- W3003421078 magId "3003421078" @default.
- W3003421078 workType "book-chapter" @default.