Matches in SemOpenAlex for { <https://semopenalex.org/work/W3003522620> ?p ?o ?g. }
- W3003522620 abstract "Abstract Rewiring cellular sensors to trigger non-natural responses is fundamental for therapeutic cell engineering. Current designs rely on engineered receptors that are limited to single inputs, and often suffer from high leakiness and low fold induction. Here, we present Generalized Engineered Activation Regulators (GEARs) that overcome these limitations by being pathway-specific rather than input-specific. GEARs consist of the MS2 bacteriophage coat protein fused to regulatory or transactivation domains, and work by rerouting activation of the NFAT, NFκB, MAPK or SMAD pathways to dCas9-directed gene expression from genomic loci. This system enables membrane depolarization-induced activation of insulin expression in β-mimetic cells and IL-12 expression in activated Jurkat cells, as well as IL-12 production in response to the immunomodulatory cytokines TGFβ and TNFα in HEK293T cells. Engineered cells with the ability to reinterpret the extracellular milieu have potential for applications in immunotherapy and in the treatment of metabolic diseases." @default.
- W3003522620 created "2020-02-07" @default.
- W3003522620 creator A5030149598 @default.
- W3003522620 creator A5075307711 @default.
- W3003522620 creator A5082252506 @default.
- W3003522620 creator A5091780152 @default.
- W3003522620 date "2020-01-30" @default.
- W3003522620 modified "2023-10-18" @default.
- W3003522620 title "Rewiring of endogenous signaling pathways to genomic targets for therapeutic cell reprogramming" @default.
- W3003522620 cites W1252218738 @default.
- W3003522620 cites W1751510803 @default.
- W3003522620 cites W1753684754 @default.
- W3003522620 cites W1833135536 @default.
- W3003522620 cites W1966291152 @default.
- W3003522620 cites W1968342623 @default.
- W3003522620 cites W1968926710 @default.
- W3003522620 cites W1976783221 @default.
- W3003522620 cites W2002132740 @default.
- W3003522620 cites W2017209592 @default.
- W3003522620 cites W2035743945 @default.
- W3003522620 cites W2036176224 @default.
- W3003522620 cites W2048085771 @default.
- W3003522620 cites W2050169742 @default.
- W3003522620 cites W2067135569 @default.
- W3003522620 cites W2068173904 @default.
- W3003522620 cites W2096810851 @default.
- W3003522620 cites W2097175022 @default.
- W3003522620 cites W2099570622 @default.
- W3003522620 cites W2127862779 @default.
- W3003522620 cites W2133897272 @default.
- W3003522620 cites W2135637759 @default.
- W3003522620 cites W2139231656 @default.
- W3003522620 cites W2152283027 @default.
- W3003522620 cites W2152505144 @default.
- W3003522620 cites W2162250288 @default.
- W3003522620 cites W2168091280 @default.
- W3003522620 cites W2221404162 @default.
- W3003522620 cites W2295326929 @default.
- W3003522620 cites W2318215250 @default.
- W3003522620 cites W2328469110 @default.
- W3003522620 cites W2410547162 @default.
- W3003522620 cites W2475167059 @default.
- W3003522620 cites W2559919850 @default.
- W3003522620 cites W2561362082 @default.
- W3003522620 cites W2755883063 @default.
- W3003522620 cites W2790362095 @default.
- W3003522620 cites W2790571094 @default.
- W3003522620 cites W2791064759 @default.
- W3003522620 cites W2792359172 @default.
- W3003522620 cites W2802363588 @default.
- W3003522620 cites W2806001790 @default.
- W3003522620 cites W2890716371 @default.
- W3003522620 cites W2945141339 @default.
- W3003522620 cites W2951926033 @default.
- W3003522620 cites W2952851245 @default.
- W3003522620 cites W4255760236 @default.
- W3003522620 doi "https://doi.org/10.1038/s41467-020-14397-8" @default.
- W3003522620 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/6992713" @default.
- W3003522620 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/32001704" @default.
- W3003522620 hasPublicationYear "2020" @default.
- W3003522620 type Work @default.
- W3003522620 sameAs 3003522620 @default.
- W3003522620 citedByCount "21" @default.
- W3003522620 countsByYear W30035226202020 @default.
- W3003522620 countsByYear W30035226202021 @default.
- W3003522620 countsByYear W30035226202022 @default.
- W3003522620 countsByYear W30035226202023 @default.
- W3003522620 crossrefType "journal-article" @default.
- W3003522620 hasAuthorship W3003522620A5030149598 @default.
- W3003522620 hasAuthorship W3003522620A5075307711 @default.
- W3003522620 hasAuthorship W3003522620A5082252506 @default.
- W3003522620 hasAuthorship W3003522620A5091780152 @default.
- W3003522620 hasBestOaLocation W30035226201 @default.
- W3003522620 hasConcept C104317684 @default.
- W3003522620 hasConcept C1292079 @default.
- W3003522620 hasConcept C1491633281 @default.
- W3003522620 hasConcept C150194340 @default.
- W3003522620 hasConcept C170493617 @default.
- W3003522620 hasConcept C174510640 @default.
- W3003522620 hasConcept C179464577 @default.
- W3003522620 hasConcept C189938988 @default.
- W3003522620 hasConcept C2776090121 @default.
- W3003522620 hasConcept C2781080636 @default.
- W3003522620 hasConcept C54355233 @default.
- W3003522620 hasConcept C57074206 @default.
- W3003522620 hasConcept C62478195 @default.
- W3003522620 hasConcept C77255625 @default.
- W3003522620 hasConcept C86339819 @default.
- W3003522620 hasConcept C86803240 @default.
- W3003522620 hasConcept C8891405 @default.
- W3003522620 hasConcept C95444343 @default.
- W3003522620 hasConceptScore W3003522620C104317684 @default.
- W3003522620 hasConceptScore W3003522620C1292079 @default.
- W3003522620 hasConceptScore W3003522620C1491633281 @default.
- W3003522620 hasConceptScore W3003522620C150194340 @default.
- W3003522620 hasConceptScore W3003522620C170493617 @default.
- W3003522620 hasConceptScore W3003522620C174510640 @default.
- W3003522620 hasConceptScore W3003522620C179464577 @default.
- W3003522620 hasConceptScore W3003522620C189938988 @default.
- W3003522620 hasConceptScore W3003522620C2776090121 @default.