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• W3003708030 endingPage "1585" @default.
• W3003708030 startingPage "1576" @default.
• W3003708030 abstract "Abstract Rapid and accurate purification of various heterogeneous mixtures is a critical step for a multitude of molecular, chemical, and biological applications. Dielectrophoresis has shown to be a promising technique for particle separation due to its exploitation of the intrinsic electrical properties, simple fabrication, and low cost. Here, we present a geometrically novel dielectrophoretic channel design which utilizes an array of localized electric fields to separate a variety of unique particle mixtures into distinct populations. This label‐free device incorporates multiple winding rows with several nonuniform structures on to sidewalls to produce high electric field gradients, enabling high locally generated dielectrophoretic forces. A balance between dielectrophoretic forces and Stokes’ drag is used to effectively isolate each particle population. Mixtures of polystyrene beads (500 nm and 2 μm), breast cancer cells spiked in whole blood, and for the first time, neuron and satellite glial cells were used to study the separation capabilities of the design. We found that our device was able to rapidly separate unique particle populations with over 90% separation yields for each investigated mixture. The unique architecture of the device uses passivated‐electrode insulator‐based dielectrophoresis in an innovative microfluidic device to separate a variety of heterogeneous mixture without particle saturation in the channel." @default.
• W3003708030 created "2020-02-07" @default.
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• W3003708030 date "2020-02-16" @default.
• W3003708030 modified "2023-10-14" @default.
• W3003708030 title "Passivated‐electrode insulator‐based dielectrophoretic separation of heterogeneous cell mixtures" @default.
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• W3003708030 doi "https://doi.org/10.1002/jssc.201900553" @default.
• W3003708030 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/31991043" @default.
• W3003708030 hasPublicationYear "2020" @default.
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