Matches in SemOpenAlex for { <https://semopenalex.org/work/W3003987990> ?p ?o ?g. }
- W3003987990 abstract "ABSTRACT A major proportion of extracellular RNAs (exRNAs) do not co-isolate with extracellular vesicles (EVs) and remain in ultracentrifugation supernatants of cell-conditioned medium or mammalian blood serum. However, little is known about exRNAs beyond EVs. We have previously shown that the composition of the nonvesicular exRNA fraction is highly biased toward specific tRNA-derived fragments capable of forming RNase-protecting dimers. To solve the problem of stability in exRNA analysis, we developed RI-SEC-seq: a method based on sequencing the size exclusion chromatography (SEC) fractions of nonvesicular extracellular samples treated with RNase inhibitors (RI). This method revealed dramatic compositional changes in exRNA population when enzymatic RNA degradation was inhibited. We demonstrated the presence of ribosomes and full-length tRNAs in cell-conditioned medium of a variety of mammalian cell lines. Their fragmentation generates some small RNAs that are highly resistant to degradation. The extracellular biogenesis of some of the most abundant exRNAs demonstrates that extracellular abundance is not a reliable input to estimate RNA secretion rates. Finally, we showed that chromatographic fractions containing extracellular ribosomes can be sensed by dendritic cells. Extracellular ribosomes and/or tRNAs could therefore be decoded as damage-associated molecular patterns." @default.
- W3003987990 created "2020-02-07" @default.
- W3003987990 creator A5004737680 @default.
- W3003987990 creator A5005752515 @default.
- W3003987990 creator A5009078874 @default.
- W3003987990 creator A5021218220 @default.
- W3003987990 creator A5043038107 @default.
- W3003987990 creator A5049620530 @default.
- W3003987990 creator A5055160872 @default.
- W3003987990 creator A5064469555 @default.
- W3003987990 creator A5066078867 @default.
- W3003987990 creator A5086085093 @default.
- W3003987990 date "2020-01-29" @default.
- W3003987990 modified "2023-09-23" @default.
- W3003987990 title "Fragmentation of extracellular ribosomes and tRNAs shapes the extracellular RNAome" @default.
- W3003987990 cites W1522977578 @default.
- W3003987990 cites W1585322660 @default.
- W3003987990 cites W1661544713 @default.
- W3003987990 cites W1823780969 @default.
- W3003987990 cites W1950118853 @default.
- W3003987990 cites W1965265512 @default.
- W3003987990 cites W1967367827 @default.
- W3003987990 cites W1968057826 @default.
- W3003987990 cites W1970457687 @default.
- W3003987990 cites W1979751563 @default.
- W3003987990 cites W1996075023 @default.
- W3003987990 cites W1997709183 @default.
- W3003987990 cites W2000305484 @default.
- W3003987990 cites W2021612256 @default.
- W3003987990 cites W2024206172 @default.
- W3003987990 cites W2030701238 @default.
- W3003987990 cites W2032696674 @default.
- W3003987990 cites W2033562668 @default.
- W3003987990 cites W2047573465 @default.
- W3003987990 cites W2049702012 @default.
- W3003987990 cites W2055313665 @default.
- W3003987990 cites W2062828595 @default.
- W3003987990 cites W2068332602 @default.
- W3003987990 cites W2081796577 @default.
- W3003987990 cites W2084770914 @default.
- W3003987990 cites W2087463572 @default.
- W3003987990 cites W2108138064 @default.
- W3003987990 cites W2109140791 @default.
- W3003987990 cites W2114147818 @default.
- W3003987990 cites W2119764013 @default.
- W3003987990 cites W2125198788 @default.
- W3003987990 cites W2128573887 @default.
- W3003987990 cites W2128799582 @default.
- W3003987990 cites W2134219545 @default.
- W3003987990 cites W2134940821 @default.
- W3003987990 cites W2141707748 @default.
- W3003987990 cites W2149280513 @default.
- W3003987990 cites W2154479094 @default.
- W3003987990 cites W2167604259 @default.
- W3003987990 cites W2169894204 @default.
- W3003987990 cites W2174471079 @default.
- W3003987990 cites W2239118478 @default.
- W3003987990 cites W2243962757 @default.
- W3003987990 cites W2259481044 @default.
- W3003987990 cites W2296538017 @default.
- W3003987990 cites W2327541311 @default.
- W3003987990 cites W2328294134 @default.
- W3003987990 cites W243816499 @default.
- W3003987990 cites W2509057555 @default.
- W3003987990 cites W2517348684 @default.
- W3003987990 cites W2518959324 @default.
- W3003987990 cites W2532576306 @default.
- W3003987990 cites W2536322725 @default.
- W3003987990 cites W2540034041 @default.
- W3003987990 cites W2545803705 @default.
- W3003987990 cites W2551899789 @default.
- W3003987990 cites W2586195606 @default.
- W3003987990 cites W2588757737 @default.
- W3003987990 cites W2624616322 @default.
- W3003987990 cites W2725858897 @default.
- W3003987990 cites W2742715352 @default.
- W3003987990 cites W2766975063 @default.
- W3003987990 cites W2790701366 @default.
- W3003987990 cites W2793056024 @default.
- W3003987990 cites W2795741191 @default.
- W3003987990 cites W2800397674 @default.
- W3003987990 cites W2803645366 @default.
- W3003987990 cites W2805918213 @default.
- W3003987990 cites W2808496870 @default.
- W3003987990 cites W2883018577 @default.
- W3003987990 cites W2887745160 @default.
- W3003987990 cites W2889644405 @default.
- W3003987990 cites W2895388736 @default.
- W3003987990 cites W2897522485 @default.
- W3003987990 cites W2899540665 @default.
- W3003987990 cites W2903913035 @default.
- W3003987990 cites W2908004981 @default.
- W3003987990 cites W2931164250 @default.
- W3003987990 cites W2931788014 @default.
- W3003987990 cites W2933304441 @default.
- W3003987990 cites W2935163132 @default.
- W3003987990 cites W2939643004 @default.
- W3003987990 cites W2952961321 @default.
- W3003987990 cites W2965521594 @default.
- W3003987990 cites W2969856424 @default.