FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W3004202007> ?p ?o ?g. }

• W3004202007 endingPage "e8491" @default.
• W3004202007 startingPage "e8491" @default.
• W3004202007 abstract "Rapidly growing genetics and bioinformatics studies provide us with an opportunity to obtain a global view of the genetic basis of traits, but also give a challenge to the function validation of candidate genes. CRISPR/Cas9 is an emerging and efficient tool for genome editing. To construct expression clones for the CRISPR/Cas9, most current methods depend on traditional cloning using Gateway reaction or specific type IIS restriction enzymes and DNA ligation, based on multiple steps of PCR. We developed a system for introducing sgRNA expression cassette(s) directly into plant binary vectors in one step. In this system, one sgRNA expression cassette(s) is generated by an optimized multiplex PCR, in which an overlapping PCR took place. Whilst, two sgRNA expression cassettes were amplified in a single round of PCR. Subsequently, an LR or Golden gate reaction was set up with unpurified PCR product and befitting destination vector. We are able to construct expression clones within 36 h, which greatly improves efficiency and saves cost. Furthermore, the efficiency of this system was verified by an agrobacterium-mediated genetic transformation in rice. The system reported here provides a much more efficient and simpler procedure to construct expression clones for CRISPR/Cas9-mediated genome editing." @default.
• W3004202007 created "2020-02-07" @default.
• W3004202007 creator A5005465373 @default.
• W3004202007 creator A5019897854 @default.
• W3004202007 creator A5036106203 @default.
• W3004202007 creator A5039835717 @default.
• W3004202007 creator A5043267366 @default.
• W3004202007 creator A5063049875 @default.
• W3004202007 creator A5087210747 @default.
• W3004202007 date "2020-01-29" @default.
• W3004202007 modified "2023-09-27" @default.
• W3004202007 title "A simple and efficient cloning system for CRISPR/Cas9-mediated genome editing in rice" @default.
• W3004202007 cites W1551141965 @default.
• W3004202007 cites W1892406518 @default.
• W3004202007 cites W1938840550 @default.
• W3004202007 cites W1940605149 @default.
• W3004202007 cites W1991710875 @default.
• W3004202007 cites W1993673609 @default.
• W3004202007 cites W2003171404 @default.
• W3004202007 cites W2026592619 @default.
• W3004202007 cites W2032700288 @default.
• W3004202007 cites W2045435533 @default.
• W3004202007 cites W2055705050 @default.
• W3004202007 cites W2073412341 @default.
• W3004202007 cites W2088816616 @default.
• W3004202007 cites W2116565356 @default.
• W3004202007 cites W2130069008 @default.
• W3004202007 cites W2131978108 @default.
• W3004202007 cites W2132624219 @default.
• W3004202007 cites W2135599428 @default.
• W3004202007 cites W2138098955 @default.
• W3004202007 cites W2142150660 @default.
• W3004202007 cites W2151940278 @default.
• W3004202007 cites W2166934949 @default.
• W3004202007 cites W2290914803 @default.
• W3004202007 cites W2299676134 @default.
• W3004202007 cites W2337751047 @default.
• W3004202007 cites W2506924618 @default.
• W3004202007 cites W2519197628 @default.
• W3004202007 cites W2529689461 @default.
• W3004202007 cites W2555142993 @default.
• W3004202007 cites W2612818194 @default.
• W3004202007 cites W2622596320 @default.
• W3004202007 cites W2665695513 @default.
• W3004202007 cites W2673510916 @default.
• W3004202007 cites W2740956148 @default.
• W3004202007 cites W2765451415 @default.
• W3004202007 cites W2770948545 @default.
• W3004202007 cites W2783749221 @default.
• W3004202007 cites W2786017183 @default.
• W3004202007 cites W2806938093 @default.
• W3004202007 cites W2892052070 @default.
• W3004202007 cites W2895254399 @default.
• W3004202007 cites W2903777469 @default.
• W3004202007 doi "https://doi.org/10.7717/peerj.8491" @default.
• W3004202007 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/6995270" @default.
• W3004202007 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/32030327" @default.
• W3004202007 hasPublicationYear "2020" @default.
• W3004202007 type Work @default.
• W3004202007 sameAs 3004202007 @default.
• W3004202007 citedByCount "11" @default.
• W3004202007 countsByYear W30042020072020 @default.
• W3004202007 countsByYear W30042020072021 @default.
• W3004202007 countsByYear W30042020072022 @default.
• W3004202007 countsByYear W30042020072023 @default.
• W3004202007 crossrefType "journal-article" @default.
• W3004202007 hasAuthorship W3004202007A5005465373 @default.
• W3004202007 hasAuthorship W3004202007A5019897854 @default.
• W3004202007 hasAuthorship W3004202007A5036106203 @default.
• W3004202007 hasAuthorship W3004202007A5039835717 @default.
• W3004202007 hasAuthorship W3004202007A5043267366 @default.
• W3004202007 hasAuthorship W3004202007A5063049875 @default.
• W3004202007 hasAuthorship W3004202007A5087210747 @default.
• W3004202007 hasBestOaLocation W30042020071 @default.
• W3004202007 hasConcept C104317684 @default.
• W3004202007 hasConcept C121050878 @default.
• W3004202007 hasConcept C132455925 @default.
• W3004202007 hasConcept C141231307 @default.
• W3004202007 hasConcept C144501496 @default.
• W3004202007 hasConcept C189819185 @default.
• W3004202007 hasConcept C199360897 @default.
• W3004202007 hasConcept C204241405 @default.
• W3004202007 hasConcept C33161422 @default.
• W3004202007 hasConcept C40767141 @default.
• W3004202007 hasConcept C41008148 @default.
• W3004202007 hasConcept C54355233 @default.
• W3004202007 hasConcept C70721500 @default.
• W3004202007 hasConcept C86803240 @default.
• W3004202007 hasConcept C98108389 @default.
• W3004202007 hasConceptScore W3004202007C104317684 @default.
• W3004202007 hasConceptScore W3004202007C121050878 @default.
• W3004202007 hasConceptScore W3004202007C132455925 @default.
• W3004202007 hasConceptScore W3004202007C141231307 @default.
• W3004202007 hasConceptScore W3004202007C144501496 @default.
• W3004202007 hasConceptScore W3004202007C189819185 @default.
• W3004202007 hasConceptScore W3004202007C199360897 @default.
• W3004202007 hasConceptScore W3004202007C204241405 @default.
• W3004202007 hasConceptScore W3004202007C33161422 @default.

• next