Matches in SemOpenAlex for { <https://semopenalex.org/work/W3004256461> ?p ?o ?g. }
- W3004256461 abstract "Abstract We present in-vivo transplantation of mature dopaminergic neurons by means of macroporous, injectable carriers, to enhance cell therapy in Parkinson’s disease. The carriers are synthesized by crosslinking carboxymethylcellulose at subzero temperatures, resulting in cylindrical, highly resilient porous cryogels, which we term Neurothreads. We develop efficient covalent immobilization of the neural adhesion proteins laminin 111, collagen IV and fibronectin, as well as of the extracellular matrix extract Matrigel to the Neurothreads. We observe the highest neural spreading on laminin 111 and Matrigel. We show compatibility with established dopaminergic differentiation of both HS420 human embryonic stem cells and the LUHMES midbrain model cell line. The porous Neurothread carriers withstand compression during minimally invasive stereotactic injection, and ensure viability of mature neurons including extended neurites. Implanted into the striatum in mice, the Neurothreads enable survival of transplanted mature neurons obtained by directed differentiation of the HS420 human embryonic stem cells, as a dense tissue in situ , including dopaminergic cells. With the successful in-vivo transfer of intact, mature and fully open 3D neural networks, we provide a powerful tool to extend established differentiation protocols to higher maturity and to enhance preconfigured neural network transplantation." @default.
- W3004256461 created "2020-02-07" @default.
- W3004256461 creator A5013782053 @default.
- W3004256461 creator A5019711324 @default.
- W3004256461 creator A5051985484 @default.
- W3004256461 creator A5056051973 @default.
- W3004256461 creator A5059607085 @default.
- W3004256461 creator A5061131551 @default.
- W3004256461 creator A5063413577 @default.
- W3004256461 date "2020-01-31" @default.
- W3004256461 modified "2023-10-18" @default.
- W3004256461 title "Neurothreads: Cryogel carrier-based differentiation and delivery of mature neurons in the treatment of Parkinson’s disease" @default.
- W3004256461 cites W1527930021 @default.
- W3004256461 cites W1576127641 @default.
- W3004256461 cites W1577577364 @default.
- W3004256461 cites W1795809970 @default.
- W3004256461 cites W1861860658 @default.
- W3004256461 cites W1971163936 @default.
- W3004256461 cites W1971912541 @default.
- W3004256461 cites W1972636937 @default.
- W3004256461 cites W1974208182 @default.
- W3004256461 cites W1978648338 @default.
- W3004256461 cites W1985180469 @default.
- W3004256461 cites W1989294686 @default.
- W3004256461 cites W2015648732 @default.
- W3004256461 cites W2020720070 @default.
- W3004256461 cites W2021378086 @default.
- W3004256461 cites W2025494835 @default.
- W3004256461 cites W2031740174 @default.
- W3004256461 cites W2049908093 @default.
- W3004256461 cites W2055919556 @default.
- W3004256461 cites W2056343070 @default.
- W3004256461 cites W2057335179 @default.
- W3004256461 cites W2060932360 @default.
- W3004256461 cites W2061397649 @default.
- W3004256461 cites W2063057031 @default.
- W3004256461 cites W2066375551 @default.
- W3004256461 cites W2068326991 @default.
- W3004256461 cites W2088693883 @default.
- W3004256461 cites W2089996020 @default.
- W3004256461 cites W2091890687 @default.
- W3004256461 cites W2095318835 @default.
- W3004256461 cites W2096772494 @default.
- W3004256461 cites W2102678193 @default.
- W3004256461 cites W2104027922 @default.
- W3004256461 cites W2108410850 @default.
- W3004256461 cites W2125217886 @default.
- W3004256461 cites W2127049662 @default.
- W3004256461 cites W2130128490 @default.
- W3004256461 cites W2136541538 @default.
- W3004256461 cites W2142988817 @default.
- W3004256461 cites W2144885509 @default.
- W3004256461 cites W2190408316 @default.
- W3004256461 cites W2236800972 @default.
- W3004256461 cites W2342539172 @default.
- W3004256461 cites W2419218151 @default.
- W3004256461 cites W2435004623 @default.
- W3004256461 cites W2482794063 @default.
- W3004256461 cites W2506955896 @default.
- W3004256461 cites W2530855489 @default.
- W3004256461 cites W2547385061 @default.
- W3004256461 cites W2557047110 @default.
- W3004256461 cites W2576020053 @default.
- W3004256461 cites W2577227262 @default.
- W3004256461 cites W2586691160 @default.
- W3004256461 cites W2589725017 @default.
- W3004256461 cites W2608361783 @default.
- W3004256461 cites W2611663512 @default.
- W3004256461 cites W2751466955 @default.
- W3004256461 cites W2751773317 @default.
- W3004256461 cites W2765466915 @default.
- W3004256461 cites W2794028436 @default.
- W3004256461 cites W2805543033 @default.
- W3004256461 cites W2811255424 @default.
- W3004256461 cites W2883857116 @default.
- W3004256461 cites W2889216614 @default.
- W3004256461 cites W2901574592 @default.
- W3004256461 cites W2910026324 @default.
- W3004256461 cites W2911974747 @default.
- W3004256461 cites W2938073217 @default.
- W3004256461 cites W2951652912 @default.
- W3004256461 cites W2963220671 @default.
- W3004256461 cites W2985997538 @default.
- W3004256461 cites W4211044101 @default.
- W3004256461 cites W4238941996 @default.
- W3004256461 cites W4246651174 @default.
- W3004256461 cites W4246724958 @default.
- W3004256461 cites W4247312457 @default.
- W3004256461 cites W4289604926 @default.
- W3004256461 cites W4376453125 @default.
- W3004256461 cites W4378610297 @default.
- W3004256461 cites W2105293141 @default.
- W3004256461 doi "https://doi.org/10.1101/2020.01.30.927244" @default.
- W3004256461 hasPublicationYear "2020" @default.
- W3004256461 type Work @default.
- W3004256461 sameAs 3004256461 @default.
- W3004256461 citedByCount "1" @default.
- W3004256461 countsByYear W30042564612021 @default.
- W3004256461 crossrefType "posted-content" @default.
- W3004256461 hasAuthorship W3004256461A5013782053 @default.