Matches in SemOpenAlex for { <https://semopenalex.org/work/W3004490498> ?p ?o ?g. }
- W3004490498 endingPage "1902312" @default.
- W3004490498 startingPage "1902312" @default.
- W3004490498 abstract "Life sciences have been revolutionized by genome editing (GE) tools, including zinc finger nucleases, transcription activator-Like effector nucleases, and CRISPR (clustered regulatory interspaced short palindromic repeats)/Cas (CRISPR-associated) systems, which make the targeted modification of genomic DNA of all organisms possible. CRISPR/Cas systems are being widely used because of their accuracy, efficiency, and cost-effectiveness. Various classes of CRISPR/Cas systems have been developed, but their extensive use may be hindered by off-target effects. Efforts are being made to reduce the off-target effects of CRISPR/Cas9 by generating various CRISPR/Cas systems with high fidelity and accuracy. Several approaches have been applied to detect and evaluate the off-target effects. Here, the current GE tools, the off-target effects generated by GE technology, types of off-target effects, mechanisms of off-target effects, major concerns, and outcomes of off-target effects in plants and animals are summarized. The methods to detect off-target effects, tools for single-guide RNA (sgRNA) design, evaluation and prediction of off-target effects, and strategies to increase the on-target efficiency and mitigate the off-target impact on intended genome-editing outcomes are summarized." @default.
- W3004490498 created "2020-02-14" @default.
- W3004490498 creator A5020726212 @default.
- W3004490498 creator A5031775313 @default.
- W3004490498 creator A5034924979 @default.
- W3004490498 creator A5057814872 @default.
- W3004490498 creator A5073194199 @default.
- W3004490498 creator A5084893043 @default.
- W3004490498 creator A5090618638 @default.
- W3004490498 date "2020-03-01" @default.
- W3004490498 modified "2023-10-16" @default.
- W3004490498 title "CRISPR/Cas Systems in Genome Editing: Methodologies and Tools for sgRNA Design, Off‐Target Evaluation, and Strategies to Mitigate Off‐Target Effects" @default.
- W3004490498 cites W1024663572 @default.
- W3004490498 cites W1553420131 @default.
- W3004490498 cites W1809351293 @default.
- W3004490498 cites W1970196890 @default.
- W3004490498 cites W1974343902 @default.
- W3004490498 cites W1976391205 @default.
- W3004490498 cites W1976404496 @default.
- W3004490498 cites W1986028255 @default.
- W3004490498 cites W1990149668 @default.
- W3004490498 cites W1996390333 @default.
- W3004490498 cites W1997281938 @default.
- W3004490498 cites W1999218372 @default.
- W3004490498 cites W2000046950 @default.
- W3004490498 cites W2001845502 @default.
- W3004490498 cites W2003171404 @default.
- W3004490498 cites W2003797386 @default.
- W3004490498 cites W2006373538 @default.
- W3004490498 cites W2024199022 @default.
- W3004490498 cites W2026520402 @default.
- W3004490498 cites W2030994161 @default.
- W3004490498 cites W2036176224 @default.
- W3004490498 cites W2045435533 @default.
- W3004490498 cites W2050480410 @default.
- W3004490498 cites W2051255304 @default.
- W3004490498 cites W2064815984 @default.
- W3004490498 cites W2077659966 @default.
- W3004490498 cites W2085993152 @default.
- W3004490498 cites W2091772801 @default.
- W3004490498 cites W2095950258 @default.
- W3004490498 cites W2104820165 @default.
- W3004490498 cites W2105464583 @default.
- W3004490498 cites W2107959600 @default.
- W3004490498 cites W2108038398 @default.
- W3004490498 cites W2115603949 @default.
- W3004490498 cites W2116534141 @default.
- W3004490498 cites W2123500370 @default.
- W3004490498 cites W2123746851 @default.
- W3004490498 cites W2123872688 @default.
- W3004490498 cites W2124985265 @default.
- W3004490498 cites W2127862779 @default.
- W3004490498 cites W2128758977 @default.
- W3004490498 cites W2132401721 @default.
- W3004490498 cites W2134268326 @default.
- W3004490498 cites W2139314409 @default.
- W3004490498 cites W2142515819 @default.
- W3004490498 cites W2147114967 @default.
- W3004490498 cites W2151514384 @default.
- W3004490498 cites W2157205823 @default.
- W3004490498 cites W2160051211 @default.
- W3004490498 cites W2165869860 @default.
- W3004490498 cites W2167380395 @default.
- W3004490498 cites W2168740468 @default.
- W3004490498 cites W2173737475 @default.
- W3004490498 cites W2188223075 @default.
- W3004490498 cites W2224398381 @default.
- W3004490498 cites W2252568502 @default.
- W3004490498 cites W2273244674 @default.
- W3004490498 cites W2336346625 @default.
- W3004490498 cites W2336828812 @default.
- W3004490498 cites W2338850193 @default.
- W3004490498 cites W2375351600 @default.
- W3004490498 cites W2396982015 @default.
- W3004490498 cites W2402498701 @default.
- W3004490498 cites W2408159711 @default.
- W3004490498 cites W2410526873 @default.
- W3004490498 cites W2412316478 @default.
- W3004490498 cites W2461013690 @default.
- W3004490498 cites W2511233179 @default.
- W3004490498 cites W2511569157 @default.
- W3004490498 cites W2515083702 @default.
- W3004490498 cites W2531490221 @default.
- W3004490498 cites W2548710592 @default.
- W3004490498 cites W2564226239 @default.
- W3004490498 cites W2565555627 @default.
- W3004490498 cites W2579163968 @default.
- W3004490498 cites W2588613595 @default.
- W3004490498 cites W2603054892 @default.
- W3004490498 cites W2604891032 @default.
- W3004490498 cites W2607962439 @default.
- W3004490498 cites W2611134270 @default.
- W3004490498 cites W2611661371 @default.
- W3004490498 cites W2612451420 @default.
- W3004490498 cites W2619806210 @default.
- W3004490498 cites W2621647941 @default.
- W3004490498 cites W2625522207 @default.
- W3004490498 cites W2726053863 @default.