Matches in SemOpenAlex for { <https://semopenalex.org/work/W3004518672> ?p ?o ?g. }
Showing items 1 to 73 of
73
with 100 items per page.
- W3004518672 abstract "Klebsiella pneumoniae serotype K2, belonging to Enterobacteriaceae family, is one of the common gram negative pathogens. Due to the high prevalence of serotype K2, it is essential to provide the precise, effective and exclusive method to detect the bacteria. In the last few years, molecular methods such as PCR, gold nanoparticles biosensors have been developed for rapid, precise, easy, and inexpensive with high specificity and sensitivity detection of human, plant and animal pathogens diagnosis. In this study, in order to detect the Klebsiella pneumoniae serotype K2, K2A, rmpA and orf10 genes were used. Primers were designed and identification of bacteria was performed by PCR for each gene and Multiplex PCR for orf10 and K2A genes. K2A gene sequencing was done and thiolated oligonucleotide were designed based on the sequencing result. Synthesis of gold nanoparticles with approximately 20 nm diameter was carried out and Klebsiella pneumoniae serotype K2 genomic DNA was used for detection using gold nanoparticles and probes. A 532 bp fragment of K2A gene with sensitivity of 1 pg/µL and 309 bp fragment of orf10 gene with sensitivity of 0.07 ng/µL were amplified by PCR and multiplex PCR, however no band was observed for rmpA gene. Change in color of gold nanoparticles in the presence of K2 serotype genomic DNA and oligonucleotide probes was achieved and maximum changes in wavelength occurred at 550 to 650 nm. Therefore the method could be introduced as a rapid, accurate and inexpensive technique with high specificity compared with the biochemical and molecular techniques for detection of Klebsiella pneumoniae serotype K2." @default.
- W3004518672 created "2020-02-14" @default.
- W3004518672 creator A5040509698 @default.
- W3004518672 date "2015-01-01" @default.
- W3004518672 modified "2023-09-26" @default.
- W3004518672 title "Specific detection of Klebsiella pneumoniae serotype K2 bygold nanoparticles probe" @default.
- W3004518672 hasPublicationYear "2015" @default.
- W3004518672 type Work @default.
- W3004518672 sameAs 3004518672 @default.
- W3004518672 citedByCount "0" @default.
- W3004518672 crossrefType "dissertation" @default.
- W3004518672 hasAuthorship W3004518672A5040509698 @default.
- W3004518672 hasConcept C10389963 @default.
- W3004518672 hasConcept C104317684 @default.
- W3004518672 hasConcept C126322002 @default.
- W3004518672 hasConcept C129312508 @default.
- W3004518672 hasConcept C153911025 @default.
- W3004518672 hasConcept C17757408 @default.
- W3004518672 hasConcept C2777058267 @default.
- W3004518672 hasConcept C2781188995 @default.
- W3004518672 hasConcept C40993552 @default.
- W3004518672 hasConcept C49105822 @default.
- W3004518672 hasConcept C51679486 @default.
- W3004518672 hasConcept C54355233 @default.
- W3004518672 hasConcept C547475151 @default.
- W3004518672 hasConcept C71924100 @default.
- W3004518672 hasConcept C86803240 @default.
- W3004518672 hasConcept C89423630 @default.
- W3004518672 hasConcept C90583042 @default.
- W3004518672 hasConceptScore W3004518672C10389963 @default.
- W3004518672 hasConceptScore W3004518672C104317684 @default.
- W3004518672 hasConceptScore W3004518672C126322002 @default.
- W3004518672 hasConceptScore W3004518672C129312508 @default.
- W3004518672 hasConceptScore W3004518672C153911025 @default.
- W3004518672 hasConceptScore W3004518672C17757408 @default.
- W3004518672 hasConceptScore W3004518672C2777058267 @default.
- W3004518672 hasConceptScore W3004518672C2781188995 @default.
- W3004518672 hasConceptScore W3004518672C40993552 @default.
- W3004518672 hasConceptScore W3004518672C49105822 @default.
- W3004518672 hasConceptScore W3004518672C51679486 @default.
- W3004518672 hasConceptScore W3004518672C54355233 @default.
- W3004518672 hasConceptScore W3004518672C547475151 @default.
- W3004518672 hasConceptScore W3004518672C71924100 @default.
- W3004518672 hasConceptScore W3004518672C86803240 @default.
- W3004518672 hasConceptScore W3004518672C89423630 @default.
- W3004518672 hasConceptScore W3004518672C90583042 @default.
- W3004518672 hasLocation W30045186721 @default.
- W3004518672 hasOpenAccess W3004518672 @default.
- W3004518672 hasPrimaryLocation W30045186721 @default.
- W3004518672 hasRelatedWork W1548770088 @default.
- W3004518672 hasRelatedWork W1728964360 @default.
- W3004518672 hasRelatedWork W1984362263 @default.
- W3004518672 hasRelatedWork W2052674053 @default.
- W3004518672 hasRelatedWork W2109366821 @default.
- W3004518672 hasRelatedWork W2164063072 @default.
- W3004518672 hasRelatedWork W226638698 @default.
- W3004518672 hasRelatedWork W2360493912 @default.
- W3004518672 hasRelatedWork W2363252071 @default.
- W3004518672 hasRelatedWork W2373849278 @default.
- W3004518672 hasRelatedWork W2389655457 @default.
- W3004518672 hasRelatedWork W2392557726 @default.
- W3004518672 hasRelatedWork W2401479975 @default.
- W3004518672 hasRelatedWork W2412209777 @default.
- W3004518672 hasRelatedWork W3020500956 @default.
- W3004518672 hasRelatedWork W2836579167 @default.
- W3004518672 hasRelatedWork W2875242876 @default.
- W3004518672 hasRelatedWork W2939453070 @default.
- W3004518672 hasRelatedWork W3019701328 @default.
- W3004518672 hasRelatedWork W3183109502 @default.
- W3004518672 isParatext "false" @default.
- W3004518672 isRetracted "false" @default.
- W3004518672 magId "3004518672" @default.
- W3004518672 workType "dissertation" @default.