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• W3004586191 endingPage "2484" @default.
• W3004586191 startingPage "2477" @default.
• W3004586191 abstract "Lacto-N-neotetraose (LNnT), one of the oligosaccharides in human milk, has many beneficial effects on infant health. In a recent work, we have constructed a recombinant Bacillus subtilis strain for the production of LNnT. Here, we further improved LNnT production with a xylose-induced clustered regularly interspaced short palindromic repeats interference system. In particular, the expressions of pfkA and pyk genes in the Embden-Meyerhof-Parnas pathway module, zwf gene in the pentose phosphate pathway module, and mnaA gene in the teichoic acid synthesis module were downregulated. The LNnT titer was increased from 1.32 to 1.55 g/L. Furthermore, to improve the conversion efficiency of lacto-N-triose II to LNnT, we knocked out tuaD gene in branch pathway and improved the expression of lgtB gene, resulting in the further increase of LNnT titer to 2.01 g/L. Finally, the addition time and amount of inducer xylose were optimized, and LNnT titer reached 2.30 g/L in shake flask and 5.41 g/L in 3 L bioreactor." @default.
• W3004586191 created "2020-02-14" @default.
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• W3004586191 date "2020-02-04" @default.
• W3004586191 modified "2023-10-15" @default.
• W3004586191 title "CRISPRi-Guided Multiplexed Fine-Tuning of Metabolic Flux for Enhanced Lacto-<i>N</i>-neotetraose Production in <i>Bacillus subtilis</i>" @default.
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• W3004586191 doi "https://doi.org/10.1021/acs.jafc.9b07642" @default.
• W3004586191 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/32013418" @default.
• W3004586191 hasPublicationYear "2020" @default.
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