FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W3004586670> ?p ?o ?g. }

• W3004586670 endingPage "109532" @default.
• W3004586670 startingPage "109532" @default.
• W3004586670 abstract "aurJ3M(GenBank:EU697915.1), a 579 bp gene, whose deduced product (192 amino acid) was found to have amino acid sequence homology with some bacterial regulatory proteins. Computer-assisted analysis showed that AurJ3M is PAS-LuxR family regulatory protein, it combines a PAS domain with a helix-turn-helix (HTH) motif of the LuxR type. Gene deletion of aurJ3M from the S.aureofuscus SYAU0709 through gene replacement with the apramycin resistance gene aac (3) IV and the DNA fragment of the replication initiation site sequence OriT resulted in complete loss of aureofuscin production, indicating that AurJ3M is a positive regulator during the aureofuscin biosynthesis. Gene expression analyses in S.aureofuscus SYAU0709 and S.aureofuscus ΔaurJ3M by reverse transcriptase PCR (RT-PCR) indicated there no transcripts for the genes B、C、G、F、S0、S1、D in S.aureofuscus ΔaurJ3M, maintains some transcription of these genes although the levels is low. Transcription was also significantly reduced for gene A, and no difference in the transcription pattern was observed for the genes R、E and H, a similar transcription pattern was also observed for AurJ3M, A and B showed a different transcription pattern, while transcription of gene A in S.aureofuscus ΔaurJ3M when compared to the parental strain, no transcripts could be detected for gene B in the mutant; This result indicates that the deletion of aurJ3M gene doesn't have a polar effect on the transcription of genes located downstream from aurJ3M. In the ΔaurJ3M knockout mutant, except for the transcription of E、H、M and R, the transcription of other genes in the cluster was down-regulated, HPLC shows there no secondary metabolites was produced,aurJ3M has a pathway-specific regulation effect on the biosynthesis of aureofuscin. Aureofuscin had broad application prospects in food preservation, and enriched the resource pool of anti-fungal antibiotics of tetraphenyl macrolide in China." @default.
• W3004586670 created "2020-02-14" @default.
• W3004586670 creator A5001265165 @default.
• W3004586670 creator A5004169167 @default.
• W3004586670 creator A5011719530 @default.
• W3004586670 creator A5081292377 @default.
• W3004586670 creator A5086763630 @default.
• W3004586670 date "2020-06-01" @default.
• W3004586670 modified "2023-09-24" @default.
• W3004586670 title "Regulation of aureofuscin production by the PAS-LuxR family regulator AurJ3M" @default.
• W3004586670 cites W1585367849 @default.
• W3004586670 cites W1877801295 @default.
• W3004586670 cites W1907264269 @default.
• W3004586670 cites W1964182783 @default.
• W3004586670 cites W1982325415 @default.
• W3004586670 cites W1984476760 @default.
• W3004586670 cites W2005175892 @default.
• W3004586670 cites W2018847934 @default.
• W3004586670 cites W2037644008 @default.
• W3004586670 cites W2041888890 @default.
• W3004586670 cites W2059397761 @default.
• W3004586670 cites W2062965668 @default.
• W3004586670 cites W2072254412 @default.
• W3004586670 cites W2088215858 @default.
• W3004586670 cites W2134639528 @default.
• W3004586670 cites W2171555414 @default.
• W3004586670 cites W2339955562 @default.
• W3004586670 cites W2795201752 @default.
• W3004586670 doi "https://doi.org/10.1016/j.enzmictec.2020.109532" @default.
• W3004586670 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/32423669" @default.
• W3004586670 hasPublicationYear "2020" @default.
• W3004586670 type Work @default.
• W3004586670 sameAs 3004586670 @default.
• W3004586670 citedByCount "3" @default.
• W3004586670 countsByYear W30045866702022 @default.
• W3004586670 crossrefType "journal-article" @default.
• W3004586670 hasAuthorship W3004586670A5001265165 @default.
• W3004586670 hasAuthorship W3004586670A5004169167 @default.
• W3004586670 hasAuthorship W3004586670A5011719530 @default.
• W3004586670 hasAuthorship W3004586670A5081292377 @default.
• W3004586670 hasAuthorship W3004586670A5086763630 @default.
• W3004586670 hasConcept C101762097 @default.
• W3004586670 hasConcept C104317684 @default.
• W3004586670 hasConcept C138885662 @default.
• W3004586670 hasConcept C143065580 @default.
• W3004586670 hasConcept C150194340 @default.
• W3004586670 hasConcept C153911025 @default.
• W3004586670 hasConcept C165864922 @default.
• W3004586670 hasConcept C179926584 @default.
• W3004586670 hasConcept C27153228 @default.
• W3004586670 hasConcept C33498276 @default.
• W3004586670 hasConcept C41895202 @default.
• W3004586670 hasConcept C54355233 @default.
• W3004586670 hasConcept C86339819 @default.
• W3004586670 hasConcept C86803240 @default.
• W3004586670 hasConceptScore W3004586670C101762097 @default.
• W3004586670 hasConceptScore W3004586670C104317684 @default.
• W3004586670 hasConceptScore W3004586670C138885662 @default.
• W3004586670 hasConceptScore W3004586670C143065580 @default.
• W3004586670 hasConceptScore W3004586670C150194340 @default.
• W3004586670 hasConceptScore W3004586670C153911025 @default.
• W3004586670 hasConceptScore W3004586670C165864922 @default.
• W3004586670 hasConceptScore W3004586670C179926584 @default.
• W3004586670 hasConceptScore W3004586670C27153228 @default.
• W3004586670 hasConceptScore W3004586670C33498276 @default.
• W3004586670 hasConceptScore W3004586670C41895202 @default.
• W3004586670 hasConceptScore W3004586670C54355233 @default.
• W3004586670 hasConceptScore W3004586670C86339819 @default.
• W3004586670 hasConceptScore W3004586670C86803240 @default.
• W3004586670 hasFunder F4320321001 @default.
• W3004586670 hasLocation W30045866701 @default.
• W3004586670 hasLocation W30045866702 @default.
• W3004586670 hasOpenAccess W3004586670 @default.
• W3004586670 hasPrimaryLocation W30045866701 @default.
• W3004586670 hasRelatedWork W122230838 @default.
• W3004586670 hasRelatedWork W1870486696 @default.
• W3004586670 hasRelatedWork W1930517486 @default.
• W3004586670 hasRelatedWork W2099645254 @default.
• W3004586670 hasRelatedWork W2172226517 @default.
• W3004586670 hasRelatedWork W2404327720 @default.
• W3004586670 hasRelatedWork W2408793279 @default.
• W3004586670 hasRelatedWork W2417280735 @default.
• W3004586670 hasRelatedWork W2461780163 @default.
• W3004586670 hasRelatedWork W4234043289 @default.
• W3004586670 hasVolume "137" @default.
• W3004586670 isParatext "false" @default.
• W3004586670 isRetracted "false" @default.
• W3004586670 magId "3004586670" @default.
• W3004586670 workType "article" @default.